IF-LCM: Laser capture microdissection of immunofluorescently defined cells for mRNA analysis

Kidney International

Volumn 58, Issue 3, 2000, Pages 1346-1353

  Murakami, H ^a   Liotta, L ^a   Star, R A ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Acridine orange; Immunofluorescence; Microdissection; RNA preservation; Thick ascending limb; Video microscopy

Indexed keywords

AQUAPORIN 2; MESSENGER RNA; TAMM HORSFALL GLYCOPROTEIN;

ACUTE KIDNEY FAILURE; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; DISSECTION; FEMALE; GENE EXPRESSION; IMMUNOFLUORESCENCE MICROSCOPY; KIDNEY; KIDNEY TUBULE; LASER; MOUSE; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0033850140     PISSN: 00852538     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1523-1755.2000.00295.x     Document Type: Article

References (23)

1. Laser capture microdissection: Molecular analysis of tissue
2. Laser capture microdis-section
3. Immuno LCM: Laser capture microdissection of immunostained frozen sections for mRNA analysis
4. Laser-capture microdissection: Opening the microscopic frontier to molecular analysis
5. Analysis of anterior pituitary hormone mRNA expression in immunophenotypically characterized single cells after laser capture microdissection
6. Analysis of segment al renal gene expression by laser capture microdissection
7. Gene expression profiles of laser-captured adjacent neuronal subtypes
8. Construction of a representative cDNA library from prostatic intraepithelial neoplasia
9. Acridine orange-RNA fluorescence of maturing neurons in the perinatal rat brain
10. Sequestration of RNA in Alzheimer's disease neurofibrillary tangles and senile plaques
11. Tamm-Horsfall mucoprotein. I. Localization in the kidney
12. rBAT, related to L-cysteine transport, is localized to the microvilli of proximal straight tubules, and its expression is regulated in kidney by development
13. Cloning and expression of apical membrane water channel of rat kidney collecting tubule
14. Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue
15. Subtraction hybridization cloning of RNA amplified from different cell populations microdissected from cryostat tissue sections
16. In vivo gene expression profile analysis of human breast cancer progression
17. The potential use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis: Preliminary findings
18. An approach to proteomic analysis of human tumors
19. Characterization of intracellular prostate-specific antigen from laser capture microdissected benign and malignant prostatic epithelium
20. Sensitive immunoassay of tissue cell proteins procured by laser capture microdissection
21. Expression of cytokine-like genes JE and KC is increased during renal ischemia
22. Decreased abundance of collecting duct aquaporins in post-ischemic renal failure in rats