CENP-C binds the alpha-satellite DNA in vivo at specific centromere domains

Journal of Cell Science

Volumn 115, Issue 11, 2002, Pages 2317-2327

  Politi, Valeria ^a   Perini, Giovanni ^a   Trazzi, Stefania ^a   Pliss, Artem ^a   Raska, Ivan ^a   Earnshaw, William C ^a   Della Valle, Giuliano ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Alpha satellite DNA; CENP B; CENP C; Centromere; Chromatin immunoprecipitation

Indexed keywords

AMINO ACID; CHROMOSOME PROTEIN; DNA; SATELLITE DNA; AUTOANTIGEN; CENPB PROTEIN, HUMAN; CENTROMERE PROTEIN B; CENTROMERE PROTEIN C; DNA BINDING PROTEIN; NONHISTONE PROTEIN;

ARTICLE; BIOCHEMISTRY; CELL ULTRASTRUCTURE; CENTROMERE; CHROMOSOME SATELLITE ASSOCIATION; COMPLEX FORMATION; CONTROLLED STUDY; DNA BINDING; DNA MICROARRAY; DNA PROTEIN COMPLEX; DNA SEQUENCE; DNA STRUCTURE; HELA CELL; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; IN VIVO STUDY; INTERPHASE; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; BIOASSAY; CELL DIVISION; CYTOLOGY; EUKARYOTIC CELL; GENETICS; METABOLISM; NUCLEOTIDE SEQUENCE; PROTEIN TERTIARY STRUCTURE;

BASE SEQUENCE; BIOLOGICAL ASSAY; CELL DIVISION; CENTROMERE; CHROMOSOMAL PROTEINS, NON-HISTONE; DNA, SATELLITE; DNA-BINDING PROTEINS; EUKARYOTIC CELLS; HELA CELLS; HUMAN; PROTEIN STRUCTURE, TERTIARY; SUPPORT, NON-U.S. GOV'T; AUTOANTIGENS; CENTROMERE PROTEIN B; HUMANS;

EID: 0036591963     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (72)

1. Chromosomal location by in situ hybridization of the human Sau3 family of DNA repeats
2. Immunocytochemical analysis of the coiled body in the cell cycle and during cell proliferation
3. Comparative mapping of human alphoid sequences in Great Apes using fluorescence in situ hybridization
4. Regulation of hormone-induced hystone hyperacetylation and gene activation via acetylation of an acetylase
5. Centromere DNA dynamics: Latent centromeres and neocentromere formation
6. Centromerization
7. Regional mapping of the human renin gene to 1q32 by in situ hybridization
8. CENP-B: A major human centromere protein located beneath the kinetochore
9. Mapping DNA within the mammalian kinetochore
10. A simplified formaldehyde-fixation and immunoprecipitation technique for studying protein-DNA interactions
11. Characterization of neo-centromeres in marker chromosomes lacking detectable alpha-satellite DNA
12. Poly(ADP-ribose) polymerase at active centromeres and neocentromeres at metaphase
13. Three related centromere proteins are absent from the inactive centromere of a stable isodicentric chromosome
14. Visualization of centromere proteins CENP-B and CENP-C on a stable dicentric chromosome in cytological spreads
15. Centromeric inactivation in a dicentric human Y:21 translocation chromosome
16. Efficient conditional mutation of the vertebrate CENP-C gene
17. CENP-C is necessary but not sufficient to induce formation of a functional centromere
18. CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells
19. Formation of de novo centromeres and construction of first-generation human artificial microchromosomes
20. Molecular analysis of kinetochore-microtubule attachment in budding yeast
21. Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice
22. Ku is associated with the telomere in mammals
23. Construction of YAC-based mammalian artificial chromosomes
24. Ubiquitous, interspersed repeated sequences in mammalian genomes
25. Targeted disruption of mouse centromere protein C gene leads to mitotic disarray and early embryo death
26. The case for epigenetic effects on centromere identity and function
27. A 330 kb CENP-A binding domain and altered replication timing at a human neocentromere
28. Assay of centromere function using a human artificial chromosome
29. HCP-4, a CENP-C-like protein in Caenorhabditis elegans, is required for resolution of sister centromeres
30. Centromere protein B assembles human centromeric a satellite DNA at the 17-bp sequence, CENP-B box
31. A functional assay of kinetochore assembly in Caenorhabditis elegans
32. Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation
33. Analysis of chromatin structure by in vivo formaldehyde cross-linking
34. Chromosome stability is maintained by short intercentromeric distance in functionally dicentric human Robertsonian translocations
35. Further evidence that CENP-C is a necessary component of active centromeres: Studies of a dic(X;15) with simultaneous immunofluorescence and FISH
36. Virus infection leads to localized hyperacetylation of histones H3 and H4 at the IFN-β promoter
37. Distinct protein interaction domains and protein spreading in a complex centromere
38. Structure of the human centromere at metaphase
39. Identification of a subdomain of CENP-B that is necessary and sufficient for localization to the human centromere
40. The formation, structure and composition of the mammalian kinetochore fiber
41. In vivo association of lamins with nucleic acids in Drosophila melanogaster
42. CENP-C, an autoantigen in scleroderma, is a component of the human inner kinetochore plate
43. ScII: An abundant chromosome scaffold protein is a member of a family of putative ATPases with an unusual predicted tertiary structure
44. Mapping protein-DNA interactions in vivo with formaldehyde: Evidence that histone H4 is retained on a highly transcribed gene
45. SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast
46. The Polycomb group protein complex of Drosophila melanogaster has different compositions at different target genes
47. Human centromere protein C (CENP-C) is a DNA-binding protein, which possesses a novel DNA-binding motif
48. Characterization of internal DNA-binding and C-terminal dimerization domains of human centromere/kinetochore autoantigen CENP-C in vitro: Role of DNA-binding and self-associating activities in kinetochore organization
49. Visualization of pre-kinetochore locus on the centromeric region of highly extended chromatin fibers: Does kinetochore autoantigen CENP-C constitute a kinetochore organizing center?
50. A solid fundation: Functional specialization of centromeric chromatin
51. Identification of centromeric antigens in dicentric Robertsonian translocations: CENP-C and CENP-E are necessary components of functional centromeres
52. Stable dicentric X chromosomes with two functional centromeres
53. Determining centromere identity: Cyclical stories and forking path
54. Identification of cohesin association sites at centromeres and along chromosome arms
55. Use of poly(vinylpyrrolidone) and poly(vinyl alcohol) for cryoultramicrotomy
56. CENP-C is required for maintaining proper kinetochore size and for a timely transition to anaphase
57. The specificity of protein-DNA crosslinking by formaldehyde: In vitro and in drosophila embryos
58. Chromatin containing CENP-A and alpha-satellite DNA is a major component of the inner kinetochore plate
59. INCENP loss from an inactive centromere correlates with the loss of sister chromatid cohesion
60. Specification of kinetochore-forming chromatin by the histone H3 variant CENP-A
61. Immunolocalization of CENP-A suggests a distinct nucleosome structure at the inner kinetochore plate of active centromeres
62. Genomic organization of α-satellite DNA on human chromosome 7: Evidence for two distinct alphoid domains on a single chromosome
63. Physical map of the centromeric region of human chromosome 7: Relationship between two distinct alpha-satellite arrays
64. Structure of DNA near long tandem arrays of a satellite DNA at the centromeres of human chromosome 7
65. Centromeric chromatin and epigenetic effects kinetochore assembly
66. Chromosome-specific organization of human a satellite DNA
67. Hierarchical order in chromosome-specific human a satellite DNA
68. Sequence definition and organization of a human repeated DNA
69. Identification of overlapping DNA-binding and centromere-targeting domains in the human kinetochore protein CENP-C
70. A human centromere protein, CENP-B, has a DNA binding domain containing four potential alpha helices at the NH2 terminus, which is separable from dimerizing activity