Characterization of internal DNA-binding and C-terminal dimerization domains of human centromere/kinetochore autoantigen CEN P-C in vitro: Role of DNA-binding and self-associating activities in kinetochore organization

Chromosome Research

Volumn 5, Issue 2, 1997, Pages 132-141

  Sugimoto, Kenji ^a   Kuriyama, Kenji ^a   Shibata, Akiko ^a   Himeno, Michio ^a  

^a Osaka Prefecture University   (Japan)

Author keywords

Autoantigen; Centromere; Cross linking; Dimerization; Kinetochore

Indexed keywords

AUTOANTIGEN; PROTEIN C;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CENTROMERE; DIMERIZATION; DNA BINDING; DNA CROSS LINKING; GEL FILTRATION; IMMUNOBLOTTING; PRIORITY JOURNAL;

EID: 0030935916     PISSN: 09673849     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1018422325569     Document Type: Article

References (25)

1. Brenner S, Pepper D, Berns MW, Tan E, Brinkley BR (1981) Kinetochore structure, duplication, and distribution in mammalian cells: analysis by human autoantibodies from scleroderma patients. J Cell Biol 91: 95-102.
2. Brown MT (1995) Sequence similarities between the yeast chromosome segregation protein Mif2 and the mammalian centromere protein CENP-C. Gene 160: 111-116.
3. Cooke CA, Bernat L, Earnshaw WC (1990) CENP-B: a major human centromere protein located beneath the kinetochore. J Cell Biol 110: 1475-1488.
4. Cooke CA, Bazett-Jones DP, Earnshaw WC, Rattner JB (1993) Mapping DNA within the mammalian kinetochore. J Cell Biol 120: 1083-1091.
5. Cornell R (1989) Chemical cross-linking reveals a dimeric structure for CTP: phosphocholine cytidyltransferase. J Biol Chem 264: 9077-9082.
6. Earnshaw WC, Rothfield N (1985) Identification of a family of human centromere proteins using autoimmune sera from patients with scleroderma. Chromosoma 91: 313-321.
7. Kim S, Willison KR, Horwich AL (1994) Cytosolic chaperonin subunits have a conserved ATPase domain but diverged polypeptide-binding domains. Trends Biol Sci 19: 543-547.
8. Kitagawa K, Masumoto H, Ikeda M, Okazaki T (1995) Analysis of protein-DNA and protein-protein interactions of centromere protein B (CENP-B) and properties of the DNA-CENP-B complex in the cell cycle. Mol Cell Biol 15: 1602-1612.
9. Lanini L, McKeon F.(1995) Domains required for CENP-C assembly at the kinetochore. Mol Biol Cell 6: 1049-1059.
10. McKay S, Thomson E, Cooke H (1994) Sequence homologies and linkage group conservation of the human and mouse cenpc genes. Genomics 22: 36-40.
11. Masumoto H, Masukata H, Muro Y, Nozaki N, Okazaki T (1989) A human centromere antigen (CENP-B) interacts with a short specific sequence in alphoid DNA, a human centromeric satellite. J Cell Biol 109: 1963-1973.
12. Moroi Y, Peebles C, Fritzler MJ, Steigerwald J, Tan EM (1980) Autoantibody to centromere (kinetochore) in scleroderma sera. Proc Natl Acad Sci USA 77: 1627-1631.
13. Muro Y, Sugimoto K, Okazaki T, Ohashi M (1990) The heterogeneity of anticentromere antibodies in immunoblotting analysis. J Rheumatol 17: 1042-1047.
14. Page SL, Earnshaw WC, Choo KHA, Shaffer LG (1995) Further evidence that CENP-C is a necessary component of active centromeres: studies of a dic(X;15) with simultaneous immunofluorescence and FISH. Hum Mol Genet 4: 289-294.
15. Pilch P, Czech MP (1980) The subunit structure of the high affinity insulin receptor. J Biol Chem 255: 1722-1731.
16. Rieder CL (1982) The formation, structure, and composition of the mammalian kinetochore and kinetochore fiber. Int Rev Cytol 79: 1-58.
17. Saitoh H, Tomkiel J, Cooke CA et al. (1992) CENP-C, an autoantigen in scleroderma, is a component of the human inner kinetochore plate. Cell 70: 115-125.
18. Sugimoto K (1994) DNA immunoprecipitation: application to characterization of target sequences for a human centromere DNA-binding protein (CENP-B). In: Celis JE, ed. Cell Biology: A Laboratory Handbook. San Diego: Academic Press, pp 335-343.
19. Sugimoto K, Muro Y, Himeno M (1992) Anti-helix-loop-helix domain antibodies: discovery of autoantibodies that inhibit DNA binding activity of human centromere protein B (CENP-B). J Biochem 111, 478-483.
20. Sugimoto K, Hagishita Y, Himeno M (1994a) Functional domain structure of human centromere protein B: implication of the internal and C-terminal self-association domains in centromeric heterochromatin condensation. J Biol Chem 269: 24271-24276.
21. Sugimoto K, Yata H, Muro Y, Himeno M (1994b) Human centromere protein C (CENP-C) is a DNA-binding protein which possesses a novel DNA-binding motif. J Biochem 116: 877-881.
22. Sugimoto K, Yamada T, Muro Y, Himeno M (1996) Human homolog of Drosophila heterochromatin-associated protein 1 (HP1) is a DNA-binding protein which possesses a DNA-binding motif with weak similarity to that of human centromere protein C (CENP-C). J Biochem 120: 153-159.
23. Tomkiel J, Cooke CA, Saitoh H, Bernat RL, Earnshaw WC (1994) CENP-C is required for maintaining proper kinetochore size and for a timely transition to anaphase. J Cell Biol 125: 531-545.
24. Verheijen R, de Jong BAW, Oberye EHH, van Venrooij WJ (1992) Molecular cloning of a major CENP-B epitope and its use for the detection of anticentromere autoantibodies. Mol Biol Rep 16: 49-59.
25. Yang CH, Tomkiel J, Saitoh H, Johnson DH, Earnshaw WC (1996) Identification of overlapping DNA-binding and centromere-targeting domains in the human kinetochore protein CENP-C. Mol Cell Biol 16: 3576-3586.