Hic-5 interacts with GIT1 with a different binding mode from paxillin

Journal of Biochemistry

Volumn 132, Issue 2, 2002, Pages 279-289

  Nishiya, Naoyuki ^a   Shirai, Takeshi ^a   Suzuki, Wataru ^a   Nose, Kiyoshi ^a  

^a SHOWA UNIVERSITY   (Japan)

Author keywords

Forcal adhesion; GIT1; Hic 5; Paxillin

Indexed keywords

BINDING PROTEIN; GUANINE NUCLEOTIDE EXCHANGE FACTOR; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; PAXILLIN; PAXILLIN BINDING PROTEIN; PROTEIN GIT1; PROTEIN HIC5; UNCLASSIFIED DRUG; CELL CYCLE PROTEIN; CYTOSKELETON PROTEIN; DNA BINDING PROTEIN; GIT1 PROTEIN, HUMAN; GIT1 PROTEIN, MOUSE; HYBRID PROTEIN; PHOSPHOPROTEIN; PXN PROTEIN, HUMAN; PXN PROTEIN, MOUSE; RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 6; SIGNAL PEPTIDE; SIGNAL TRANSDUCING ADAPTOR PROTEIN; TGFB1I1 PROTEIN, HUMAN; TGFB1I1 PROTEIN, MOUSE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CELL ADHESION; CELL SPREADING; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME REGULATION; MOLECULAR DYNAMICS; MOUSE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN LOCALIZATION; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; TWO HYBRID SYSTEM; ANIMAL; BINDING SITE; CELL STRAIN 3T3; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE; SEQUENCE ALIGNMENT;

ANIMALIA;

3T3 CELLS; ADAPTOR PROTEINS, SIGNAL TRANSDUCING; AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CELL CYCLE PROTEINS; CYTOSKELETAL PROTEINS; DNA-BINDING PROTEINS; GTPASE-ACTIVATING PROTEINS; GUANINE NUCLEOTIDE EXCHANGE FACTORS; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MICE; MOLECULAR SEQUENCE DATA; PAXILLIN; PHOSPHOPROTEINS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SIGNAL TRANSDUCTION; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 0036684982     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003222     Document Type: Article

References (59)

1. Integrins: Emerging paradigms of signal transduction
2. Focal adhesions, contractility, and signaling
3. Paxillin and focal adhesion signalling
4. pp125FAK-dependent tyrosine phosphorylation of paxillin creates a high-affmity binding site for Crk
5. Characterization of tyrosine phosphorylation of paxillin in vitro by focal adhesion kinase
6. C3G, a guanine nucleotide-releasing protein expressed ubiquitously, binds to the Src homology 3 domains of CRK and GRB2/ASH proteins
7. DOCK180, a major CRK-binding protein, alters cell morphology upon translocation to the cell membrane
8. Identification of LIM3 as the principal determinant of paxillin focal adhesion localization and characterization of a novel motif on paxillin directing vinculin and focal adhesion kinase binding
9. Paxillin LD motifs may define a new family of protein recognition domains
10. Primary sequence of paxillin contains putative SH2 and SH3 domain binding motifs and multiple LIM domains: Identification of a vinculin and pp125Fak-binding region
11. Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling
12. The LD4 motif of paxillin regulates cell spreading and motility through an interaction with paxillin kinase linker (PKL)
13. Characterization of the TGF beta 1-inducible hic-5 gene that encodes a putative novel zinc finger protein and its possible involvement in cellular senescence
14. Induction of senescence-like phenotypes by forced expression of hic-5, which encodes a novel LIM motif protein, in immortalized human fibroblasts
15. Forced expression of hic-5, a senescence-related gene, potentiates a differentiation process of RCT-1 cells induced by retinoic acid
16. Characterization of a focal adhesion protein, Hic-5, that shares extensive homology with paxillin
17. Hic-5, a paxillin homologue, binds to the protein-tyrosine phosphatase PEST (PTP-PEST) through its LIM 3 domain
18. Hic-5-reduced cell spreading on fibronectin: Competitive effects between paxillin and Hic-5 through interaction with focal adhesion kinase
19. Interaction of Hic-5, A senescencerelated protein, with focal adhesion kinase
20. Actin-based cell motility and cell locomotion
21. Rho GTPases and the actin cytoskeleton
22. Rho: Theme and variations
23. Activation of Rac and Cdc42 by integrins mediates cell spreading
24. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia
25. Rho GTPases and their effector proteins
26. A brain serine/threonine protein kinase activated by Cdc42 and Racl
27. PAK promotes morphological changes by acting upstream of Rac
28. A conserved negative regulatory region in alpha-PAK: Inhibition of PAK kinases reveals their morphological roles downstream of Cdc42 and Racl
29. p21-activated kinase 1 (Pak1) regulates cell motility in mammalian fibroblasts
30. A role for p21-activated kinase in endothelial cell migration
31. A novel regulator of p21-activated kinases
32. PAK kinases are directly coupled to the PIX family of nucleotide exchange factors
33. alphaPIX nucleotide exchange factor is activated by interaction with phosphatidylinositol 3-kinase
34. beta2-Adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein
35. The GIT family of ADP-ribosylation factor GTPase-activating proteins. Functional diversity of GIT2 through alternative splicing
36. p95-APP1 links membrane transport to Rac-mediated reorganization of actin
37. ARF6 requirement for Rac ruffling suggests a role for membrane trafficking in cortical actin rearrangements
38. ADP-ribosylation factor 6 regulates actin cytoskeleton remodeling in coordination with Rac1 and RhoA
39. Tax protein of human T-cell leukemia virus type I binds to the ankyrin motifs of inhibitory factor kappa B and induces nuclear translocation of transcription factor NF-kappa B proteins for transcriptional activation
40. The LIM domains of hic-5 protein recognize specific DNA fragments in a zinc-dependent manner in vitro
41. The two-hybrid system: A method to identify and clone genes for proteins that interact with a protein of interest
42. Cell adhesion kinase beta forms a complex with a new member, Hic-5, of proteins localized at focal adhesions
43. Specific decrease in the level of Hic-5, a focal adhesion protein, during immortalization of mouse embryonic fibroblasts, and its association with focal adhesion kinase
44. Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex disassembly
45. Direct association of pp125FAK with paxillin, the focal adhesion-targeting mechanism of pp125FAK
46. The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton
47. A new paxillin-binding protein, PAG3/Papalpha/KIAA0400, bearing an ADP-ribosylation factor GTPase-activating protein activity, is involved in paxillin recruitment to focal adhesions and cell migration
48. The bovine papillomavirus E6 protein binds to the LD motif repeats of paxillin and blocks its interaction with vinculin and the focal adhesion kinase
49. Association of Bovine Papillomavirus Type 1 E6 oncoprotein with the focal adhesion protein paxillin through a conserved protein interaction motif
50. Characterisation of the paxillin-binding site and the C-terminal focal adhesion targeting sequence in vinculin
51. Tyrosine kinase activity, cytoskeletal organization, and motility in human vascular endothelial cells
52. Activation of protein kinase C precedes alpha 5 beta 1 integrin-mediated cell spreading on fibronectin
53. Inhibition of cell spreading by expression of the C-terminal domain of focal adhesion kinase (FAK) is rescued by coexpression of Src or catalytically inactive FAK: A role for paxillin tyrosine phosphorylation
54. Phosphorylation of tyrosine residues 31 and 118 on paxillin regulates cell migration through an association with CRK in NBT-II cells
55. Paxillin alpha and Crk-associated substrate exert opposing effects on cell migration and contact inhibition of growth through tyrosine phosphorylation
56. Inhibition of myosin light chain kinase by p21-activated kinase
57. Polarized distribution of endogenous Rac1 and RhoA at the cell surface
58. Localized Rac activation dynamics visualized in living cells
59. Separation of membrane trafficking and actin remodeling functions of ARF6 with an effector domain mutant