Identification of LIM3 as the principal determinant of paxillin focal adhesion localization and characterization of a novel motif on paxillin directing vinculin and focal adhesion kinase binding

Journal of Cell Biology

Volumn 135, Issue 4, 1996, Pages 1109-1123

  Brown, Michael C ^a   Perrotta, Joseph A ^a   Turner, Christopher E ^a  

^a SUNY UPSTATE MEDICAL UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOSKELETON PROTEIN; GLUTAMIC ACID; GLUTAMINE; HOMEODOMAIN PROTEIN; PHOSPHOPROTEIN; PROTEIN KINASE P60; PROTEIN TYROSINE KINASE; TRANSCRIPTION FACTOR; TRANSFORMING PROTEIN; VINCULIN; ZINC FINGER PROTEIN;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CELL ADHESION; CELLULAR DISTRIBUTION; CHO CELL; CONTROLLED STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN TARGETING; SITE DIRECTED MUTAGENESIS;

ANIMALIA;

EID: 0029827130     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.135.4.1109     Document Type: Article

References (55)

1. Altschul, S.F., W. Gish, W. Miller, E.W. Myers, and D.J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Arber, S., and P. Caroni. 1996. Specificity of single LIM motifs in targeting and LIM/LIM interactions in situ. Genes & Dev. 10:289-300.
3. Avraham, S., R. London, Y. Fu, S. Ota, D. Hiregowdara, J. Li, S. Jiang, L.M. Pasztor, R.A. White, J.E. Groopman, and H. Avraham. 1995. Identification and characterization of a novel related adhesion focal tyrosine kinase (RAFTK) from megakaryocytes and brain. J. Biol. Chem. 270:27742-27751.
4. Backer, J.M., S.E. Shoelson, M.A. Weiss, Q.X. Hua, R.B. Cheatham, E. Haring, D.C. Cahill, and M.F. White. 1992. The insulin receptor juxtamembrane region contains two independent tyrosine/β-turn internalization signals. J. Cell Biol. 118:831-839.
5. Belkin, A.M., O.I. Ornatsky, M.A. Glukhora, and V.E. Koteliansky. 1988. Immunolocalization of meta-vinculin in human smooth and cardiac muscles. J. Cell Biol. 107:545-553.
6. Bellis, S.L., J.T. Miller, and C.E. Turner. 1995. Characterization of tyrosine phosphorylation of paxillin in vitro by focal adhesion kinase. J. Biol. Chem. 270:17437-17441.
7. Birge, R.B., J.E. Fajardo, C. Reichman, S.E. Shoelson, Z. Songyang, L.C. Cantley, and H. Hanafusa. 1993. Identification and characterization of a high affinity interaction between v-crk and tyrosine-phosphorylated paxillin in CT10-transformed fibroblasts. Mol. Cell. Biol. 13:4648-4656.
8. Burridge, K. 1986. Substrate adhesions in normal and transformed fibroblasts: organization and regulation of cytoskeletal, membrane and extracellular matrix components at focal contacts. Cancer Rev. 4:18-78.
9. Burridge, K., K. Fath, T. Kelly, G. Nuckolls, and C. Turner. 1988. Focal adhesions: Transmembrane junctions between (he extracellular matrix and the cytoskeleton. Annu. Rev. Cell Biol. 4:487-525.
10. FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly. J. Cell Biol. 119:893-903.
11. Chen, W.-J., J.L. Goldstein, and M.S. Brown. 1990. NPXY. a sequence often found in cytoplasmic domains, is required for coated pit-mediated internalization of the low density lipoprotein receptor. J. Biol. Chem. 265:3116-3123.
12. Clark, J.A., and J.S. Brugge. 1995. Integrins and signal transduction pathways: the road taken. Science (Wash. DC). 268:233-239.
13. Coll, J.-L, A. Ben-Ze'ev, R.M. Ezzell, J.L. Rodríguez Fernández, H. Baribault, R.G. Oshima, and E.D. Adamson. 1995. Targeted disruption of vinculin genes in F9 and embryonic stem cells changes cell morphology, adhesion, and locomotion. Proc. NaIl. Acad. Sci. USA. 92:9161-9165.
14. Crawford, A.W., J.W. Michelsen, and M.C. Beckerle. 1992. An interaction between zyxin α-actinin. J. Cell Biol. 116:1381-1393.
15. Fernandez, J.L., B. Geiger, D. Salomon, and A. Ben-Ze'ev. 1993. Suppression of vinculin expression by antisense transfection confers changes in cell morphology, motility, and anchorage-dependent growth of 3T3 cells. J. Cell Biol. 122:1285-1294.
16. Feuerstein, R., X. Wang, D. Song, N.E. Cooke, and S.A. Liebhaber. 1994. The LIM/double zinc-finger motif functions as a protein dimerization domain. Proc. Natl. Acad. Sci. USA. 91:10655-10659.
17. Guan, J.-L., and D. Shalloway. 1992. Regulation of focal adhesion-associated protein tyrosine kinase by both cellular adhesion and oncogenic transformation. Nature (Lond). 358:690-692.
18. Hanks, S.K., M.B. Calalb, M.C. Harper, and S.K. Patel. 1992. Focal adhesion-protein kinase phosphorylated in response to cell attachment to fibronectin. Proc. Natl. Acad. Sci. USA. 89:8487-8491.
19. Hemmings, L., S.T. Barry, and D.R. Critchley. 1995. Cell-matrix adhesion: structure and regulation. Biochem. Soc. Trans. 23:619-626.
20. Hildebrand, J.D., M.D. Schaller, and J.T. Parsons. 1995. Paxillin, a tyrosine phosphorylated focal adhesion-associated protein binds to the carboxyl terminal domain of focal adhesion kinase. Mol. Biol. Cell. 6:637-647.
21. Hynes, R.O. 1992 Integrin: versatility, modulation, and signaling in cell adhesion. Cell. 69:11-25.
22. Ilic, D., Y. Furuta, S. Kanazawa, N. Takeda, K. Sobue, N. Nakatsuji, S. Nomura, J. Fujimoto, M. Okada, T. Yamamoto, and S. Alzawa. 1995. Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice. Nature (Lond.). 377:539-544.
23. Juliano, R.L., and S. Haskill. 1993. Signal transduction from the extracellular matrix. J. Cell Biol. 120:577-585.
24. Kornberg, L., H.S. Earp, J.T. Parsons, M. Schaller, and R.L. Juliano. 1992. Cell adhesion or integrin clustering increases phosphorylation of a focal adhesion-associated tyrosine kinase. J. Biol. Chem. 267:23439-23442.
25. Kunkel, T.A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Prof. Natl. Acad. Sci. USA. 82:488-492.
26. Lev, S., H. Moreno, R. Martinez, P. Canoll, E. Peles, J.M. Musacchio, G.D. Plowman, B. Rudy, and J. Schlessinger. 1995. Protein tyrosine kinase PYK2 involved in Ca'*-induced regulation of ion channel and MAP kinase func-tions. Nature (Lond.). 376:737-745.
27. Li, J., H. Avraham, R.A. Rogers. S. Raja, and S. Avraham. 1996. Characterization of RAFTK, a novel focal adhesion kinase, and its integrin-dependent phosphorylation and activation in megakaryocytes. Blood. 88:417-428.
28. Lo, S.H., and L.B. Chen. 1994. Focal adhesion as a signal transduction organelle. Cancer Metastasis Rev. 13:9-24.
29. Luna, E.J., and A.L. Hitt. 1992. Cytoskeleton-plasma membrane interactions. Science (Wash. DC). 258:955-964.
30. Miyamoto, S., J. Teramoto, O.A. Coso, J.S. Gutkind, P.D. Burbelo, S.K. Akiyama, and K.M. Yamada. 1995. Integrin function: molecular hierarchies of cytoskeletal and signaling molecules. J. Cell Biol. 131:791-805.
31. Okano, I., J. Hiraoki, H. Otera, K. Ninoue, K. Ohashi, S. Iwashita, M. Hirai, and K. Mizuno. 1995. Identification and characterization of a novel family of serine/threonine kinase containing N-terminal LIM motifs J. Biol. Chem. 270:31321-31330.
32. O'Toole, T.E., J. Ylanne, and B.M. Culley. 1995. Regulation of integrin affinity states through an NPXY motif in the β subunit cytoplasmic domain. J. Biol. Chem. 270:8553-8558.
33. Rabbitts,T.H., and T. Boehm. 1990. LIM domains. Nature (Lond.). 346:418.
34. Ren, R., B.J. Mayer, P. Cichetti, and D. Baltimore. 1993. Identification of a tenamino acid proline-rich SH3 binding site. Science (Wash. DC). 259:1157-1161.
35. Reszka, A.A., Y. Hayashi, and A.F. Horwitz. 1992. Identification of ammo acid sequences in the integrin β1 cytoplasmic domain implicated in cytoskeletal association. J. Cell Biol. 117:1321-1330.
36. Sadler, I., A.W. Crawford, J.W. Michelsen, and M.C. Beckerle. 1992. Zyxin and cCRP: two interactive LIM domain proteins asscociated with the cytoskeleton. J. Cell Biol. 119:1573-1587.
37. Samuels, M., R.M. Ezzell. T.J. Cardozo, D.R. Critchley, J.-L. Coll. and E.D. Adamson. 1993. Expression of chicken vinculin complements the adhesion-defective phenotype of a mutant mouse F9 embryonal carcinoma cell. J. Cell Biol. 121:909-921.
38. Sánchez-García, I., and T.H. Rabbitts. 1994. The LIM domain: a new structural motif found in zinc-finger-like proteins. Trends Genet. 10:315-320.
39. Sasaki, H., K. Nagura, M. Ishino, H. Tobioka, K. Kotani, and T. Sasaki. 1995. Cloning and characterization of cell adhesion kinase β, a novel protein-tyrosine kinase of the focal adhesion kinase subfamily. J. Biol. Chem. 270: 21206-21219.
40. FAK-dependent tyrosine phosphorylation of paxillin creates a high-affinity binding site for crk. Mol. Cell. Biol. 15:2635-2645.
41. FAK. Mol. Cell. Biol. 13:785-791.
42. Schaller, M.D., C.A. Otey, J.D. Hildebrand, and J.T. Parsons. 1995. Focal adhesion kinase and paxillin bind to peptides mimicking β integrin cytoplasmic domains. J. Cell Biol. 130:1181-1187.
43. Shibanuma, M., J.-I Mashimo, T. Kuroki, and K. Nose. 1994. Characterization of the TGFβ1-inducible hic-5 gene that encodes a putative novel zinc finger protein and its possible involvement in cellular senescence. J. Biol. Chem. 269:26767-26774.
44. FAK, J. Exp Med 182:1089-1100.
45. Towbin, H., T. Staehlin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:4350-4354.
46. Turner, C.E. 1994. Paxillin: a cytoskeletal target for tyrosine kinases. Bioessays. 16:47-52.
47. Turner, C.E., and K. Burridge. 1990. Transmembrane molecular assemblies in cell-extracellular matrix interactions. Clin. Opin. Cell Biol. 3:849-853.
48. FAK binding region. J. Cell Sci. 107:1583-1591.
49. Turner, C.E., J.R. Glenney, and K. Burridge. 1990. Paxillin: a new vinculin-binding protein present in focal adhesions. J. Cell Biol. 111:1059-1068.
50. FAK during development: relation to paxillin. J. Cell Sci. 105:637-645.
51. Turner, C.E., K.M. Pietras, D.S. Taylor, and C.J. Molloy. 1995. Angiotensin II stimulation of rapid paxillin tyrosine phosphorylation correlates with the formation of focal adhesions in rat aortic smooth muscle cells. J. Cell Sci. 108:333-342.
52. Volberg, T., B. Geiger, Z. Kam, R. Pankov, I. Simcha, H. Sabanay, J.-L. Coll, E. Adamson, and A. Ben-Ze'ev. 1995. Focal adhesion formation by F9 embryonal carcinoma cells after vinculin gene disruption. J. Cell Sci. 108:2253-2260.
53. Weng, Z., J.A. Taylor, C.E. Turner, J.S. Brugge, and C. Seidel-Dugan. 1993. Detection of src-SH3 binding proteins, including paxillin, in normal and v-src-transformed BALBc/3T3 cells. J. Biol. Chem. 268:14956-14963.
54. Wood, C.K., C.E. Turner, P. Jackson, and D.R. Critchley. 1994. Characterisation of the paxillin-binding site and the C-terminal focal adhesion targeting sequence in vinculin. J. Cell Sci. 107:709-717.
55. Wu, R.-Y., and G.N. Gill. 1994. LIM domain recognition of a tyrosine-containing tight turn. J. Biol. Chem. 269:25085-25090