Ralstonia solanacearum requires type 4 pili to adhere to multiple surfaces and for natural transformation and virulence

Molecular Microbiology

Volumn 46, Issue 2, 2002, Pages 427-437

  Kang, Yaowei ^a,d   Liu, Huanli ^a   Genin, Stéphane ^b   Schell, Mark A ^a,c   Denny, Timothy P ^a  

^a UNIVERSITY OF GEORGIA   (United States)

^b CNRS   (France)

^c UNIVERSITY OF GEORGIA   (United States)

^d IOWA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; PILIN; PROTEIN PILA; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ADHERENCE; BACTERIUM COLONY; BACTERIUM PILUS; BACTERIUM TRANSFORMATION; BIOFILM; CELL DENSITY; CONTROLLED STUDY; ELECTRON MICROSCOPY; NONHUMAN; OPEN READING FRAME; PLANT ROOT; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN EXPRESSION; PROTEIN MOTIF; PSEUDOMONAS AERUGINOSA; RALSTONIA SOLANACEARUM; TOBACCO; TOMATO; TRANSCRIPTION REGULATION;

AMINO ACID SEQUENCE; BACTERIAL ADHESION; BACTERIAL PROTEINS; BASE SEQUENCE; BETAPROTEOBACTERIA; DNA-BINDING PROTEINS; FIMBRIAE PROTEINS; FIMBRIAE, BACTERIAL; GENE EXPRESSION REGULATION, BACTERIAL; LYCOPERSICON ESCULENTUM; MICROSCOPY, ELECTRON; MOLECULAR SEQUENCE DATA; PLANT DISEASES; SIGNAL TRANSDUCTION; TOBACCO; TRANSFORMATION, BACTERIAL; VIRULENCE;

BACTERIA (MICROORGANISMS); LYCOPERSICON ESCULENTUM; NEGIBACTERIA; NICOTIANA TABACUM; PILA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; RALSTONIA; RALSTONIA SOLANACEARUM;

EID: 0036429982     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2002.03187.x     Document Type: Article

References (51)

1. Aldon, D., Brito, B., Boucher, C., and Genin, S. (2000) A bacterial sensor of plant cell contact controls the transcriptional induction of Ralstonia solanacearum pathogenicity genes. EMBO J 19:2304-2314.
2. Allen, C., Gay, J., and Simon-Buela, L. (1997) A regulatory locus, pehSR, controls polygalacturonase production and other virulence functions in Ralstonia solanacearum. Mol Plant-Microbe Interact 10:1054-1064.
3. Alm, R.A., and Mattick, J.S. (1997) Genes involved in the biogenesis and function of type 4 fimbriae in Pseudomonas aeruginosa. Gene 192:89-98.
4. Ausubel, F.M., Brent, R., Kingston, R.E., More, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (1989) Short Protocols in Molecular Biology. New York: Green Publishing Association and Wiley Interscience.
5. 54-dependent promoter sequences. Nucleic Acids Res 27:4305-4313.
6. Bertolla, F., van Gijsegem, F., Nesme, X., and Simonet, P. (1997) Conditions for natural transformation of Ralstonia solanacearum. Appl Environ Microbiol 63:4965-4968.
7. Bieber, D., Ramer, S.W., Wu, C.Y., Murray, W.J., Tobe, T., Fernandez, R., and Schoolnik, G.K. (1998) Type IV pili, transient bacterial aggregates, and virulence of entero-pathogenic Escherichia coli. Science 280:2114-2118.
8. Blair, D.F. (1995) How bacteria sense and swim. Annu Rev Microbiol 49:489-522.
9. Brumbley, S.M., and Denny, T.P. (1990) Cloning of phcA from wild-type Pseudomonas solanacearum, a gene that when mutated alters expression of multiple traits that contribute to virulence. J Bacteriol 172:5677-5685.
10. Chen, W.P., and Kuo, T.T. (1993) A simple and rapid method for the preparation of gram-negative bacterial genomic DNA. Nucleic Acids Res 21:2260.
11. Clough, S.J., Schell, M.A., and Denny, T.P. (1994) Evidence for involvement of a volatile extracellular factor in Pseudomonas solanacearum virulence gene expression. Mol Plant-Microbe Interact 7:621-630.
12. Clough S.J., Flavier, A.B., Schell, M.A., and Denny, T.P (1997a) Differential expression of virulence genes and motility in Ralstonia (Pseudomonas) solanacearum during exponential growth. Appl Environ Microbiol 63:844-850.
13. Clough, S.J., Lee, K.-E., Schell, M.A., and Denny, T.P. (1997b) A two-component system in Ralstonia (Pseudomonas) solanacearum modulates production of PhcA-regulated virulence factors in response to 3-hydroxypalmitic acid methyl ester. J Bacteriol 179:3639-3648.
14. Cornelis, G.R., and van Gijsegem, F. (2000) Assembly and function of type III secretory systems. Annu Rev Microbiol 54:735-774.
15. Fernandez, L.A., and Berenguer, J. (2000) Secretion and assembly of regular surface structures in Gram-negative bacteria. FEMS Microbiol Rev 24:21-44.
16. Finlay, B., and Caparon, M. (2000) Bacterial adherence to cell surfaces and extracellular matrix. In Cellular Microbiology. Cossart, P., Boquet, P., Normark, S., and Rappuoli, R. (eds). Washington, DC: American Society for Microbiology Press, pp. 67-80.
17. Flavier, A.B., Clough, S.J., Schell, M.A., and Denny, T.P. (1997) Identification of 3-hydroxypalmitic acid methyl ester as a novel autoregulator controlling virulence in Ralstonia solanacearum. Mol Microbiol 26:251-259.
18. Fussenegger, M., Rudel, T., Barten, R., Ryll, R., and Meyer, T.F. (1997) Transformation competence and type 4 pilus biogenesis in Neisseria gonorrhoeae - A review. Gene 192:125-134.
19. van Gijsegem, F., Gough, C., Zischek, C., Niqueuz, E., Arlat, M., Genin, S., et al. (1995) The hrp gene locus of Pseudomonas solanacearum, which controls the production of a type III secretion system, encodes eight proteins related to components of the bacterial flagellar biogenesis complex. Mol Microbiol 15:1095-1114.
20. van Gijsegem, F., Vasse, J., Camus, J.C., Marenda, M., and Boucher, C. (2000)Ralstonia solanacearum produces Hrp-dependent pili that are required for PopA secretion but not for attachment of bacteria to plant cells. Mol Microbiol 36: 249-260.
21. Glessner, A., Smith, R.S., Iglewski, B.H., and Robinson, J.B. (1999) Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of twitching motility. J Bacteriol 181:1623-1629.
22. Graupner, S., and Wackernagel, W. (2001) Pseudomonas stutzeri has two closely related pilA genes (type IV pilus structural protein) with opposite influences on natural genetic transformation. J Bacteriol 183:2359-2366.
23. Hahn, H.P. (1997) The type-4 pilus is the major virulence-associated adhesin of Pseudomonas aeruginosa - A review. Gene 192:99-108.
24. Henderson, B., Wilson, M., McNab, R., and Lax, A.J. (1999) Cellular Microbiology. Bacteria-Host Interactions in Health and Disease. New York: John Wiley & Sons.
25. Jin, S., Ishimoto, K.S., and Lory, S. (1994) PilR, a transcriptional regulator of piliation in Pseudomonas aeruginosa, binds to a cis-acting sequence upstream of the pilin gene promoter. Mol Microbiol 14:1049-1057.
26. Kachlany, S.C., Planet, P.J., DeSalle, R., Fine, D.H., and Figurski, D.H. (2001a) Genes for tight adherence of Acti-nobacillus actinomycetemcomitans: From plaque to plague to pond scum. Trends Microbiol 9:429-437.
27. Kachlany, S.C., Planet, P.J., DeSalle, R., Fine, D.H., Figurski, D.H., and Kaplan, J.B. (2001b) flp-1, the first representative of a new pilin gene subfamily, is required for non-specific adherence of Actinobacillus actinomycetemcomitans. Mol Microbiol 40:542-554.
28. Kalogeraki, V.S., and Winans, S.C. (1997) Suicide plasmids containing promoterless reporter genes can simultaneously disrupt and create fusions to target genes of diverse bacteria. Gene 188:69-75.
29. Keen, N.T., Tamaki, S., Kobayashi, D., and Trollinger, D. (1988) Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene 70:191-197.
30. Kitten, T., and Willis, D.K. (1996) Suppression of a sensor kinase-dependent phenotype in Pseudomonas syringae by ribosomal proteins L35 and L20. J Bacteriol 178: 1548-1555.
31. Liu, H., Kang, Y., Genin, S., Schell, M.A., and Denny, T.P. (2001) Twitching motility of Ralstonia solanacearum requires a type IV pilus system. Microbiology 147:3215-3229.
32. Low, D., Braaten, B., and van der Woude, M. (1996) Fimbriae. In Escherichia coli and Salmonella: Cellular and Molecular Biology. Neidhardt, F.C., Curtis, R.I., Ingram, J.L., Lin, E.C.C., Low, K.B., Magasanik, B., et al. (eds). Washington, DC: American Society for Microbiology Press, pp. 146-157.
33. McGarvey, J.A., Denny, T.P., and Schell, M.A. (1999) Spatial-temporal and quantitative analysis of growth and EPS I production by Ralstonia solanacearum, resistant and susceptible tomato cultivars. Phytopathology 89: 1233-1239.
34. Manning, P.A., and Meyer, T.F. (1997) Type 4 pili: Biogenesis, adhesins, protein export and DNA import. Proceedings of a workshop. Gene 192:1-198.
35. Merz, A.J., So, M., and Sheetz, M.P. (2000) Pilus retraction powers bacterial twitching motility. Nature 407:98-102.
36. Meyer, T.F., Billyard, E., Haas, R., Storzbach, S., and So, M. (1984) Pilus genes of Neisseria gonorrhoeae: Chromosomal organization and DNA sequence. Proc Natl Acad Sci USA 81:6110-6114.
37. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
38. Nagata, T., Nemoto, Y., and Hasezawa, S. (1992) Tobacco BY-2 cell-line as the 'HeLa'-cell in the cell biology of higher-plants. Int Rev Cytol 132:1-30.
39. O'Toole, G.A., and Kolter, R. (1998) Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Mol Microbiol 30:295-304.
40. Ojanen-Reuhs, T., Kalkkinen, N., Westerlund-Wikstrom, B., van Doorn, J., Haahtela, K., Nurmiaholassila, E.L., et al. (1997) Characterization of the fimA gene encoding bundle-forming fimbriae of the plant pathogen Xanthomonas campestris pv. vesicatoria. J Bacteriol 179:1280-1290.
41. Ramakrishnan, G., Zhao, J.L., and Newton, A. (1994) Multiple structural proteins are required for both transcriptional activation and negative autoregulation of Caulobacter crescentus flagellar genes. J Bacteriol 176:7587-7600.
42. Ritchings, B.W., Almira, E.C., Lory, S., and Ramphal, R. (1995) Cloning and phenotypic characterization of fleS and fleR, new response regulators of Pseudomonas aerugi-nosa which regulate motility and adhesion to mucin. Infect Immun 63:4868-4876.
43. Schell, M.A. (2000) Control of virulence and pathogenicity genes of Ralstonia solanacearum by an elaborate sensory array. Annu Rev Phytopathol 38:263-292.
44. Snedecore, G.W., and Cochran, W.G. (1980) Statistical Methods, 7th edn. Ames, IA: The Iowa State University Press.
45. Spangenberg, C., Fislage, R., Sierralta, W., Tümmler, B., and Römling, U. (1995) Comparison of type IV pilin genes of Pseudomonas aeruginosa of various habitats has uncovered a novel unusual sequence. FEMS Microbiol Lett 125: 265-274.
46. Strom, M.S., and Lory, S. (1993) Structure-function and biogenesis of the type IV pili. Annu Rev Microbiol 47: 565-596.
47. Tonjum, T., Weir, S., Bovre, K., Progulske-Fox, A., and Marrs, C.F. (1993) Sequence divergence in two tandemly located pilin genes of Eikenella corrodens. Infect Immun 61:1909-1916.
48. Wall, D., and Kaiser, D. (1999) Type IV pili and cell motility. Mol Microbiol 32:1-10.
49. Weir, S., Lee, L.W., and Marrs, C.F. (1996) Identification of four complete type 4 pilin genes in a single Kingella denitrificans genome. Infect Immun 64:4993-4999.
50. Whitchurch, C.B., Alm, R.A., and Mattick, J.S. (1996) The alginate regulator AlgR and an associated sensor FimS are required for twitching motility in Pseudomonas aeruginosa. Proc Natl Acad Sci USA 93:9839-9843.
51. Wolfgang, M., van Putten, J.P.M., Hayes, S.F., and Koomey, M. (1999) The comP locus of Neisseria gonorrhoeae encodes a type IV prepilin that is dispensable for pilus biogenesis but essential for natural transformation. Mol Microbiol 31:1345-1357.