The comP locus of Neisseria gonorrhoeae encodes a type IV prepilin that is dispensable for pilus biogenesis but essential for natural transformation

Molecular Microbiology

Volumn 31, Issue 5, 1999, Pages 1345-1357

  Wolfgang, Matthew ^a   Van Putten, Jos P M ^b   Hayes, Stanley F ^c   Koomey, Michael ^a,d  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

^c NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^d UNIVERSITY OF OSLO   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN;

ARTICLE; BACTERIUM PILUS; BIOGENESIS; CELL AGGLUTINATION; DNA TRANSFER; EPITHELIUM CELL; GENE DISRUPTION; GENE LOCUS; GENETIC TRANSFORMATION; HUMAN; HUMAN CELL; NEISSERIA GONORRHOEAE; NONHUMAN; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; NEISSERIA GONORRHOEAE;

EID: 0032982528     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1999.01269.x     Document Type: Article

References (39)

1. Alm, R.A., and Mattick, J.S. (1995) Identification of a gene, pilV, required for type 4 fimbrial biogenesis in Pseudomonas aeruginosa, whose product possesses a prepilin-like leader sequence. Mol Microbiol 16: 485-496.
2. Alm, R.A., and Mattick, J.S. (1997) Genes involved in the biogenesis and function of type-4 fimbriae in Pseudomonas aeruginosa. Gene 192: 89-98.
3. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. (1990) Basic local alignment search tool. J Mol Biol 215: 403-410.
4. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein data-base search programs. Nucleic Acids Res 25: 3389-3402.
5. Biswas, G.D., Sox, T., Blackman, E., and Sparling, P.F. (1977) Factors affecting genetic transformation of Neisseria gonorrhoeae. J Bacteriol 129: 983-992.
6. Bradley, D.E. (1980) A function of Pseudomonas aeruginosa PAO polar pili: twitching motility. Can J Microbiol 26: 146-154.
7. Catlin, B.W. (1982) Cell-to-cell transmission of chromosomal loci in Neisseria gonorrhoeae. In Genetic Exchange. Streips, U.N., Guild, W.R., Goodgal, S.H., and Wilson, G.A. (eds). New York: Marcel Dekker, pp. 311-321.
8. Darzins, A. (1993) The pilG gene product, required for Pseudomonas aeruginosa pilus production and twitching motility, is homologous to the enteric single-domain response regulator CheY. J Bacteriol 175: 5934-5944.
9. Drake, S.L., and Koomey, M. (1995) The product of the pilQ gene is essential for the biogenesis of type IV pili in Neisseria gonorrhoeae. Mol Microbiol 18: 975-986.
10. Dubnau, D. (1991) Genetic competence in Bacillus subtilis. Microbiol Rev 55: 395-424.
11. Dubnau, D. (1997) Binding and transport of transforming DNA by Bacillus subtilis: the role of type-IV pilin-like proteins - a review. Gene 192: 191-198.
12. Francetic, O., Lory, S., and Pugsley, A.P. (1998) A second prepilin peptidase gene in Escherichia coli K-12. Mol Microbiol 27: 763-775.
13. Freitag, N., Seifert, H.S., and Koomey, M. (1995) Characterization of the pilF-pilD pilus assembly locus of Neisseria gonorrhoeae. Mol Microbiol 16: 575-586.
14. Gunn, J.S., and Stein, D.C. (1996) Use of a non-selective transformation technique to construct a multiply restriction/ modification-deficient mutant of Neisseria gonorrhoeae. Mol Gen Genet 251: 509-517.
15. Inamine, G.S., and Dubnau, D. (1995) ComEA, a Bacillus subtilis integral membrane protein required for genetic transformation, is needed for both DNA binding and transport. J Bacteriol 177: 3045-3051.
16. Kim, S.R., and Komano, T. (1997) The plasmid R64 thin pilus identified as a type IV pilus. J Bacteriol 179: 3594-3603.
17. Koomey, M., Bergstrom, S., Blake, M., and Swanson, J. (1991) Pilin expression and processing in pilus mutants of Neisseria gonorrhoeae: critical role of Gly, in assembly. Mol Microbiol 5: 279-287.
18. Link, C., Eickernjager, S., Porstendorfer, D., and Averhoff, B. (1998) Identification and characterization of a novel competence gene, comC, required for DNA binding and uptake in Acinetobacter sp. strain BD413. J Bacteriol 180: 1592-1595.
19. Lu, H.M., Motley, S.T., and Lory, S. (1997) Interactions of the components of the general secretion pathway: role of Pseudomonas aeruginosa type IV pilin subunits in complex formation and extracellular protein secretion. Mol Microbiol 25: 247-259.
20. Nunn, D.N., and Lory, S. (1993) Cleavage, methylation, and localization of the Pseudomonas aeruginosa export proteins XcpT, -U, -V, and -W. J Bacteriol 175: 4375-4382.
21. Porstendorfer, D., Drotschmann, U., and Averhoff, B. (1997) A novel competence gene, comP, is essential for natural transformation of Acinetobacter sp. strain BD413. Appl Env Microbiol 63: 4150-4157.
22. Pugsley, A.P. (1993a) The complete general secretory pathway in Gram-negative bacteria. Microbiol Rev 57: 50-108.
23. Pugsley, A.P. (1993b) Processing and methylation of PulG, a pilin-like component of the general secretory pathway of Klebsiella oxytoca. Mol Microbiol 9: 295-308.
24. Pugsley, A.P., Francetic, O., Possot, O.M., Sauvonnet, N., and Hardie, K.R. (1997) Recent progress and future directions in studies of the main terminal branch of the general secretory pathway in Gram-negative bacteria - a review. Gene 192: 13-19.
25. van Putten, J.P.M., and Paul, S.M. (1995) Binding of syndecan-like cell surface proteoglycan receptors is required for Neisseria gonorrhoeae entry into human mucosal cells. EMBO J 14: 2144-2154.
26. Rudel, T., Facius, D., Barten, R., Scheuerpflug, I., Nonnenmacher, E., and Meyer, T.F. (1995) Role of pili and the phase-variable PilC protein in natural competence for transformation of Neisseria gonorrhoeae. Proc Natl Acad Sci USA 92: 7986-7990.
27. Sambrook, J., Fritsch, E.J., and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, New York: Cold Spring, Harbor Laboratory Press.
28. Seifert, H. (1997) Insertionally inactivated and inducible recA alleles for use in Neisseria. Gene 188: 215-220.
29. Seifert, H.S., Ajioka, R.S., Paruchuri, D., Heffron, F., and So, M. (1990) Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence. J Bacteriol 172: 40-46.
30. Stein, D.C., Danaher, R.J., and Cook, T.M. (1991) Characterization of a gyrB mutation responsible for low-level nalidixic acid resistance in Neisseria gonorrhoeae. Antimicrob Agents Chemother 35: 622-626.
31. Strom, M.S., and Lory, S. (1991) Amino acid substitutions in pilin of Pseudomonas aeruginosa. Effect on leader peptide cleavage, amino-terminal methylation, and pilus assembly. J Biol Chem 266: 1656-1664.
32. Swanson, J. (1978) Studies on gonococcus infection XII. Colony color and opacity variants of gonococci. Infect Immun 19: 320-331.
33. Swanson, J., and Barrera, O. (1983) Gonococcal pilus sub-unit size heterogeneity correlates with transitions in colony piliation phenotype, not with changes in colony opacity. J Exp Med 158:1459-1472.
34. Tabor, S., and Richardson, C.C. (1985) A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci USA 82: 1074-1078.
35. Tomb, J., El-Hajj, H., and Smith, H.O. (1991) Nucleotide sequence of a cluster of genes involved in the transformation of Haemophilus influenzae Rd. Gene 104: 1-10.
36. Tønjum, T., Freitag, N.E., Normark, E., and Koomey, M. (1995) Identification and characterization of pilG, a highly conserved pilus assembly gene in pathogenic Neisseria. Mol Microbiol 16:451-464.
37. Waldor, M.K., and Mekalanos, J.J. (1996) Lysogenic conversion by a filamentous phage encoding cholera toxin. Science 272: 1910-1914.
38. Watts, T.H., Kay, C.M., and Paranchych, W. (1983) Spectral properties of three quaternary arrangements of Pseudomonas pilin. Biochem 22: 3640-3646.
39. Wolfgang, M., Lauer, P., Park, H.P., Brossay, L., Hebert, J., and Koomey, M. (1998) PilT mutations lead to simultaneous defects in competence for natural transformation and twitching motility in piliated Neisseria gonorrhoeae. Mol Microbiol 29:321-330.