Constitutive and regulated expression of processed insulin following in vivo hepatic gene transfer

Gene Therapy

Volumn 9, Issue 14, 2002, Pages 963-971

  Auricchio, A ^a   Gao, G P ^a   Yu, Q C ^a   Raper, S ^a   Rivera, V M ^b   Clackson, T ^b   Wilson, J M ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b ARIAD PHARMACEUTICALS INC   (United States)

Author keywords

Adenoviral vector; Furin modified proinsulin; Liver; Pro hormone convertases; Rapamycin inducible transcription; Regulated secretion

Indexed keywords

ADENOVIRUS VECTOR; FURIN; GLUCOSE; INSULIN; PROINSULIN; RAPAMYCIN; STREPTOZOCIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DIMERIZATION; DRUG EFFECT; DRUG WITHDRAWAL; FEMALE; GENE TRANSFER; GENETIC CODE; GENETIC ENGINEERING; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; IN VIVO STUDY; INSULIN DEPENDENT DIABETES MELLITUS; INSULIN RELEASE; LIVER CELL; MOUSE; NONHUMAN; PANCREAS ISLET CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PROCESSING; WILD TYPE;

ADENOVIRIDAE; ANIMALS; CELLS, CULTURED; COMBINED MODALITY THERAPY; DIABETES MELLITUS, EXPERIMENTAL; DIABETES MELLITUS, TYPE 1; DIMERIZATION; FEMALE; GENE EXPRESSION; GENE THERAPY; GENETIC ENGINEERING; GENETIC VECTORS; HEPATOCYTES; INSULIN; MICE; MICE, NUDE; PROINSULIN; SIROLIMUS; TRANSFECTION;

ADENOVIRIDAE; ANIMALIA; MURINAE;

EID: 0036328505     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301746     Document Type: Article

References (45)

1. Diabetes mellitus
2. Principles of insulin therapy
3. Gene and cell-replacement therapy in the treatment of type 1 diabetes: How high must the standards be set?
4. Insulin biosynthesis and chemistry
5. Proinsulin processing in the regulated and the constitutive secretory pathway
6. The Role of Glucose Transport and Phosphorylation in Glucose-Stimulated Insulin Secretion
7. Hepatic insulin production for type 1 diabetes
8. Sustained expression of genes delivered directly into liver and muscle by lentiviral vectors
9. Recombinant adeno-associated virus for muscle directed gene therapy
10. Persistent and therapeutic concentrations of human factor IX in mice after hepatic gene transfer of recombinant AAV vectors
11. Liver specification and early morphogenesis
12. The genes of hepatic glucose metabolism
13. KATP channels regulate mitogenically induced proliferation in primary rat hepatocytes and human liver cell lines. Implications for liver growth control and potential therapeutic targeting
14. Gene therapy of diabetes: Glucose-stimulated insulin secretion in a human hepatoma cell line (HEP G2ins/g)
15. The IRI release from insulin gene-transduced hepatic cells responds to ambient glucose concentration
16. A humanized system for pharmacologic control of gene expression
17. Proinsulin conversion in GH3 cells after coexpression of human proinsulin with the endoproteases PC2 and/or PC3
18. Genetically engineered proinsulin constitutively processed and secreted as mature, active insulin
19. Regulation of protein secretion through controlled aggregation in the endoplasmic reticulum
20. Engineering of a glucose-responsive surrogate cell for insulin replacement therapy of experimental insulin-dependent diabetes
21. Engineering a glucose-responsive human insulin-secreting cell line from islets of Langerhans isolated from a patient with persistent hyperinsulinemic hypoglycemia of infancy
22. Evidence for expression of the facilitated glucose transporter in rat hepatocytes
23. Gene therapy for diabetes mellitus in rats by hepatic expression of insulin
24. Adenovirus-mediated transfer of a modified human proinsulin gene reverses hyperglycemia in diabetic mice
25. Remission in models of type 1 diabetes by gene therapy using a single-chain insulin analogue
26. Auto-regulated hepatic insulin gene expression in type 1 diabetic rats
27. Hepatic insulin expression improves glycemic control in type 1 diabetic rats
28. Regulated hepatic insulin gene therapy of STZ-diabetic rats
29. Proinsulin processing in the rat insulinoma cell line INS after overexpression of the endoproteases PC2 or PC3 by recombinant adenovirus
30. Proinsulin processing by the subtilisin-related proprotein convertases furin, PC2 and PC3
31. Processing of proinsulin by furin, PC2 and PC3 in (co) transfected COS (monkey kidney) cells
32. Streptozotocin-induced pancreatic insulitis: New model of diabetes mellitus
33. The role of prohormone convertases in insulin biosynthesis: Evidence for inherited defects in their action in man and experimental animals
34. Glucose-dependent insulin release from genetically engineered K cells
35. Diabetes. Gene therapy for rats and mice (news)
36. Pancreatic and duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced hyperglycemia
37. Recent advances in inducible gene expression systems
38. Regulated gene expression systems
39. Long-term regulated expression of growth hormone in mice after intramuscular gene transfer
40. Regulated delivery of therapeutic proteins after in vivo somatic cell gene transfer
41. High throughput method for creating and screening recombinant adenoviruses
42. Biology of adenovirus vectors with E1 and E4 deletions for liver-directed gene therapy
43. Immuno-affinity purification
44. Epidermal growth factor induces protein tyrosine phosphorylation and association of p190 with ras-GTP-ase activating protein in Caco-2 cells
45. Mice expressing a mutant desmosomal cadherin exhibit abnormalities in desmosomes, proliferation, and epidermal differentiation