A defensin gene expressed in sunflower inflorescence

Plant Physiology and Biochemistry

Volumn 38, Issue 3, 2000, Pages 253-258

  Urdangarín, María Carina ^a   Norero, Natalia Sigrid ^a   Broekaert, Willem F ^b   De La Canal, Laura ^a  

^a UNIVERSIDAD NACIONAL DE MAR DEL PLATA   (Argentina)

^b UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

Antimicrobial peptides; Defensin; Helianthus annuus; Innate resistance; Lipid transfer proteins; nt, nucleotide; ORF, open reading frame; Plant defense; RT PCR, reverse transcription polymerase chain reaction; Sclerotinia sclerotiorum; SD, sunflower defensin; Sunflower LTPs; UTR, untranslated region

Indexed keywords

HELIANTHUS ANNUUS;

EID: 0034115847     PISSN: 09819428     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0981-9428(00)00737-3     Document Type: Article

References (28)

1. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. Current Protocols in Molecular Biology. 1987;John Willey, New York.
2. Broekaert W.F., Cammue B.P.A., De Bolle M.F.C., Thevissen K., De Samblanx G.W., Osborn R.W. Antimicrobial peptides from plants. Crit. Rev. Plant Sci. 16:1997;297-323.
3. Broekaert W.F., Terras F.R.G., Cammue B.P.A., Osborn R.W. Plant defensins: novel antimicrobial peptides as components of the host defence system. Plant Physiol. 108:1995;1353-1358.
4. Carmona M.J., Molina A., Fernandez J.A., Lopez-Fando J.J., García-Olmedo F. Expression of the alpha-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens. Plant J. 3:1993;457-462.
5. Choi Y., Choi Y.D., Lee J.S. Nucleotide sequence of a cDNA encoding a low molecular weight sulfur-rich protein in soybean seeds. Plant Physiol. 101:1993;699-700.
6. De Samblanx G.W., Goderis I.J., Thevissen K., Raemaekers R., Fant F., Borremans F., Acland D.P., Osborn R.W., Patel S., Broekaert W.F. Mutational analysis of a plant defensin from radish (Raphanus sativus L.) reveals two adjacent sites important for antifungal activity. J. Biol. Chem. 272:1997;1171-1179.
7. Domon C., Evrard J.L., Herdenberger F., Pillay D.T.N., Steinmetz A. Nucleotide sequence of two anther-specific cDNAs from sunflower (Helianthus annuus L.). Plant Mol. Biol. 15:1990;643-646.
8. Epple P., Apel K., Bohlmann H. ESTs reveal a multigene family for plant defensins in Arabidopsis thaliana. FEBS Lett. 400:1997;168-172.
9. Epple P., Apel K., Bohlmann H. Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum. Plant Cell. 9:1997;509-520.
10. Florack D.E.A., Stiekema W.J. Thionins: properties, possible biological roles and mechanisms of action. Plant Mol. Biol. 26:1994;25-37.
11. Gu Q., Kawata E.E., Morse M.J., Wu H.M., Cheung A.Y. A flower-specific cDNA encoding a novel thionin in tobacco. Mol. Gen. Genet. 234:1992;89-96.
12. Harrison S.J., Marcus J.P., Goulter K.C., Green J.L., Maclean D.J., Manners J.M. An antimicrobial peptide from the Australian native Hardenbergia violacea provides the first functionally characterised member of a subfamily of plant defensins. Aust. J. Plant Physiol. 24:1997;571-578.
13. Karunanandaa B., Singh A., Kao T.H. Characterization of a predominantly pistil-expressed gene encoding a γ-thionin-like protein of Petunia inflata, Plant Mol. Biol. 26:1994;459-464.
14. Kombrink E., Somssich I.E. Defense responses of plants to pathogens. Andrews J.H, Tommerup I.C. Advances in Botanical Research (incorporating Advances in Plant Pathology). 1995;1-34 Academic Press, London. 21.
15. Lehrer R.I., Ganz T., Selsted M.E. Defensins: endogenous antibiotic peptides of animal cells. Cell. 64:1991;229-230.
16. Molina A., García-Olmedo F. Enhanced tolerance to bacterial pathogens caused by transgenic expression of barley lipid transfer protein LTP2. Plant J. 12:1997;669-675.
17. Molina A., Segura A., García-Olmedo F. Lipid transfer proteins (nsLTPs) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens. FEBS Lett. 316:1993;119-122.
18. Moreno M., Segura A., García-Olmedo F. Pseudothionin-St1, a potato peptide active against potato pathogens. Eur. J. Biochem. 223:1994;135-139.
19. Regente M.C., Oliva C.R., Feldman M.L., Castagnaro A.P., de la Canal L. A sunflower leaf antifungal peptide active against Sclerotinia sclerotiorum. Physiol. Plant. 100:1997;178-182.
20. Says-Lesage V., Tourvieille D. Recherche des sites de pollution et d'infection des fleurons de tournesol, in situ, par les spores de Sclerotinia sclerotiorum. CETIOM. 102:1988;4-13.
21. Segura A., Moreno M., Madueño F., Molina A., García-Olmedo F. Snakin-1, a peptide from potato that is active against plant pathogens. Mol. Plant Microbe Interact. 12:1999;16-23.
22. Segura A., Moreno M., Molina A., García-Olmedo F. Novel defensin subfamily from spinach (Spinacia oleracea). FEBS Lett. 435:1998;159-162.
23. Stiekema W.J., Heidekamp F., Dirkse W.G., Van Beckum J., De Haan P., Ten Bosch C., Louwerse J.D. Molecular cloning and analysis of four potato tuber mRNAs. Plant Mol. Biol. 11:1988;255-269.
24. Terras F.R.G., Eggermont K., Kovaleva V., Raikhel N.V., Osborn R.W., Kester A., Rees S.B., Torrekens S., Van Leuven F., Vanderleyden J., Cammue B.P.A., Broekaert W.F. Small cysteine-rich antifungal proteins from radish: their role in host defence. Plant Cell. 7:1995;573-588.
25. Terras F.R.G., Goderis I.J., Van Leuven F., Vanderleyden J., Cammue B.P.A., Broekaert W.F. In vitro anti-fungal activity of a radish (Raphanus sativus L.) seed protein homologous to nonspecific lipid transfer proteins. Plant Physiol. 100:1992;1055-1058.
26. Terras F.R.G., Schoofs H.M.E., De Bolle M.F.C., Van Leuven F., Rees S.B., Vanderleyden J., Cammue B.P.A., Broekaert W.F. Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. J. Biol. Chem. 267:1992;15301-15309.
27. Thomma B.P.H.J., Broekaert W.F. Tissue-specific expression of plant defensin genes PDF2.1 and PDF2.2 in Arabidopsis thaliana. Plant Physiol. Biochem. 36:1998;533-537.
28. von Heijne G. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 14:1986;4683-4690.