Analysis of anterior pituitary hormone mRNA expression in immunophenotypically characterized single cells after laser capture microdissection

Laboratory Investigation

Volumn 79, Issue 4, 1999, Pages 511-512

  Jin, Long ^a   Thompson, Corey A ^a   Qian, Xiang ^a   Kuecker, Sara J ^a   Kulig, Elzbieta ^a   Lloyd, Ricardo V ^a  

^a MAYO CLINIC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOHYPOPHYSIS HORMONE; MESSENGER RNA;

ANIMAL CELL; ARTICLE; CELL HETEROGENEITY; CELL LABELING; CELL STRUCTURE; CHROMOSOME MICRODISSECTION; GENE AMPLIFICATION; GENE EXPRESSION; IMMUNOPHENOTYPING; LASER; NONHUMAN; PRIORITY JOURNAL; PROLACTIN SECRETING CELL; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; DISSECTION; IMMUNOPHENOTYPING; LASERS; PITUITARY GLAND, ANTERIOR; PRO-OPIOMELANOCORTIN; PROLACTIN; RATS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

EID: 0032954501     PISSN: 00236837     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (7)

1. Bonner RF, Emmert-Buck M, Cole K, Pohida T, Chuaqui R, Goldstein S, and Liotta LA (1997). Laser capture microdissection: Molecular analysis of tissue. Science 278:1482-1483.
2. Drouin J, Chamberland M, Charron J, Jeannotte L, and Nemer M (1985). Structure of the rat pro-opiomelanocortin (POMC) gene. FEBS Lett 193:54-58.
3. Emmert-Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhuang Z, Goldstein SR, Weiss RA, and Liotta LA (1996). Laser capture microdissection. Science 274:998-1001.
4. Gubbins EJ, Maurer RA, Lagrimini M, Erwin CR, and Donelson JE (1980). Structure of the rat prolactin gene. J Biol Chem 255:8655-8662.
5. Konecki DS, Brennand J, Fuscoe JC, Caskey CT, and Chinault AC (1982). Hypoxanthine-guanine phosphoribosyl transferase genes of mouse and Chinese hamster: Construction and sequence analysis of cDNA recombinants. Nucleic Acids Res 10:6763-6775.
6. Qian X, Jin L, Grande JP, and Lloyd RV (1996). Transforming growth factor-β and p27 expression in pituitary cells. Endocrinology 137:3051-3060.
7. Schutze K and Lahr G (1998). Identification of expressed genes by laser-mediated manipulation of single cells. Nature Biotech 16:737-742.