Cell type-specific mRNA quantitation in non-neoplastic tissues after laser-assisted cell picking

Pathobiology

Volumn 68, Issue 4-5, 2000, Pages 191-195

  Bohle, Rainer M ^a   Hartmann, Elena ^a   Kinfe, Thomas ^a   Ermert, Leander ^a   Seeger, Werner ^a   Fink, Ludger ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

Endotoxin priming; Laser assisted cell picking; Lipopolysaccharide; Microdissection; mRNA quantitation; Nitric oxide synthase II mRNA; Real time; RT PCR

Indexed keywords

ENDOTOXIN; GAMMA INTERFERON; LIPOPOLYSACCHARIDE; MESSENGER RNA; NITRIC OXIDE SYNTHASE; PORPHOBILINOGEN DEAMINASE;

ANIMAL TISSUE; CELL TYPE; CHROMOSOME MICRODISSECTION; CONFERENCE PAPER; CONTROLLED STUDY; CRYOTHERAPY; GENE EXPRESSION SYSTEM; HOUSEKEEPING GENE; LUNG ALVEOLUS WALL; MACROPHAGE; MALE; MONOCYTE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS;

ANIMALS; CELL SEPARATION; CRYOPRESERVATION; DISSECTION; ENDOTHELIUM, VASCULAR; FIXATIVES; FORMALDEHYDE; HYDROXYMETHYLBILANE SYNTHASE; LASERS; LUNG; MALE; NITRIC OXIDE SYNTHASE; NITRIC OXIDE SYNTHASE TYPE II; ORGAN SPECIFICITY; RATS; RATS, SPRAGUE-DAWLEY; RNA, MESSENGER; TISSUE FIXATION;

EID: 0034460871     PISSN: 10152008     EISSN: None     Source Type: Journal    
DOI: 10.1159/000055922     Document Type: Conference Paper

References (11)

1. Fink L, Seeger W, Ermert L, Hänze J, Stahl U, Grimminger F, Kummer W, Bohle RM: Real-time quantitative RT-PCR after laser-assisted cell picking. Nat Med 1998;4:1329-1333.
2. Fink L, Stahl U, Ermert L, Kummer W, Seeger W, Bohle RM: Rat porphobilinogen deaminase: A pseudogene-free internal standard for laser-assisted cell picking. Biotechniques 1999; 26:510-516.
3. Ermert L, Ermert M, Althoff A, Merkle M, Grimminger F, Seeger W: Vasoregulatory prostanoid generation proceeds via cyclo-oxygenase-2 in non-inflamed rat lungs. J Pharmacol Exp Ther 1998;286:1309-1314.
4. Seeger W, Walmrath D, Grimminger F, Rosseau S, Schutte H, Kramer HJ, Ermert L, Kiss L: Adult respiratory distress syndrome: Model systems using isolated perfused rabbit lungs. Methods Enzymol 1994;233:549-584.
5. Fink L, Kinfe T, Stein MM, Ermert L, Hänze J, Kummer W, Seeger W, Bohle RM: Immunostaining and cell picking for mRNA analysis. Lab Invest 2000; 80:327-333.
6. Kummer W, Fink L, Dvorakova M, Haberberger R, Bohle RM: Rat cardiac neurons express the non-coding R-exon (exon 1) of the cholinergic gene locus. Neuroreport 1998;9: 2209-2212.
7. Heid CA, Stevens J, Livak KJ, Williams PM: Real time quantitative PCR. Genome Res 1996;6:986-994.
8. Gibson UEM, Heid CA, Williams PM: A novel method for real time quantitative RT-PCR. Genome Res 1996;6:995-1001.
9. Fink L, Kinfe T, Seeger W, Ermert L, Kummer W, Bohle RM: Immunostaining for cell picking and real-time mRNA quantitation. Am J Pathol 2000;157:1459-1466.
10. Ermert M, Merkle M, Mootz R, Grimminger F, Seeger W, Ermert L: Endotoxin priming of the cyclooxygenase-2-thromboxane axis in isolated rat lungs. Am J Physiol 2000;278:L1195-L1203.
11. Luo L, Salunga RC, Guo H, Bittner A, Joy KC, Galindo JE, Xiao H, Rogers KE, Wan JS, Jackson MR, Erlander MG: Gene expression profiles of laser-captured adjacent neuronal subtypes. Nat Med 1999;5:117-122.