Real-time quantitative RT-PCR after laser-assisted cell picking

Nature Medicine

Volumn 4, Issue 11, 1998, Pages 1329-1333

  Fink, Ludger ^a   Seeger, Werner ^a   Ermert, Leander ^a   Hänze, Jörg ^a   Stahl, Ulrich ^a   Grimminger, Friedrich ^a   Kummer, Wolfgang ^a   Bohle, Rainer M ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL SELECTION; CONTROLLED STUDY; GENE EXPRESSION; GENE TARGETING; ISOLATED LUNG; LASER; LUNG ALVEOLUS MACROPHAGE; LUNG LAVAGE; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALIA;

EID: 0031730893     PISSN: 10788956     EISSN: None     Source Type: Journal    
DOI: 10.1038/3327     Document Type: Article

References (16)

1. Holland, P.M., Abramson, R.D., Watson, R. & Gelfand, D.H. Detection of specific polymerase chain reaction product by utilizing the 59-39exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88, 7276-7280 (1991).
2. Lee, L.G., Connell, C.R. & Bloch, W. Allelic discrimination by nick-translation PCR with fluorogenic probes. Nucleic Acids Res. 21, 3761-3766 (1993).
3. Livak, K.J., Flood, S.J., Marmaro, J., Giusti, W. & Deetz, K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Applic. 4, 357-362 (1995).
4. Heid, C.A., Stevens, J., Livak, K.J. & Williams P.M. Real-time quantitative PCR. Genome Res. 6, 986-994 (1996).
5. Kueppers, R., Zhao, M., Hansmann, M.L. & Rajewsky, K. Tracing B-cell deveopment in human germinal centres by molecular analysis of single cells picked from histological sections. EMBO J. 12, 4955-4967 (1993).
6. Emmert-Buck, M.R. et al. Laser capture microdissection. Science 274, 998-1001 (1996).
7. Becker, I., Becker, K.F., Röhrl, M.H. & Höfler, H. Laser-assisted preparation of single cells from stained histological slides for gene analysis. Histochem. Cell Biol. 108, 447-451 (1997).
8. Kummer, W., Fink, L., Dvorakova, M., Haberberger, R. & Bohle, R.M. Rat cardiac neurons express the non-coding R-exon (exon-1) of the cholinergic gene locus. NeuroReport 9, 2209-2212 (1998).
9. Koerner, T.J., Adams, D.O. & Hamilton, T.A. Regulation of tumor necrosis factor (TNF) expression: Interferon-gamma enhances the accumulation of mRNA for TNF induced by lipopolysaccharide in murine peritoneal macrophages. Cell. Immunol. 109, 437-443 (1987).
10. Gibson, U.E.M., Heid, C.A. & Williams, P.M. A novel method for real-time quantitative RT-PCR. Genome Res. 6, 995-1001 (1996).
11. User Bulletin #2, ABI Prism 7700 Sequence Detection System. The Perkin-Elmer Corporation, P/N 4303859 Rev. A, Stock No. 777802-001 (1997).
12. Enser J.E., Crawford, E.K. & Hasday J.D. Warming macrophages to febrile range destabilizes tumor necrosis factor-alpha mRNA without inducing heat shock. Am. J. Physiol. 269, C1140-C1146 (1995).
13. Oliver J.C. et al. Cytokine kinetics in an in vitro whole blood model following an endotoxin challenge. Lymphokine Cytokine Res. 12, 115-120 (1993).
14. Seeger, W. et al. Adult respiratory distress syndrome: A model system using isolated perfused rabbit lungs. Meth. Enzymol. 233, 549-584 (1994).
15. Ermert, L. et al. Vasoregulatory prostanoid generation proceeds via cyclo-oxygenase-2 in non-inflamed rat lungs. J. Pharmacol. Exp. Therap. (in press).
16. Brady, G. & Iscove, N.N. Construction of cDNA libraries from single cells. Meth. Enzymol. 225, 611-623 (1993).