Inhibition of trypsin by cowpea thionin: Characterization, molecular modeling, and docking

Proteins: Structure, Function and Genetics

Volumn 48, Issue 2, 2002, Pages 311-319

  Melo, Francislete R ^a,b   Rigden, Daniel J ^b   Franco, Octávio L ^b,c   Mello, Luciane V ^b   Ary, Maria B ^d   Grossi De Sá, Maria F ^b   Bloch Jr , Carlos ^b  

^a UNIVERSITY OF BRASILIA   (Brazil)

^b EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA   (Brazil)

^c UNIVERSIDADE CATÓLICA DE BRASÍLIA   (Brazil)

^d FEDERAL UNIVERSITY OF CEARÁ   (Brazil)

Author keywords

Cowpea; Molecular modeling; Thionin; Trypsin inhibitor

Indexed keywords

LYSINE; PROTEINASE; THIONINE; TRYPSIN;

ARTICLE; COWPEA; MASS SPECTROMETRY; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; STOICHIOMETRY; X RAY CRYSTALLOGRAPHY;

EMBRYOPHYTA; VIGNA; VIGNA UNGUICULATA;

EID: 0036681427     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/prot.10142     Document Type: Article

References (67)

1. Antimicrobial peptides from plants
2. Thionins
3. Thionins: Properties, possible biological roles and mechanisms of action
4. The role of thionins in plant protection
5. Plant defense peptides
6. CATH - A hierarchic classification of protein domain structures
7. Differential effects of five types of antipathogenic plant peptides on model membranes
8. Fungal membrane responses induced by plant defensins and thionins
9. Wounding and chemicals induce expression of the Arabidopsis thaliana gene Thi2.1, encoding a fungal defense thionin, via the octadecanoid pathway
10. Selective disulphide linkage of plant thionins with other proteins
11. A new family of small (5 kDa) protein inhibitors of insect alpha-amylases from seeds or sorghum (Sorghum bicolor (L) Moench) have sequence homologies with wheat gamma-purothionins
12. Molecular cloning and analysis of four potato tuber mRNAs
13. α-amylase inhibitors from cowpea seeds
14. Alkylation and identification of the histidine residues at the active site of ribonuclease
15. The involvement of cysteine proteases and protease inhibitor genes in the regulation of programmed cell death in plants
16. Thermodynamics of the binding of chymotrypsin with the black-eyed pea trypsin and chymotrypsin inhibitor (BTCI)
17. Improved tools for biological sequence comparison
18. CAFASP-1: Critical assessment of fully automated structure prediction methods
19. GenTHREADER: An efficient and reliable protein fold recognition method for genomic sequences
20. Hybrid fold recognition: Combining sequence derived properties with evolutionary information
21. Hidden Markov models for detecting remote protein homologies
22. Solution structure of gamma 1-H and gamma 1-P thionins from barley and wheat endosperm determined by 1H-NMR: A structural motif common to toxic arthropod proteins
23. An automated approach for clustering an ensemble of NMR-derived protein structures into conformationally related subfamilies
24. Comparative protein modelling by satisfaction of spatial restraints
25. Evaluation of comparative protein structure modeling by MODELLER-3
26. Recognition of errors in three-dimensional structures of proteins
27. PROCHECK: A program to check stereochemical quality of protein structures
28. Prediction of protein conformational freedom from distance constraints
29. Conformational changes in the chaperonin GroEL: New insights into the allosteric mechanism
30. Essential dynamics of proteins
31. Collective motions in proteins: A covariance analysis of atomic fluctuations in molecular dynamics and normal mode simulations
32. Structural basis of the endoproteinase-protein inhibitor interaction
33. Conformational homogeneity in molecular recognition by proteolytic enzymes
34. Structure of the complex formed by bovine trypsin and bovine pancreatic trypsin inhibitor. Crystallographic refinement at 1.9 Angstroms
35. Crystal structure of an engineered subtilisin inhibitor complexed with bovine trypsin
36. The refined 2.0 Å X-ray crystal structure of the complex formed between bovine beta-trypsin and CMTI-I, a trypsin inhibitor from squash seeds (Cucurbita maxima). Topological similarity of the squash seed inhibitors with the carboxypeptidase A inhibitor from potatoes
37. Structure of the trypsin-binding domain of Bowman-Birk type protease inhibitor and its interaction with trypsin
38. Molecular surface recognition: Determination of geometric fit between proteins and their ligands by correlation techniques
39. The structure of a complex of recombinant hirudin and human alpha-thrombin
40. The ornithodorin-thrombin crystal structure, a key to the TAP enigma?
41. Rapid refinement of protein interfaces incorporating solvation: Application to the docking problem
42. Principles of protein-protein interactions derived from structural studies
43. Satisfying hydrogen bonding potential in proteins
44. The atomic structure of protein-protein recognition sites
45. Hydrophobic docking: A proposed enhancement to molecular recognition techniques
46. Modelling protein docking using shape complementarity, electrostatics and biochemical information
47. Electrostatic aspects of protein-protein interactions
48. Scoring docked conformations generated by rigid-body protein-protein docking
49. Examination of shape complementarity in docking of unbound proteins
50. H-NMR structure of an antifungal γ-thionin protein Siα1: Similarity to scorpion toxins
51. Defense proteins from seed of Cassia fistula include a lipid transfer protein homologue and a protease inhibitory plant defensin
52. The HSSP data base of protein structure-sequence alignments
53. Twilight zone of protein sequence alignments
54. BLAST 2 sequences, a new tool for comparing protein and nucleotide sequences
55. Annotating eukaryoted genomes
56. Representation of functional information in the SWISS-PROT data bank
57. Purification, biochemical characterization and partial primary structure of a new alpha-amylase inhibitor from Secale cereale (Rye)
58. Dynamic properties of the guanine nucleotide binding protein alpha subunit and comparison of its triphosphate hydrolase domain with that of ras p21
59. Mapping the protein universe
60. SCOP: A structural classification of proteins database for the investigation of sequences and structures
61. The geometry of the reactive site and of the peptide groups in trypsin, trypsinogen and its complexes with inhibitors
62. Identifying proteins with insecticidal activity: Use of encoding genes to produce insect-resistant transgenic crops
63. Insect-resistant transgenic plants
64. Tissue-specific and developmental regulation of a gene encoding a low molecular weight sulfurrich protein in soybean seeds
65. A defensin gene expressed in sunflower inflorescence
66. Complete amino acid sequences of two gamma-thionins from maize (Zea mays L.) seeds