Identification of the catalytic subdomain of the VS ribozyme and evidence for remarkable sequence tolerance in the active site loop

Journal of Molecular Biology

Volumn 320, Issue 3, 2002, Pages 443-454

  Sood, Vanita D ^a   Collins, Richard A ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

Active site; Chemical modification; Deletion; Kinetics; Ribozyme

Indexed keywords

RIBOZYME;

ALPHA HELIX; ARTICLE; CATALYSIS; CATALYST; CONTROLLED STUDY; GENE DELETION; NEUROSPORA; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN STRUCTURE; SEQUENCE HOMOLOGY;

NEUROSPORA;

EID: 0036314853     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(02)00521-1     Document Type: Article

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16. A secondary-structure model for the self-cleaving region of Neurospora VS RNA
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18. Rearrangement of a stable RNA secondary structure during VS ribozyme catalysis
19. A long-range pseudoknot is required for activity of the Neurospora VS ribozyme
20. Intramolecular secondary structure rearrangement by the kissing interaction of the Neurospora VS ribozyme
21. A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA
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26. Rapid formation of a solvent-inaccessible core in the Neurospora Varkud satellite ribozyme
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28. The A730 loop is an important component of the active site of the VS ribozyme
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31. Crystal structure of a group I ribozyme domain: Principles of RNA packing
32. A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing
33. GAAA tetraloop and conserved bulge stabilize tertiary structure of a group I intron domain
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36. An unusual pH-independent and metal-ion-independent mechanism for hairpin ribozyme catalysis
37. Metal ions play a passive role in the hairpin ribozyme catalysed reaction
38. Hairpin ribozyme cleavage catalyzed by aminoglycoside antibiotics and the polyamine spermine in the absence of metal ions
39. The hammerhead, hairpin and VS ribozymes are catalytically proficient in monovalent cations alone
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44. A tyrosyl-tRNA synthetase can function similarly to an RNA structure in the Tetrahymena ribozyme
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47. Stability of hairpin ribozyme tertiary structure is governed by the interdomain junction
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49. Minimal sequence requirements for ribozyme activity
50. Properties of a hammerhead ribozyme with deletion of stem II
51. The contribution of 2′-hydroxyls to the cleavage activity of the Neurospora VS ribozyme
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53. Reaction conditions and kinetics of self-cleavage of a ribozyme derived from Neurospora VS RNA
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