Inhibition of cell spreading by expression of the C-terminal domain of focal adhesion kinase (FAK) is rescued by coexpression of Src or catalytically inactive FAK: A role for paxillin tyrosine phosphorylation

Molecular and Cellular Biology

Volumn 17, Issue 12, 1997, Pages 6906-6914

  Richardson, Alan ^a,c   Malik, Rajesh K ^b   Hildebrand, Jeffrey D ^a,d   Parsons, J Thomas ^a  

^a UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

^c JANSSEN RESEARCH FOUNDATION   (Belgium)

^d Fred Hutchinson Cancer Research Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FOCAL ADHESION KINASE; PROTEIN TYROSINE KINASE;

ANIMAL CELL; ARTICLE; AUTOPHOSPHORYLATION; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CELL ADHESION; CELL MOTILITY; CELL SPREADING; EXTRACELLULAR MATRIX; GENE EXPRESSION; GENE OVEREXPRESSION; NONHUMAN; ONCOGENE SRC; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN PHOSPHORYLATION;

ANIMALIA;

EID: 0030694182     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.17.12.6906     Document Type: Article

References (49)

1. FAK) expression correlates with motility of human melanoma cell lines. J. Invest. Dermatol. 105:104-108.
2. Bachelot, C., L. Rameh, T. Parsons, and L. C. Cantley. 1996. Association of phosphatidylinositol 3-kinase, via the SH2 domains of p85, with focal adhesion kinase in polyoma middle T-transformcd fibroblasts. Biochim. Biophys. Acta 1311:45-52.
3. 5 integrin, and interferes with HeLa cell spreading. Mol. Cell. Biol. 15:711-722.
4. Bretscher, M. S. 1996. Getting membrane flow and the cytoskeleton to cooperate in moving cells. Cell 87:601-606.
5. Burridge, K., and M. Chrzanowska-Wodnicka. 1996. Focal adhesions, contractility and signaling. Annu. Rev. Cell Dev. Biol. 12:463-519.
6. Burridge, K., K. Fath, T. Kelly, G. Nuckolls, and C. Turner. 1988. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton. Annu. Rev. Cell Biol. 4:487-525.
7. Calalb, M. B., T. R. Polte, and S. K. Hanks. 1995. Tyrosine phosphorylation of focal adhesion kinase at sites in the catalytic domain regulates kinase activity: a role for Src family kinases. Mol. Cell. Biol. 15:954-963.
8. Carey, L. A., J. F. Chang, and J.-L. Guan. 1996. Stimulation of cell migration by overexpression of focal adhesion kinase and its association with Src and Fyn. J. Cell. Sci. 109:1787-1794.
9. Chen, H.-C., P. A. Appeddu, H. Isoda, and J.-L. Guan. 1996. Phosphorylation of tyrosine 397 in focal adhesion kinase is required for binding phosphatidylinositol 3-kinase. J. Biol. Chem. 271:26329-26334.
10. Chen, H.-C., P. A. Appeddu, J. T. Parsons, J. D. Hildebrand, M. D. Schaller, and J.-L. Guan. 1995. Interaction of focal adhesion kinase with cytoskeletal protein talin. J. Biol. Chem. 270:16995-16999.
11. Chen, H.-C., and J.-L. Guan. 1994. Association of focal adhesion kinase with its potential substrate phosphatidylinositol 3-kinase. Proc. Natl. Acad. Sci. USA 91:10148-10152.
12. FAK. Mol. Cell. Biol. 14:147-155.
13. Gilmore, A. P., and L. H. Romer. 1996. Inhibition of focal adhesion kinase (FAK) signaling in focal adhesions decreases cell motility and proliferation. Mol. Biol. Cell 7:1209-1224.
14. Guinebault, C., B. Payrastre, C. Racaaud-Sultan, H. Mazarguil, M. Breton, G. Mauco, M. Plantavid, and H. Chap. 1995. Integrin-dependent translocation of phosphoinositide 3-kinase to the cytoskeleton of thrombin-activated platelets involves specific interactions of p85-alpha with actin filaments and focal adhesion kinase. J. Cell Biol. 129:831-842.
15. Hanks, S. K., M. B. Calalb, M. C. Harper, and S. K. Patel. 1992. Focal adhesion protein-tyrosine kinase phosphorylated in response to cell attachment to fibronectin. Proc. Natl. Acad. Sci. USA 89:8487-8491.
16. Cas, a substrate associated with v-Src and v-Crk, localizes to focal adhesions and binds to focal adhesion kinase. J. Biol. Chem. 271: 13649-13655.
17. FAK, to cellular focal adhesions. J. Cell Biol. 123:993-1005.
18. Hildebrand, J. D., M. D. Schaller, and J. T. Parsons. 1995. Paxillin, a tyrosine phosphorylated focal adhesion-associated protein, binds to the carboxyl terminal domain of focal adhesion kinase. Mol. Biol. Cell 6:637-647.
19. Hildebrand, J. D., J. M. Taylor, and J. T. Parsons. 1996. An SH3 domain-containing GTPase-activating protein for Rho and Cdc42 associates with focal adhesion kinase. Mol. Cell. Biol. 16:3169-3178.
20. Huttenlocher, A., R. Sandborg, and A. F. Horwitz. 1995. Adhesion in cell migration. Curr. Biol. 7:697-706.
21. Hynes, R. O. 1992. Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69:11-25.
22. Ilic, D., S. Kanazawa, Y. Furuta, T. Yamamoto, and S. Aizawa. 1996. Impairment of mobility in endodermal cells by FAK deficiency. Exp. Cell Res. 222:298-303.
23. Ilic, D., Y. Furuta, S. Kanazawa, N. Takeda, K. Sobue, N. Nakatsu, S. Nomura, J. Fujimoto, M. Okada, T. Yamamoto, and S. Aizawa. 1995. Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice. Nature 377:539-543.
24. Kanner, S. B., A. B. Reynolds, R. R. Vines, and J. T. Parsons. 1990. Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases. Proc. Natl. Acad. Sci. USA 87:3328-3332.
25. Kaplan, K. B., J. R. Swedlow, D. O. Morgan, and H. E. Varmus. 1995. c-Src enhances the spreading of sic-/-fibroblasts on fibronectin by a kinase-independent mechanism. Genes Dev. 9:1505-1517.
26. Lauffenburger, D. A., and A. F. Horwitz. 1996. Cell migration: a physically integrated molecular process. Cell 84:359-369.
27. FAK in platelets. J. Cell Biol. 119:905-912.
28. Mitchison, T. J., and L. P. Cramer. 1996. Actin-based cell motility and cell locomotion. Cell 84:371-379.
29. Miyamoto, S., S. K. Akiyama, and K. M. Yamada. 1995. Synergistic roles for receptor occupancy and aggregation in integrin transmembrane function. Science 267:883-885.
30. Miyamoto, S., H. Teramoto, O. A. Coso, J. S. Gutkind, P. D. Burbelo, S. K. Akiyama, and K. M. Yamada. 1995. Integrin function: molecular hierarchies of cytoskeletal and signaling molecules. J. Cell Biol. 131:791-805.
31. Nobes, C. D., and A. Hall. 1995. Rho, Rac. and Cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell 81:53-62.
32. FAK) in invasive human tumors. Cancer Res. 55:2752-2755.
33. Palecek, S. P., J. C. Loftus, M. H. Ginsberg, D. A. Lauffenburger, and A. F. Horwitz. 1997. Integrin-ligand binding properties govern cell migration speed through cell-substratum adhesiveness. Nature 385:537-540.
34. Cas. Proc. Natl. Acad. Sci. USA 92:10678-10682.
35. Cas) are elevated in cytoskeleton-associated fractions following adhesion and Src transformation. J. Biol. Chem. 272:5501-5509.
36. Reynolds, A. B., D. J. Roesel, S. B. Kanner, and J. T. Parsons. 1989. Transformation-specific tyrosine phosphorylation of a novel cellular protein in chicken cells expressing oncogenic variants of the avian cellular src gene. Mol. Cell. Biol. 9:629-638.
37. Richardson, A., and J. T. Parsons. 1995. Signal transduction through integrins: a central role for focal adhesion kinase? Bioessays 17:229-236.
38. FAK. Nature 380:538-540.
39. Sabe, H., A. Hata, M. Okada, H. Nakagawa, and H. Hanafusa. 1994. Analysis of the binding of the Src homology 2 domain of Csk to tyrosine phosphorylated proteins in the suppression and mitotic activation of c-Src. Proc. Natl. Acad. Sci. USA 91:3984-3988.
40. FAK, a structurally distinctive protein-tyrosine kinase associated with focal adhesions. Proc. Natl. Acad. Sci. USA 89:5192-5196.
41. FAK. Mol. Cell. Biol. 13:785-791.
42. src. Mol. Cell. Biol. 14: 1680-1688.
43. Schaller, M. D., C. A. Otey, J. D. Hildebrand, and J. T. Parsons. 1995. Focal adhesion kinase and paxillin bind to peptides mimicking beta integrin cytoplasmic domains. J. Cell Biol. 130:1181-1187.
44. Schaller, M. D., and J. T. Parsons. 1994. Focal adhesion kinase and associated proteins. Curr. Opin. Cell Biol. 6:705-710.
45. FAK-dependent tyrosine phosphorylation of paxillin creates a high-affinity binding site for Crk. Mol. Cell. Biol. 15:2635-2645.
46. Schlaepfer, D. D., S. K. Hanks, T. Hunter, and P. van der Geer. 1994. Integrin-mediated signal transduction linked to Ras pathway by GRB2 binding to focal adhesion kinase. Nature 372:786-791.
47. Schlaepfer, D. D., and T. Hunter. 1996. Evidence for in vivo phosphorylation of the Grb2 SH2-domain binding site on focal adhesion kinase by Src-family protein-tyrosine kinases. Mol. Cell. Biol. 16:5623-5633.
48. Thomas, S. M., P. Soriano, and A. Imamoto. 1995. Specific and redundant roles of Src and Fyn in organizing the cytoskeleton. Nature 376:267-271.
49. Weiner, T. M., E. T. Liu, R. J. Craven, and W. G. Cance. 1993. Expression of focal adhesion kinase gene in invasive cancer. Lancet 342:1024-1025.