Rac-PAK signaling stimulates extracellular signal-regulated kinase (ERK) activation by regulating formation of MEK1-ERK complexes

Molecular and Cellular Biology

Volumn 22, Issue 17, 2002, Pages 6023-6033

  Eblen, Scott T ^a   Slack, Jill K ^a   Weber, Michael J ^a   Catling, Andrew D ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; P21 ACTIVATED KINASE; PROTEIN; PROTEIN CDC42; PROTEIN P21; RAC PROTEIN; UNCLASSIFIED DRUG;

ADHESION; ANIMAL CELL; ARTICLE; CELL STRAIN COS1; COMPLEX FORMATION; ENZYME ACTIVATION; ENZYME PHOSPHORYLATION; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CELL ADHESION; CERCOPITHECUS AETHIOPS; COS CELLS; CULTURE MEDIA, SERUM-FREE; ENZYME ACTIVATION; EPIDERMAL GROWTH FACTOR; MACROMOLECULAR SUBSTANCES; MAP KINASE KINASE 1; MAP KINASE SIGNALING SYSTEM; MICE; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE KINASES; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STRUCTURE, TERTIARY; PROTEIN-SERINE-THREONINE KINASES; PROTO-ONCOGENE PROTEINS P21(RAS); RAC GTP-BINDING PROTEINS; RECOMBINANT FUSION PROTEINS; SEQUENCE DELETION; TRANSFECTION;

ANIMALIA;

EID: 0036333485     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.22.17.6023-6033.2002     Document Type: Article

References (51)

1. Integrin and cytoskeletal regulation of growth factor signaling to the MAP kinase pathway
2. Integrin-mediated adhesion regulates ERK nuclear translocation and phosphorylation of Elk-1
3. A conserved docking site in MEKS mediates high-affinity binding to MAP kinases and cooperates with a scaffold protein to enhance signal transmission
4. Signaling in the yeast pheromone response pathway: Specific and high-affinity interaction of the mitogen-activated protein (MAP) kinases Kss1 and Fus3 with the upstream MAP kinase kinase Ste7
5. Identification of MAP kinase domains by redirecting stress signals into growth factor responses
6. Nuclear translocation of p42/p44 mitogen-activated protein kinase is required for growth factor-induced gene expression and cell cycle entry
7. Increasing complexity of Ras signaling
8. Scaffold protein regulation of mitogen-activated protein kinase cascade
9. A proline-rich sequence unique to MEK1 and MEK2 is required for Raf binding and regulates MEK function
10. Nuclear localization and regulation of erk- and rsk-encoded protein kinases
11. PAK1 primes MEK1 for phosphorylation by Raf-1 kinase during cross-cascade activation of the ERK pathway
12. The small GTP-binding proteins Rac1 and Cdc42 regulate the activity of the JNK/SAPK signaling pathway
13. Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells
14. The MEK1 proline-rich insert is required for efficient activation of the mitogen-activated protein kinases ERK1 and ERK2 in mammalian cells
15. Adhesion to the extracellular matrix regulates the coupling of the small GTPase Rac to its effector PAK
16. Biochemical and biological functions of the N-terminal, noncatalytic domain of extracellular signal-regulated kinase 2
17. Tripping the switch fantastic: How a protein kinase cascade can convert graded inputs into switch-like outputs
18. Cross-cascade activation of ERKs and ternary complex factors by Rho family proteins
19. Actions of Rho family small G proteins and p21-activated protein kinases on mitogen-activated protein kinase family members
20. Cytoplasmic localization of mitogen-activated protein kinase kinase directed by its NH2-terminal, leucine-rich short amino acid sequence, which acts as a nuclear export signal
21. Interaction of MAP kinase with MAP kinase kinase: Its possible role in the control of nucleocytoplasmic transport of MAP kinase
22. Embryonic death of Mek1-deficient mice reveals a role for this kinase in angiogenesis in the labyrinthine region of the placenta
23. Serum-induced translocation of mitogen-activated protein kinase to the cell surface ruffling membrane and the nucleus
24. Dual phosphorylation and autophosphorylation in mitogen-activated protein (MAP) kinase activation
25. RAS and RAF-1 form a signalling complex with MEK-1 but not MEK-2
26. Phosphorylation of the MAP kinase ERK2 promotes its homodimerization and nuclear translocation
27. The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338
28. Requirement for Ras in Raf activation is overcome by targeting Raf to the plasma membrane
29. Growth factors induce nuclear translocation of MAP kinases (p42mapk and p44mapk) but not of their activator MAP kinase kinase (p45mapkk) in fibroblasts
30. Cell anchorage permits efficient signal transduction between ras and its downstream kinases
31. Transformation of mammalian cells by constitutively active MAP kinase kinase
32. Selective activation of the JNK signaling cascade and c-Jun transcriptional activity by the small GTPases Rac and Cdc42Hs
33. Integrins can collaborate with growth factors for phosphorylation of receptor tyrosine kinases and MAP kinase activation: Roles of integrin aggregation and occupancy of receptors
34. Complexes of Ras GTP with Raf-1 and mitogen-activated protein kinase kinase
35. The MAP kinase cascade is essential for diverse signal transduction pathways
36. Mitogen-activated protein kinases p42mapk and p44mapk are required for fibroblast proliferation
37. Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase)
38. Growth factor activation of MAP kinase requires cell adhesion
39. Identification of novel point mutations in ERK2 that selectively disrupt binding to MEK1
40. Alpha5beta1 integrin controls cyclin D1 expression by sustaining mitogen-activated protein kinase activity in growth factor-treated cells
41. Identification of a cytoplasmic-retention sequence in ERK2
42. MP1: A MEK binding partner that enhances enzymatic activation of the MAP kinase cascade
43. p21-activated kinase 1 (PAK1) regulates cell motility in mammalian fibroblasts
44. Integrins regulate the linkage between upstream and downstream events in G protein-coupled receptor signaling to mitogen-activated protein kinase
45. Activation of Raf as a result of recruitment to the plasma membrane
46. A conserved docking motif in MAP kinases common to substrates, activators and regulators
47. Timing of cyclin D1 expression within G1 phase is controlled by Rho
48. Phosphorylation of MAP kinases by MAPK/ERK kinase involves multiple regions of MAP kinases
49. Hydrophobic as well as charged residues in both mek1 and erk2 are important for their proper docking
50. The N-terminal ERK-binding site of MEK1 is required for efficient feedback phosphorylation by ERK2 in vitro and ERK activation in vivo
51. Active MAP kinase in mitosis: Localization at kinetochores and association with the motor protein CENP-E