Differential expression of virulence genes and motility in Ralstonia (Pseudomonas) solanacearum during exponential growth

Applied and Environmental Microbiology

Volumn 63, Issue 3, 1997, Pages 844-850

  Clough, Steven J ^a,b   Flavier, Albert B ^a,c   Schell, Mark A ^a,d   Denny, Timothy P ^a  

^a UNIVERSITY OF GEORGIA   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^c Ciba Geigy Corporation   (United States)

^d UNIVERSITY OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GROWTH; BACTERIAL VIRULENCE; CELL CULTURE; CELL DENSITY; DNA BINDING; DNA FLANKING REGION; GENE CONTROL; GENE EXPRESSION; GENE FUSION; GENE MUTATION; PSEUDOMONAS; REPORTER GENE; SIGNAL TRANSDUCTION;

BACTERIA (MICROORGANISMS); PSEUDOMONAS; RALSTONIA SOLANACEARUM;

EID: 0031018796     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.63.3.844-850.1997     Document Type: Article

References (50)

1. Amsler, C. D., M. Cho, and P. Matsumura. 1993. Multiple factors underlying the maximum motility of Escherichia coli as cultures enter post-exponential growth. J. Bacteriol. 175:6238-6244.
2. Armitage, J. P. 1992. Bacterial motility and chemotaxis. Sci. Prog. 76:451-477.
3. Bassler, B. L., M. Wright, and M. R. Silverman. 1994. Multiple signalling systems controlling expression of luminescence in Vibrio harveyi: sequence and function of genes encoding a second sensory pathway. Mol. Microbiol. 13:273-286.
4. Beck von Bodman, S., and S. K. Farrand. 1995. Capsular polysaccharide biosynthesis and pathogenicity in Erwinia stewartii require induction by an N-acylhomoserine lactone autoinducer. J. Bacteriol. 177:5000-5008.
5. Bradbury, J. F. 1986. Guide to plant pathogenic bacteria. CAB International, Slough, United Kingdom.
6. Brumbley, S. M., B. F. Carney, and T. P. Denny. 1993. Phenotype conversion in Pseudomonas solanacearum due to spontaneous inactivation of PhcA, a putative LysR transcriptional regulator. J. Bacteriol. 175:5477-5487
7. Brumbley, S. M., and T. P. Denny. 1990. Cloning of wild-type Pseudomonas solanacearum phcA, a gene that when mutated alters expression of multiple traits that contribute to virulence. J. Bacteriol. 172:5677-5685.
8. Carney, B. F., and T. P. Denny. 1990. A cloned avirulence gene from Pseudomonas solanacearum determines incompatibility on Nicotiana tabacum at the host species level. J. Bacteriol. 172:4836-1843.
9. Clough, S. J., K.-E. Lee, M. A. Schell, and T. P. Denny. Unpublished data.
10. Clough, S. J., M. A. Schell, and T. P. Denny. 1994. Evidence for involvement of a volatile extracellular factor in Pseudomonas solanacearun virulence gene expression. Mol. Plant-Microb. Interact. 7:621-630.
11. Denny, T. P., and S. R. Baek. 1991. Genetic evidence that extracellular polysaccharide is a virulence factor of Pseudomonas solanacearum. Mol. Plant-Microb. Interact. 4:198-206.
12. Denny, T. P., S. M. Brumbley, B. F. Carney, S. J. Clough, and M. A. Schell. 1994. Phenotype conversion of Pseudomonas solanacearum, p. 137-143. In A. C. Hayward and G. L. Hartman (ed.), Bacterial wilt: the disease and its causative agent. Pseudomonas solanacearum. CAB International, Wallingford, United Kingdom.
13. Denny, T. P., L. M. Ganova-Raeva, J. Huang, and M. A. Schell. 1996. Cloning and characterization of tek, the gene encoding the major extracellular protein of Pseudomonas solanacearum. Mol. Plant-Microb. Interact. 8:272-281.
14. Denny, T. P., F. W. Makini, and S. M. Brumbley. 1988. Characterization of Pseudomonas solanacearum Tn5 mutants deficient in extracellular polysaccharide. Mol. Plant-Microb. Interact. 1:215-223.
15. Eberhard, A. 1972. Inhibition and activation of bacterial luciferase synthesis. J. Bacteriol. 109:1101-1105.
16. Eberhard, A., A. L. Burlingame, C. Eberhard, G. L. Kenyon, and N. J. Oppenheimer. 1981. Structural identification of autoinducer of Photobacterium fischeri luciferase. Biochemistry 20:2444-2449.
17. Flavier, A. B., and T. P. Denny. Unpublished data.
18. Flavier, A. B., and T. P. Denny. 1994. Purification and characterization of a volatile, endogenous factor that restores expression of virulence-associated genes in a Pseudomonas solanacearum mutant. Phytopathology 84:1134. (Abstract.)
19. Fuqua, W. C., S. C. Winans, and E. P. Greenberg. 1994. Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators. J. Bacteriol. 176:269-275.
20. Ganova-Raeva, L. M., A. B. Flavier, and T. P. Denny. 1994. Pseudomonas solanacearum produces a homoserine lactone-like signal molecule. Phytopathology 84:1134. (Abstract.)
21. Hayward, A. C. 1995. Pseudomonas solanacearum, p. 139-151. In U. S. Singh, R. P. Singh, and K. Kohmoto (ed.), Pathogenesis and host specificity in plant diseases: histopathological, biochemical, genetic and molecular bases, vol. I. Prokaryotes. Elsevier Science, Inc. Tarrytown, N.Y.
22. Hodgkiss, W. 1964. The flagella of Pseudomonas solanacearum. J. Appl. Bacteriol. 27:278-280.
23. Huang, J., B. F. Carney, T. P. Denny, A. K. Weissinger, and M. A. Schell. 1995. A complex network regulates expression of eps and other virulence genes of Pseudomonas solanacearum. J. Bacteriol. 177:1259-1267.
24. Huang, J., T. P. Denny, and M. A. Schell. 1993. VsrB, a regulator of virulence genes of Pseudomonas solanacearum, is homologous to sensors of the two-component family. J. Bacteriol. 175:6169-6178.
25. Huang, J., and M. A. Schell. Unpublished data.
26. 25a. Hwang, I. Y., D. M. Cook, and S. K. Farrand. 1995. A new regulatory element modulates homoserine lactone-mediated autoinduction of Ti plasmid conjugal transfer. J. Bacteriol. 177:449-458.
27. Kaiser, D., and R. Losick. 1993. How and why bacteria talk to each other. Cell 73:873-885.
28. Kaplan, H. B., and E. P. Greenberg. 1985. Diffusion of autoinducer is involved in regulation of the Vibrio fischeri luminescence system. J. Bacteriol. 163:1210-1214.
29. Kelman, A., and J. Hruschka. 1973. The role of motility and aerotaxis in the selective increase of avirulent bacteria in still broth cultures of Pseudomonas solanacearum. J. Gen. Microbiol. 76:177-188.
30. Klotz, M. G. 1993. The importance of bacterial growth phase for in planta virulence and pathogenicity testing: coordinated stress response regulation in fluorescent pseudomonads? Can. J. Microbiol. 39:948-957.
31. Kolter, R., D. A. Siegele, and A. Tormo. 1993. The stationary phase of the bacterial life cycle. Annu. Rev. Microbiol. 47:855-874.
32. s. J. Bacteriol. 173:4474-4481.
33. Macnab, R. M. 1987. Flagella, p. 70-83. In F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
34. Maniatis, T., E.V. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
35. Mead, D. A., E. S. Skorupa, and B. Kemper. 1985. Single stranded DNA promoter plasmids for engineering mutant RNA's and protein: synthesis of a 'stretched' parathyroid hormone. Nucleic Acids Res. 13:1103-1108.
36. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
37. Orgambide, G., H. Montrozier, P. Servin, J. Roussel, D. Trigalet-Demery, and A. Trigalet. 1991. High heterogeneity of the exopolysaccharides of Pseudomonas solanacearum strain GMI1000 and the complete structure of the major polysaccharide. J. Biol. Chem. 266:8312-8321.
38. Piper, K. R., S. Beck von Bodman, and S. K. Farrand. 1993. Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction. Nature 362:448-450.
39. Pirhonen, M., D. Flego, R. Heikinheimo, and E. T. Palva. 1993. A small diffusible signal molecule is responsible for the global control of virulence and exoenzyme production in the plant pathogen Erwinia carotovora. EMBO 1267:2467-2476.
40. Roberts, D. P., T. P. Denny, and M. A. Schell. 1988. Cloning of the egl gene of Pseudomonas solanacearum and analysis of its role in phytopathogenicity. J. Bacteriol. 170:1445-1451.
41. Salmond, G. P. C., B. W. Bycroft, G. S. A. B. Stewart, and P. Williams. 1995. The bacterial 'enigma': cracking the code of cell-cell communication. Mol. Microbiol. 16:615-624.
42. Schell, M. A. 1993. Molecular biology of the LysR family of transcriptional regulators. Annu. Rev. Microbiol. 47:597-626.
43. Schell, M. A. 1996. To be or not to be: how Pseudomonas solanacearum decides whether or not to express virulence genes. Eur. J. Plant. Pathol. 102:459-469.
44. Schell, M. A., T. P. Denny, and J. Huang. 1994. VsrA, a second two-component sensor regulating virulence genes of Pseudomonas solanacearum. Mol. Microbiol. 11:489-500.
45. Schell, M. A., T. P. Denny, and J. Huang. 1994. Extracellular virulence factors of Pseudomonas solanacearum: role in disease and their regulation, p. 311-324. In C. I. Kado and J. H. Crosa (ed.), Molecular mechanisms of bacterial virulence. Kluwer Academic, Dordrecht, The Netherlands.
46. Simon, R., J. Quandt, and W. Klipp. 1989. New derivatives of transposon Tn5 suitable for mobilization of replicons, generation of operon fusions and induction of genes in Gram-negative bacteria. Gene 80:161-169.
47. Smith, E. F. 1914. Wilt-diseases of tobacco, p. 220-281. In Bacteria in relation to plant diseases, vol. III. Carnegie Institute of Washington, Washington, D.C.
48. Vaituzis, Z., and R. N. Doetsch. 1969. Motility tracks: technique for quantitative study of bacterial movement. J. Appl. Bacteriol. 17:584-588.
49. Weichart, D., R. Lange, N. Henneberg, and R. Hengge-Aronis. 1993. Identification and characterization of stationary phase-inducible genes in Escherichia coli. Mol. Microbiol. 10:407-420.
50. Yabuuchi, E., Y. Kosako, I. Yano, H. Hotta, and Y. Nishiuchi. 1995. Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov.: proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. nov., Ralstonia solanacearum (Smith 1896) comb. nov. and Ralstonia eutroplia (Davis 1969) comb. nov. Microbiol. Immunol. 39:897-904.