Molecular analysis of complex tissues is facilitated by laser capture microdissection: Critical role of upstream tissue processing

Cell Biochemistry and Biophysics

Volumn 35, Issue 1, 2001, Pages 103-113

  Rekhter, Mark D ^a   Chen, Jing ^a  

^a PFIZER INC   (United States)

Author keywords

Histotechnology; Laser capture microdissection; Molecular biology

Indexed keywords

DNA; RNA;

ANIMAL; CELL SEPARATION; DISSECTION; GENETIC PROCEDURES; HUMAN; IMMUNOHISTOCHEMISTRY; INSTRUMENTATION; METHODOLOGY; MICROSCOPY; REVIEW;

ANIMALS; CELL SEPARATION; DISSECTION; DNA; GENETIC TECHNIQUES; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY; RNA;

EID: 0035754164     PISSN: 10859195     EISSN: None     Source Type: Journal    
DOI: 10.1385/CBB:35:1:103     Document Type: Review

References (51)

1. Lockhart, D. J. and Winzeler, E. A. (2000) Genomics, gene expression and DNA arrays. Nature 405, 827-836.
2. Pandey, A. and Mann, M. (2000) Proteomics to study genes and genomes. Nature 405, 837-846.
3. Going, J. J. and Lamb, R. F. (1996) Practical histological microdissection for PCR analysis. J. Pathol. 179, 121-124.
4. Isenberg, G., Bielser, W., Meier, R. W., and Remy, E. (1976) Cell surgery by laser micro-dissection: a preparative method. J. Microsc. 107, 19-24.
5. Bonner, R. F., Emmert, B. M., Cole, K., Pohida, T., Chuaqui, R., Goldstein, S., and Liotta, L. A. (1997) Laser capture microdissection: molecular analysis of tissue. Science 278, 1481-1483.
6. Emmert, B. M., Bonner, R. F., Smith, P. D., Chuaqui, R. F., Zhuang, Z., Goldstein, S. R., et al. (1996) Laser capture microdissection [see comments]. Science 274, 998-1001.
7. Emmert, B. M., Strausberg, R. L., Krizman, D. B., Bonaldo, M. F., Bonner, R. F., Bostwick, D. G., et al. (2000) Molecular profiling of clinical tissue specimens: feasibility and applications. Am. J. Pathol. 156, 1109-1115.
8. Simone, N. L., Bonner, R. F., Gillespie, J. W., Emmert, B. M., and Liotta, L. A. (1998) Laser-capture microdissection: opening the microscopic frontier to molecular analysis. Trends Genet. 14, 272-276.
9. Liotta, L. A. and Petricoin, E. F. (2000) Molecular profiling of human cancer. Nature Rev. Genetics 1, 48-56.
10. Fend, F. and Raffeld, M. (2000) Laser capture microdissection in pathology. J. Clin. Pathol. 53, 666-672.
11. Curran, S., McKay, J. A., McLeod, H. L., and Murray, G. I. (2000) Laser capture microscopy. Mol. Pathol. 53, 64-68.
12. Goldstein, S. R., McQueen, P. G., and Bonner, R. F. (1998) Thermal modeling of laser capture microdissection. Appl. Optics 37, 7378-7391.
13. Schutze, K. and Lahr, G. (1998) Identification of expressed genes by laser-mediated manipulation of single cells [see comments]. Nat. Biotechnol. 16, 737-742.
14. Fink, L., Seeger, W., Ermert, L., Hanze, J., Stahl, U., Grimminger, F., et al. (1998) Real-time quantitative RT-PCR after laser-assisted cell picking. Nat. Med. 4, 1329-1333.
15. Gordon, G. J., Coleman, W. B., and Grisham, J. W. (2000) Temporal analysis of hepatocyte differentiation by small hepatocyte-like progenitor cells during liver regeneration in retrorsine-exposed rats. Am. J. Pathol. 157, 771-786.
16. Maitra, A., Wistuba, I. I., Virmani, A. K., Sakaguchi, M., Park, I., Stucky, A., et al. (1999) Enrichment of epithelial cells for molecular studies. Nat. Med. 5, 459-463.
17. Luo, L., Salunga, R. C., Guo, H., Bittner, A., Joy, K. C., Galindo, J. E., et al. (1999) Gene expression profiles of laser-captured adjacent neuronal subtypes [published erratum appears in Nat Med Mar;5(3):355]. Nat. Med. 5, 117-122.
18. Leethanakul, C., Patel, V., Gillespie, J., Pallente, M., Ensley, J. F., Koontongkaew, S., et al. (2000) Distinct pattern of expression of differentiation and growth-related genes in squamous cell carcinomas of the head and neck revealed by the use of laser capture microdissection and cDNA arrays. Oncogene 19, 3220-3224.
19. Glasow, A., Haidan, A., Hilbers, U., Breidert, M., Gillespie, J., Scherbaum, W. A., et al. (1998) Expression of Ob receptor in normal human adrenals: differential regulation of adrenocortical and adrenomedullary function by leptin. J. Clin. Endocrinol. Metab. 83, 4459-4466.
20. Cole, K. A., Krizman, D. B., and Emmert, B. M. (1999) The genetics of cancer; a 3D model. Nat. Genet. 21, 38-41.
21. Fend, F., Emmert, B. M., Chuaqui, R., Cole, K., Lee, J., Liotta, L. A., and Raffeld, M. (1999) Immuno-LCM: laser capture microdissection of immunostained frozen sections for mRNA analysis. Am. J. Pathol. 154, 61-66.
22. Jin, L., Thompson, C. A., Qian, X., Kuecker, S. J., Kulig, E., and Lloyd, R. V. (1999) Analysis of anterior pituitary hormone mRNA expression in immunophenotypically characterized single cells after laser capture microdissection. Lab. Invest. 79, 511-512.
23. Goldsworthy, S. M., Stockton, P. S., Trempus, C. S., Foley, J. F., and Maronpot, R. R. (1999) Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue. Mol. Carcinog. 25, 86-91.
24. Lahr, G. (2000) RT-PCR from archival single cells is a suitable method to analyze specific gene expression. Lab. Invest. 80, 1477-1479.
25. Fend, F., Quintanilla, M. L., Kumar, S., Beaty, M. W., Blum, L., Sorbara, L., et al. (1999) Composite low grade B-cell lymphomas with two immunophenotypically distinct cell populations are true biclonal lymphomas. A molecular analysis using laser capture microdissection. Am. J. Pathol. 154, 1857-1866.
26. Quintanilla, M. L., Fend, F., Moguel, L. R., Spilove, L., Beaty, M. W., Kingma, D. W., et al. (1999) Peripheral T-cell lymphoma with Reed-Sternberg-like cells of B-cell phenotype and genotype associated with Epstein-Barr virus infection. Am. J. Surg. Pathol. 23, 1233-1240.
27. Tam, A. S., Foley, J. F., Devereux, T. R., Maronpot, R. R., and Massey, T. E. (1999) High frequency and heterogeneous distribution of p53 mutations in aflatoxin B1-induced mouse lung tumors. Cancer Res. 59, 3634-3640.
28. Sawyer, E. J., Hanby, A. M., Ellis, P., Lakhani, S. R., Ellis, I. O., Boyle, S., and Tomlinson, I. P. (2000) Molecular analysis of phyllodes tumors reveals distinct changes in the epithelial and stromal components. Am. J. Pathol. 156, 1093-1098.
29. Sirivatanauksorn, Y., Sirivatanauksorn, V., Bhattacharya, S., Davidson, B. R., Dhillon, A. P., Kakkar, A. K., et al. (1999) Evolution of genetic abnormalities in hepatocellular carcinomas demonstrated by DNA fingerprinting. J. Pathol. 189, 344-350.
30. Shen, C. Y., Yu, J. C., Lo, Y L., Kuo, C. H., Yue, C. T., Jou, Y. S., et al. (2000) Genome-wide search for loss of heterozygosity using laser capture microdissected tissue of breast carcinoma: an implication for mutator phenotype and breast cancer pathogenesis. Cancer Res. 60, 3884-3892.
31. Jones, C., Foschini, M. P., Chaggar, R., Lu, Y. J., Wells, D., Shipley, J. M., et al. (2000) Comparative genomic hybridization analysis of myoepithelial carcinoma of the breast. Lab. Invest. 80, 831-836.
32. Rubin, M. A., Gerstein, A., Reid, K., Bostwick, D. G., Cheng, L., Parsons, R., and Papadopoulos, N. (2000) 10q23.3 loss of heterozygosity is higher in lymph node-positive (pT2-3,N+) versus lymph node-negative (pT2-3,N0) prostate cancer. Hum. Pathol. 31, 504-508.
33. DiFrancesco, L. M., Murthy, S. K., Luider, J., and Demetrick, D. J. (2000) Laser capture microdissection-guided fluorescence in situ hybridization and flow cytometric cell cycle analysis of purified nuclei from paraffin sections. Mod. Pathol. 13, 705-711.
34. Darling, T. N., Yee, C., Bauer, J. W., Hintner, H., and Yancey, K. B. (1999) Revertant mosaicism: partial correction of a germ-line mutation in COL17A1 by a frame-restoring mutation. J. Clin. Invest. 103, 1371-1377.
35. Relman, D. A. (1999) The search for unrecognized pathogens. Science 284, 1308-1310.
36. Shibutani, M., Uneyama, C., Miyazaki, K., Toyoda, K., and Hirose, M. (2000) Methacarn fixation: a novel tool for analysis of gene expressions in paraffin-embedded tissue specimens. Lab. Invest. 80, 199-208.
37. Wong, M. H., Saam, J. R., Stappenbeck, T. S., Rexer, C. H., and Gordon, J. I. (2000) Genetic mosaic analysis based on Cre recombinase and navigated laser capture microdissection. Proc. Natl. Acad. Sci. USA 97, 12,601-12,606.
38. Sgroi, D. C., Teng, S., Robinson, G., LeVangie, R., Hudson-JR, J., and Elkahloun, A. G. (1999) In vivo gene expression profile analysis of human breast cancer progression. Cancer Res. 59, 5656-5661.
39. Ohyama, H., Zhang, X., Kohno, Y., Alevizos, M., Posner, D. T., Wong, D. T., and Todd, A. (2000) Laser capture microdissection-generated target sample for high-density oligonucleotide array hybridization. Biotechniques 29, 530-536.
40. Peterson, L. A., Brown, M. R., Carlisle, A. J., Kohn, E. C., Liotta, L. A., Emmert, B. M., and Krizman, D. B. (1998) An improved method for construction of directionally cloned cDNA libraries from microdissected cells. Cancer Res. 58, 5326-5328.
41. Emmert, B. M., Gillespie, J. W., Paweletz, C. P., Ornstein, D. K., Basrur, V., Appella, E., et al. (2000) An approach to proteomic analysis of human tumors. Mol. Carcinog. 27, 158-165.
42. Ornstein, D. K., Englert, C., Gillespie, J. W., Paweletz, C. P., Linehan, W. M., Emmert, B. M., and Petricoin, E. F. (2000) Characterization of intracellular prostate-specific antigen from laser capture microdissected benign and malignant prostatic epithelium. Clin. Cancer Res. 6, 353-356.
43. Ornstein, D. K., Gillespie, J., Paweletz, C. P., Duray, P. H., Herring, J., Vocke, C. D., et al. (2000) Proteomic analysis of laser capture microdissected human prostate cancer cells and in vitro prostate cell lines. Electrophoresis 21, 2235-2242.
44. Banks, R. E., Dunn, M. J., Forbes, M. A., Stanley, A., Pappin, D., Naven, T., et al. (1999) The potential use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis - preliminary findings. Electrophoresis 20, 689-700.
45. Natkunam, Y., Rouse, R. V., Zhu, S., Fisher, C., and van-De-Rijn, M. (2000) Immunoblot analysis of CD34 expression in histologically diverse neoplasms. Am. J. Pathol. 156, 21-27.
46. Simone, N. L., Remaley, A. T., Charboneau, L., Petricoin, E. F., Glickman, J. W., Emmert, B. M., et al. (2000) Sensitive immunoassay of tissue cell proteins procured by laser capture microdissection. Am. J. Pathol. 156, 445-452.
47. Palmer-Toy, D. E., Sarracino, D. A., Sgroi, D. C., LeVangie, R., and Leopold, P. E. (2000) Direct acquisition of matrix-assisted laser desorption/ ionization time-of-flight mass spectra from laser capture microdissected cells. Clin. Chem. 46, 1513-1516. (Schutze & Lahr 1998 ID: 1064).
48. Godfrey, T. E., Kim, S.-H., Chavira, M., Ruff, D. W., Warren, R. S., Gray, J. W., and Jensen, R. H. (2000) Quantitative mRNA expression analysis from formalin-fixed, paraffin-embedded tissues using 5′ nuclease quantitative reverse transcription-polymerase chain reaction. J. Mol. Diag. 2, 84-91.
49. Specht, K., Richter, T., Muller, U., Walch, A., Werner, M., and Hofler, H. (2001) Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue. Am. J. Pathol. 158, 419-429.
50. Fink, L., Kinfe, T., Stein, M. M., Ermert, L., Hanze, J., Kummer, W., Seeger, W., and Bohle, R. M. (2000) Immunostaining and laser-assisted cell picking for mRNA analysis. Lab. Invest. 80, 327-333.
51. Fink, L., Kinfe, T., Seeger, W., Ermert, L., Kummer, W., and Bohle, R. M. (2000) Immunostaining for cell p[icking and real-time mRNA quantification. Am. J. Pathol. 157, 1459-1466.