Pilus retraction powers bacterial twitching motility

Nature

Volumn 407, Issue 6800, 2000, Pages 98-102

  Merz, Alexey J ^a,c   So, Magdalene ^a   Sheetz, Michael P ^b,d  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^b DUKE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c DARTMOUTH MEDICAL SCHOOL   (United States)

^d Columbia University ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; PILT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM ADHERENCE; BACTERIUM PILUS; GRAM NEGATIVE BACTERIUM; NEISSERIA GONORRHOEAE; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS;

FIMBRIAE, BACTERIAL; MOVEMENT; NEISSERIA GONORRHOEAE;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; NEISSERIA GONORRHOEAE;

EID: 0034618643     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/35024105     Document Type: Article

References (30)

1. Wall, D. & Kaiser, D. Type IV pili and cell motility. Mol. Microbiol. 32, 1-10 (1999).
2. Henrichsen, J. Twitching motility. Annu. Rev. Microbiol. 37, 81-93 (1983).
3. Bradley, D. E. A function of Pseudomonas aeruginosa PAO polar pili: twitching motility. Can. J. Microbiol. 26, 146-154 (1980).
4. Wolfgang, M., Park, H. S., Hayes, S. E., van Putten, J. P. M. & Koomey, M. Suppression of an absolute defect in type IV pilus biogenesis by loss-of-function mutations in pilT, a twitching motility gene in Neisseria gonorrhoeae. Proc. Natl Acad. Sci. USA 95, 14973-14978 (1998).
5. Sheetz, M. P. (ed.) Laser Tweezers in Cell Biology (Academic, New York, 1997).
6. Swanson, J. Studies on gonococcus infection. XII. Colony color and opacity variants of gonococci. Infect. Immun. 19, 320-331 (1978).
7. O'Toole, G. A. & Kolter, R. Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Mol. Microbiol. 30, 295-304 (1998).
8. Bieber, D. et al. Type IV pili, transient bacterial aggregates, and virulence of enteropathogenic Escherichia coli. Science 280, 2114-2118 (1998).
9. Comolli, J. C. et al. Pseudomonas aeruginosa gene products PilT and PilU are required for cytotoxicity in vitro and virulence in a mouse model of acute pneumonia. Infect. Immun. 67, 3625-3630 (1999).
10. Pujol, C., Eugene, E., Marceau, M. & Nassif, X. The meningococcal PilT protein is required for induction of intimate attachment to epithelial cells following pilus-mediated adhesion. Proc. Natl Acad. Sci. USA 96, 4017-4022 (1999).
11. Merz, A. J., Enns, C. A. & So, M. Type IV pili of pathogenic Neisseriae elicit cortical plaque formation in epithelial cells. Mol. Microbiol. 32, 1316-1332 (1999).
12. Seifert, H. S., Ajioka, R. S., Marchal, C., Sparling, P. F. & So, M. DNA transformation leads to pilin antigenic variation in Neisseria gonorrhoeae. Nature 336, 392-395 (1988).
13. Dubnau, D. DNA uptake in bacteria. Annu. Rev. Microbiol. 53, 217-244 (1999).
14. Yoshida, T., Kim, S. R. & Komano, T. Twelve pil genes are required for biogenesis of the R64 thin pilus. J. Bacteriol. 181, 2038-2043 (1999).
15. Bradley, D. E. Evidence for the retraction of Pseudomonas aeruginosa RNA phage pili. Biochem. Biophys. Res. Commun. 47, 142-149 (1972).
16. Karaolis, D. K., Somara, S., Maneval, D. R. Jr, Johnson, J. A. & Kaper, J. B. A bacteriophage encoding a pathogenicity island, a type-IV pilus and a phage receptor in cholera bacteria. Nature 399, 375-379 (1999).
17. Parge, H. E. et al. Structure of the fibre-forming protein pilin at 2.6 Å resolution. Nature 378, 32-38 (1995).
18. Forest, K. T. & Tainer J. A. Type-4 pilus structure: outside to inside and top to bottom - a minireview. Gene 192, 165-169 (1997).
19. Whitchurch, C. B., Hobbs, M., Livingston, S. P., Krishnapillai, V. & Maltick, J. S. Characterisation of a Pseudomonas aeruginosa twitching motility gene and evidence for a specialised protein export system widespread in eubacteria. Gene 101, 33-44 (1991).
20. Wolfgang, M. et al. pilT mutations lead to simultaneous defects in competence for natural transformation and twitching motility in piliated Neisseria gonorrhoeae. Mol. Microbiol. 29, 321-330 (1998).
21. Brossay, L., Paradis, G., Fox, R., Koomey, M. & Hebert, J. Identification, localization, and distribution of the PilT protein in Neisseria gonorrhoeae. Infect. Immun. 62, 2302-2308 (1994).
22. Krause, S. et al. Sequence-related protein export NTPases encoded by the conjugative transfer region of RP4 and by the cag pathogenicity island of Helicobacter pylori share similar hexameric ring structures. Proc. Natl Acad. Sci. USA 97, 3067-3072 (2000).
23. Novotny, C. P. & Fives-Taylor, P. Retraction of F pili. J. Bacteriol. 117, 1306-1311 (1974).
24. Ginocchio, C. C., Olmsted, S. B., Wells, C. L. & Galan, J. E. Contact with epithelial cells induces the formation of surface appendages on Salmonella typhimurium. Cell 76, 717-724 (1994).
25. Evans, E., Berk, D. & Leung, A. Detachment of agglutinin-bonded red blood cells. I. Forces to rupture molecular-point attachments. Biophys. J. 59, 838-848 (1991).
26. Shao, J. Y., Ting-Beall, H. P. & Hochmuth, R. M. Static and dynamic lengths of neutrophil microvilli. Proc. Natl Acad. Sci. USA 95, 6797-6802 (1998).
27. Coppin, C. M., Finer, I. T., Spudich, J. A. & Vale, R. D. Detection of sub-8-nm movements of kinesin by high-resolution optical-trap microscopy. Proc. Natl Acad. Sci. USA 93, 1913-1917 (1996).
28. Mahadevan, I., & Matsudaira, P. Motility powered by supramolecular springs and ratchets. Science 288, 95-100 (2000).
29. Dupuy, B., Taha, M. K., Pugsley, A. P. & Marchal, C. Neisseria gonorrhoeae prepilin export studied in Escherichia coli. J. Bacteriol. 173, 7589-7598 (1991).
30. Felsenfeld, D. P., Schwartzberg, P. L., Venegas, A., Tse, R. & Sheetz, M. P. Selective regulation of integrin-cytoskeleton interactions by the tyrosine kinase Src. Nature Cell Biol. 1, 200-206 (1999).