A tyrosine-phosphorylated 12-amino-acid sequence of enteropathogenic Escherichia coli Tir binds the host adaptor protein Nck and is required for Nck localization to actin pedestals

Molecular Microbiology

Volumn 43, Issue 5, 2002, Pages 1227-1241

  Campellone, Kenneth G ^a   Giese, Andrew ^a   Tipper, Donald J ^a   Leong, John M ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; INTIMIN; PROTEIN; PROTEIN NCK; PROTEIN TIR; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL COLONIZATION; BACTERIAL OUTER MEMBRANE; CELL MEMBRANE; CHIMERA; CONTROLLED STUDY; ESCHERICHIA COLI; INTESTINE EPITHELIUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION;

ACTINS; AMINO ACID SEQUENCE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MOLECULAR SEQUENCE DATA; ONCOGENE PROTEINS; PHOSPHORYLATION; PHOSPHOTYROSINE; PROTEIN BINDING; RECEPTORS, CELL SURFACE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA; NEGIBACTERIA;

EID: 0036223873     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2002.02817.x     Document Type: Article

References (49)

1. Enteropathogenic Escherichia coli translocated intimin receptor, Tir, requires a specific chaperone for stable secretion
2. The Wiskott-Aldrich syndrome protein-interacting protein (WIP) binds to the adaptor protein Nck
3. Transcriptional regulation of the esp genes of enterohemorrhagic Escherichia coli
4. Agents that inhibit Rho, Rac, and Cdc42 do not block formation of actin pedestals in HeLa cells infected with enteropathogenic Escherichia coli
5. Membrane-targeting of signalling molecules by SH2/SH3 domain-containing adaptor proteins
6. EspE, a novel secreted protein of attaching and effacing bacteria, is directly translocated into infected host cells, where it appears as a tyrosine-phosphorylated 90 kDa protein
7. Enterohemorrhagic Escherichia coli O157:H7 produces Tir, which is translocated to the host cell membrane but is not tyrosine phosphorylated
8. Enterohaemorrhagic and enteropathogenic Escherichia coli use a different Tir-based mechanism for pedestal formation
9. Role of the eaeA gene in experimental enteropathogenic Escherichia coli infection
10. Small GTP-binding proteins of the Rho- and Ras-subfamilies are not involved in the actin rearrangements induced by attaching and effacing Escherichia coli
11. The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69
12. Identification of CesT, a chaperone for the type III secretion of Tir in enteropathogenic Escherichia coli
13. The cloned locus of enterocyte effacement from enterohemorrhagic Escherichia coli O157:H7 is unable to confer the attaching and effacing phenotype to E. coli K-12
14. Interaction of the enteropathogenic Escherichia coli protein, translocated intimin receptor (Tir), with focal adhesion proteins
15. Surfing pathogens and the lessons learned for actin polymerization
16. Actin-based motility of vaccinia virus mimics receptor tyrosine kinase signaling
17. The complete DNA sequence of vaccinia virus
18. Enteropathogenic E. coli translocated intimin receptor, Tir, interacts directly with alpha-actinin
19. Recruitment of cytoskeletal and signaling proteins to enteropathogenic and enterohemorrhagic Escherichia coli pedestals
20. Identification of the intimin-binding domain of Tir of enteropathogenic Escherichia coli
21. Enteropathogenic E. coli Tir binds Nck to initiate actin pedestal formation in host cells
22. Binding of intimin from enteropathogenic Escherichia coli to Tir and to host cells
23. Signal transduction responses following adhesion of verocytotoxin-producing Escherichia coli
24. A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells
25. Construction of vectors with the p15a replicon, kanamycin resistance, inducible lacZalpha and pUC18 or pUC19 multiple cloning sites
26. Enteropathogenic E. coli acts through WASP and Arp2/3 complex to form actin pedestals
27. Phosphorylation of tyrosine 474 of the enteropathogenic Escherichia coli (EPEC) Tir receptor molecule is essential for actin nucleating activity and is preceded by additional host modifications
28. The enterohaemorrhagic Escherichia coli (serotype O157:H7) Tir molecule is not functionally interchangeable for its enteropathogenic E. coli (serotype O127:H6) homologue
29. Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells
30. Enteropathogenic Escherichia coli protein secretion is induced in response to conditions similar to those in the gastrointestinal tract
31. The Tir-binding region of enterohaemorrhagic Escherichia coli intimin is sufficient to trigger actin condensation after bacterial-induced host cell signalling
32. Actin pedestal formation by enteropathogenic Escherichia coli and intracellular motility by Shigella flexneri are abolished in N-WASP-defective cells
33. The Nck SH2/SH3 adaptor protein: A regulator of multiple intracellular signal transduction events
34. A cloned pathogenicity island from enteropathogenic Escherichia coli confers the attaching and effacing phenotype on E. coli K-12
35. A complex of N-WASP and WIP integrates signalling cascades that lead to actin polymerization
36. How WASP-family proteins and the Arp2/3 complex convert intracellular signals into cytoskeletal structures
37. PCR-mediated gene replacement in Escherichia coli
38. Diarrheagenic Escherichia coli
39. Mechanism of actin-based motility
40. Signaling through scaffold, anchoring, and adaptor proteins
41. Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7
42. WIP, a protein associated with Wiskott-Aldrich syndrome protein, induces actin polymerization and redistribution in lymphoid cells
43. Hemorrhagic colitis associated with a rare Escherichia coli serotype
44. Wiscott-Aldrich syndrome protein physically associates with Nck through Src homology 3 domains
45. Nck and phosphatidylinositol 4,5-bisphosphate synergistically activate actin polymerization through the N-WASP-Arp2/3 pathway
46. A pathogenic bacterium triggers epithelial signals to form a functional bacterial receptor that mediates actin pseudopod formation
47. The eae gene of Citrobacter freundii biotype 4280 is necessary for colonization in transmissible murine colonic hyperplasia
48. Recognition and specificity in protein tyrosine kinase-mediated signalling
49. The role of the eaeA gene in diarrhea and neurological complications in a gnotobiotic piglet model of enterohemorrhagic Escherichia coli infection