Reduction of target gene expression by a modified U1 snRNA

Molecular and Cellular Biology

Volumn 21, Issue 8, 2001, Pages 2815-2825

  Beckley, S A ^a   Liu, P ^a   Stover, M L ^a   Gunderson, S I ^a   Lichtler, A C ^a   Rowe, D W ^a  

^a UNIVERSITY OF CONNECTICUT HEALTH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; CHLORAMPHENICOL ACETYLTRANSFERASE; GREEN FLUORESCENT PROTEIN; MESSENGER RNA; SMALL NUCLEAR RNA;

ANIMAL CELL; ARTICLE; BINDING SITE; CELL CLONE; CELL LINE; DNA CLEAVAGE; FIBROBLAST; GENE CONSTRUCT; GENE EXPRESSION; GENE INACTIVATION; GENE SEQUENCE; GENE TARGETING; GENETIC TRANSFECTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; REPORTER GENE; RIBONUCLEASE PROTECTION ASSAY; RNA TRANSCRIPTION; TRANSGENE;

NON-PROGRAMMATIC;

3T3 CELLS; ANIMALS; BASE SEQUENCE; BETA-GALACTOSIDASE; CHLORAMPHENICOL O-ACETYLTRANSFERASE; DNA; GENE EXPRESSION; GENE TARGETING; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MICE; RNA, SMALL NUCLEAR; TRANSFECTION;

ANIMALIA; FELIS CATUS;

EID: 0035083167     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.21.8.2815-2825.2001     Document Type: Article

References (81)

1. Stem-loop 1 of the U1 snRNP plays a critical role in the suppression of HIV-1 polyadenylation
2. Poly(A) site selection in the HIV-1 provirus: Inhibition of promoter-proximal polyadenylation by the downstream major splice donor site
3. The HIV-1 5′ LTR poly(A) site is inactivated by U1 snRNP interaction with the downstream major splice donor site
4. An improved chloramphenicol acetyltransferase expression vector system for mapping transcriptional and post-transcriptional regulatory elements in animal cells
5. The human immunodeficiency virus type 1 Rev protein and the Rev-responsive element counteract the effect of an inhibitory 5′ splice site in a 3′ untranslated region
6. Exon recognition in vertebrate splicing
7. Poly(A), poly(A) binding protein and the regulation of mRNA stability
8. The expression cassette determines the functional activity of ribozymes in mammalian cells by controlling their intracellular localization
9. Association of nuclear matrix antigens with exon-containing splicing complexes
10. The human U1 snRNP-specific U1A protein inhibits polyadenylation of its own pre-mRNA
11. Designing ribozymes for the inhibition of gene expression
12. A nuclear 3′-5′ exonuclease involved in mRNA degradation interacts with poly(A) polymerase and the hnRNA protein Np13p
13. A three-dimensional view of precursor messenger RNA metabolism within the mammalian nucleus
14. Regulation by HIV Rev depends upon recognition of splice sites
15. Performance and limits of the mixed-phase assay for chloramphenicol acetyltransferase at low [3H]acetyl-CoA concentration
16. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
17. U1 small nuclear RNAs with altered specificity can be stably expressed in mammalian cells and promote permanent changes in pre-mRNA splicing
18. Correction of the mutation responsible for sickle cell anemia by an RNA-DNA oligonucleotide
19. Utilization of splicing elements and polyadenylation signal elements in the coupling of polyadenylation and last-intron removal
20. Gene therapy for human immunodeficiency virus infection: Genetic antiviral strategies and targets for intervention
21. Analysis of mammalian cis-regulatory DNA elements by homologous recombination
22. Sequences homologous to 5′ splice sites are required for the inhibitory activity of papillomavirus late 3′ untranslated regions
23. Analysis of cytoplasmic and nuclear messenger RNA in fibroblasts from patients with type I osteogenesis imperfecta
24. The effects of 5′-capping, 3′-polyadenylation and leader composition upon the translation and stability of mRNA in a cell-free extract derived from the yeast Saccharomyces cerevisiae
25. Single base discrimination for ribonuclease H-dependent antisense effects within intact human leukaemia cells
26. The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection
27. In vivo selection of RNAs that localize in the nucleus
28. The human U1A snRNP protein regulates polyadenylation via a direct interaction with poly(A) polymerase
29. U1 snRNP inhibits pre-mRNA polyadenylation through a direct interaction between U1 70K and poly(A) polymerase
30. Involvement of the carboxyl terminus of vertebrate poly(A) polymerase in U1A autoregulation and in the coupling of splicing and polyadenylation
31. Multiple domains of U1 snRNA, including U1 specific protein binding sites, are required for splicing
32. An E. coli beta-galactosidase cassette suitable for study of eukaryotic expression
33. Suppression of the 5' splice site mutation in the Nagase analbuminemic rat with mutated U1snRNA
34. Mechanisms for selecting 5′ splice sites in mammalian pre-mRNA splicing
35. U1 and U2 small nuclear RNAs are present in nuclear speckles
36. Role of polyadenylation in nucleocytoplasmic transport of mRNA
37. A suboptimal 5′ splice site is a cis-acting determinant of nuclear export of polyomavirus fate mRNAs
38. Tracking COL1A1 RNA in osteogenesis imperfecta: Splice-defective transcripts initiate transport from the gene but are retained within the SC35 domain
39. Retrovirally expressed anti-HIV ribozymes confer a selective survival advantage on CD4+ T cells in vitro
40. Fourteen residues of the U1 snRNP-specific U1A protein are required for homodimerization, cooperative RNA binding, and inhibition of polyadenylation
41. Disruption of base pairing between the 5′ splice site and the 5′ end of U1 snRNA is required for spliceosome assembly
42. Factors governing the activity in vivo of ribozymes transcribed by RNA polymerase III
43. Gene repair using chimeric RNA/DNA oligonucleotides
44. Antisense RNA: Function and fate of duplex RNA in cells of higher eukaryotes
45. Efficient site-directed mutagenesis using uracil-containing DNA
46. Formation of a catalytically active dimer by tRNA(Val)-driven short ribozymes
47. Specific inhibition of expression of a human collagen gene (COL1A1) with modified antisense oligonucleotides. The most effective target sites are clustered in double-stranded regions of the predicted secondary structure for the mRNA
48. Hierarchy for 5′ splice site preference determined in vivo
49. mRNA localization signals can enhance the intracellular effectiveness of hammerhead ribozymes
50. Simple, inexpensive preparation of T1/T2 ribonuclease suitable for use in RNase protection experiments
51. Extending the cleavage rules for the hammerhead ribozyme: Mutating adenosine 15.1 to inosine 15.1 changes the cleavage site specificity from N16.2U16.1H17 to N16.2C16.1H17
52. Heterogeneity of human U1 snRNAs
53. True genes for human U1 small nuclear RNA. Copy number, polymorphism, and methylation
54. Direct interaction of the U1 snRNP-A protein with the upstream efficiency element of the SV40 late polyadenylation signal
55. Interaction between the U1 snRNP-A protein and the 160-kD subunit of cleavage-polyadenylation specificity factor increases polyadenylation efficiency in vitro
56. Nucleoplasmic organization of small nuclear ribonucleoproteins in cultured human cells
57. Design, characterization and testing of tRNA3Lys-based hammerhead ribozymes
58. Inhibition of human immunodeficiency virus type 1 replication by nuclear chimeric anti-HIV ribozymes in a human T lymphoblastoid cell line
59. U1 small nuclear RNA chimeric ribozymes with substrate specificity for the Rev pre-mRNA of human immunodeficiency virus
60. Inhibition of fibrillin 1 expression using U1 snRNA as a vehicle for the presentation of antisense targeting sequence
61. The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro
62. Ten years of gene targeting: Targeted mouse mutants, from vector design to phenotype analysis
63. Transcription of a gene for human U1 small nuclear RNA
64. Synergistic stimulation of HIV-1 rev-dependent export of unspliced mRNA to the cytoplasm by hnRNP A1
65. Bacterial beta-galactosidase as a marker of Rous sarcoma virus gene expression and replication
66. Identification of a novel, non-snRNP protein complex containing U1A protein
67. Control of the functional activity of an antisense RNA by a tetracycline-responsive derivative of the human U6 snRNA promoter
68. Differential effects of polyadenylation regions on gene expression in mammalian cells
69. An improved fluor diffusion assay for chloramphenicol acetyltransferase gene expression
70. Nuclear retention of COL1A1 messenger RNA identifies null alleles causing mild osteogenesis imperfecta
71. Exon definition may facilitate splice site selection in RNAs with multiple exons
72. In vitro selection of an allosteric ribozyme that transduces analytes to amplicons
73. Who's on first? The U1 snRNP-5′ splice site interaction and splicing
74. Involvement of U1 small nuclear ribonucleoproteins (snRNP) in 5′ splice site-U1 snRNP interaction
75. Introduction of DNA into mammalian cells
76. Processing of endogenous pre-mRNAs in association with SC-35 domains is gene specific
77. Two problems in antisense biotechnology: In vitro delivery and the design of antisense experiments
78. Defective splicing of mRNA from one COL1A1 allele of type I collagen in nondeforming (type I) osteogenesis imperfecta
79. Double-target antisense U7 snRNAs promote efficient skipping of an aberrant exon in three human beta-thalassemic mutations
80. Importance of exocyclic base functional groups of central core guanosines for hammerhead ribozyme activity