Design of hammerhead ribozymes to distinguish single base changes in substrate RNA

Antisense and Nucleic Acid Drug Development

Volumn 6, Issue 3, 1996, Pages 165-168

  Singh, Kumud K ^a,b   Schluff, Petra ^a   Lehnert, Lasse ^a   Krupp, Guido ^a  

^a UNIVERSITY OF KIEL   (Germany)

^b UNIVERSITY OF DELHI   (India)

Author keywords

[No Author keywords available]

Indexed keywords

RIBOZYME; RNA;

ARTICLE; BASE PAIRING; CONTROLLED STUDY; GENE EXPRESSION REGULATION; GENE SEQUENCE; NUCLEIC ACID BASE SUBSTITUTION; PRIORITY JOURNAL; RNA CLEAVAGE;

EID: 0029810775     PISSN: 10872906     EISSN: None     Source Type: Journal    
DOI: 10.1089/oli.1.1996.6.165     Document Type: Article

References (6)

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2. BUZAYAN, J.M., GERLACH, W.L., and BRUENING, G. (1986). Non-enzymatic cleavage and ligation of RNAs complementary to a plant virus satellite RNA. Nature 323, 349-353.
3. FUNATO, T., SHITARA, T., TONE, T., JIAO, L., KASHANISABET, M., and SCANLON, K.J. (1994). Suppression of H-ras-mediated transformation in NIH3T3 cells by a ras ribozyme. Biochem. Pharmacol. 48, 1471-1475.
4. HARTMANN, R.K., KRUPP, G., and HARDT, W.-D. (1995). Towards a new concept of gene inactivation: Specific cleavage by endogenous ribonuclease P. Biotech. Annu. Rev. 1,215-265.
5. HASELOFF, J., and GERLACH, W. (1988). Simple RNA enzymes with new and highly specific endoribonuclease activities. Nature 334, 585-591.
6. ZOUMADAKIS, M., and TABLER, M. (1995). Comparative analysis of cleavage rates after systematic permutation of the NUX consensus target motif for hammerhead ribozymes. Nucleic Acids Res. 23, 1192-1196.