Analysis of inhibitory action of modified U1 snRNAs on target gene expression: Discrimination of two RNA targets differing by a 1 bp mismatch

Nucleic Acids Research

Volumn 30, Issue 11, 2002, Pages 2329-2339

  Liu, Peng ^a   Gucwa, Amy ^a   Stover, Mary Louise ^a   Buck, Emily ^a   Lichtler, Alexander ^a   Rowe, David ^a  

^a UNIVERSITY OF CONNECTICUT HEALTH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORAMPHENICOL ACETYLTRANSFERASE; GREEN FLUORESCENT PROTEIN; MESSENGER RNA; PROTEIN ANTIBODY; SMALL NUCLEAR RIBONUCLEOPROTEIN;

ANIMAL CELL; ARTICLE; BASE MISPAIRING; CONTROLLED STUDY; EXON; GENE CONSTRUCT; GENE EXPRESSION; GENE FUNCTION; GENE MUTATION; GENE REPRESSION; GENE TARGETING; GENE THERAPY; GENETIC TRANSDUCTION; HUMAN; INTRON; MOLECULAR HYBRIDIZATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; TRANSGENE;

3T3 CELLS; ANIMALS; BASE PAIR MISMATCH; BASE SEQUENCE; CHLORAMPHENICOL O-ACETYLTRANSFERASE; EXONS; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; GENES, REPORTER; HUMANS; INTRONS; MICE; MICROSCOPY, FLUORESCENCE; MUTATION; POLY A; RNA, MESSENGER; RNA, SMALL NUCLEAR; SUBSTRATE SPECIFICITY; TRANSFECTION; TRANSGENES;

ANIMALIA; FELIS CATUS;

EID: 0036606165     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/30.11.2329     Document Type: Article

References (44)

1. Reduction of target gene expression by a modified U1 snRNA
2. Sequences homologous to 5′ splice sites are required for the inhibitory activity of papillomavirus late 3′ untranslated regions
3. Poly(A) site selection in the HIV-1 provirus: Inhibition of promoter-proximal polyadenylation by the downstream major splice donor site
4. U1 snRNP inhibits pre-mRNA polyadenylation through a direct interaction between U1 70K and poly(A) polymerase
5. Stem-loop 1 of the U1 snRNP plays a critical role in the suppression of HIV-1 polyadenylation
6. Discrimination of heterogenous mRNAs encoding strychnine-sensitive glycine receptors in Xenopus oocytes by antisense oligonucleotides
7. Antisense oligodeoxynucleotides selectively suppress expression of the mutant alpha 2(I) collagen allele in type IV osteogenesis imperfecta fibroblasts. A molecular approach to therapeutics of dominant negative disorders
8. Discrimination of a single base change in a ribozyme using the gene for dihydrofolate reductase as a selective marker in Escherichia coli
9. Cleavage of collagen RNA transcripts by hammerhead ribozymes in vitro is mutation-specific and shows competitive binding effects
10. Design of hammerhead ribozymes to distinguish single base changes in substrate RNA
11. Hammerhead ribozymes selectively suppress mutant type I collagen mRNA in osteogenesis imperfecta fibroblasts
12. U1 small nuclear RNA genes are located on human chromosome 1 and are expressed in mouse-human hybrid cells
13. Functional elements of the human U1 RNA promoter. Identification of five separate regions required for efficient transcription and template competition
14. Multiple domains of U1 snRNA, including U1 specific protein binding sites, are required for splicing
15. An improved chloramphenicol acetyltransferase expression vector system for mapping transcriptional and post-transcriptional regulatory elements in animal cells
16. COL1A1 transgene expression in stably transfected osteoblastic cells. Relative contributions of first intron, 3′-flanking sequences and sequences derived from the body of the human COL1A1 minigene
17. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
18. Differential utilization of regulatory domains within the alpha 1(I) collagen promoter in osseous and fibroblastic cells
19. U1 small nuclear RNA chimeric ribozymes with substrate specificity for the Rev pre-mRNA of human immunodeficiency virus
20. In vivo targeting of SF/HGF and c-met expression via U1snRNA/ribozymes inhibits glioma growth and angiogenesis and promotes apoptosis
21. A hammerhead ribozyme suppresses expression of hepatocyte growth factor/scatter factor receptor c-MET and reduces migration and invasiveness of breast cancer cells
22. Inhibition of fibrillin 1 expression using U1 snRNA as a vehicle for the presentation of antisense targeting sequence
23. An element in the bovine papillomavirus late 3′ untranslated region reduces polyadenylated cytoplasmic RNA levels
24. The HIV-1 5′ LTR poly(A) site is inactivated by U1 snRNP interaction with the downstream major splice donor site
25. Position-dependent inhibition of the cleavage step of pre-mRNA 3′-end processing by U1 snRNP
26. Effects of nonsense mutations on nuclear and cytoplasmic adenine phosphoribosyltransferase RNA
27. Nonsense-mediated mRNA decay in health and disease
28. Nonsense mutations in the dihydrofolate reductase gene affect RNA processing
29. Effect of differential polyadenylation and cell growth phase on dihydrofolate reductase mRNA stability
30. Low hprt mRNA levels and multiple hprt mRNA species in 6-thioguanine-resistant Chinese hamster cell mutants possessing nonsense mutations
31. A catalogue of splice junction sequences
32. The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro
33. Construction of a novel database containing aberrant splicing mutations of mammalian genes
34. A single-base change at a splice site in a 0-thalassemic gene causes abnormal RNA splicing
35. A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation
36. Suppressor U1 snRNA in Drosophila
37. Suppression of mammalian 5′ splice-site defects by U1 small nuclear RNAs from a distance
38. Suppression of the 5′ splice site mutation in the Nagase analbuminemic rat with mutated U1 snRNA
39. Four new cases of lethal osteogenesis imperfecta due to glycine substitutions in COL1A1 and genes. Mutations in brief no. 152. Online
40. The molecular and genetic basis of fibroblast growth factor receptor 3 disorders: The achondroplasia family of skeletal dysplasias, Muenke craniosynostosis and Crouzon syndrome with acanthosis nigricans
41. A four base pair insertion polymorphism in the 3′ untranslated region of the COL1A1 gene is highly informative for null-allele testing in patients with osteogenesis imperfecta type I
42. A mutation-independent therapeutic strategem for osteogenesis imperfecta
43. Strategems in vitro for gene therapies directed to dominant mutations
44. A system for stable expression of short interfering RNAs in mammalian cells