Identification of CesT, a chaperone for the type III secretion of Tir in enteropathogenic Escherichia coli

Molecular Microbiology

Volumn 33, Issue 6, 1999, Pages 1176-1189

  Elliott, Simon J ^a   Hutcheson, Steven W ^a,b   Dubois, Maria S ^a   Mellies, Jay L ^a   Wainwright, Leslie A ^a   Batchelor, Miranda ^c   Frankel, Gad ^c   Knutton, Stuart ^d   Kaper, James B ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF MARYLAND   (United States)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

^d UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CHAPERONE; CYTOPLASM PROTEIN; VIRULENCE FACTOR;

ARTICLE; BACTERIUM MUTANT; CONTROLLED STUDY; ESCHERICHIA COLI; IMMUNOPRECIPITATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN SECRETION;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; CONSERVED SEQUENCE; DNA PRIMERS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES, BACTERIAL; MOLECULAR CHAPERONES; MOLECULAR SEQUENCE DATA; MUTATION; PHENOTYPE; PROTEIN BINDING; RECEPTORS, CELL SURFACE; SEQUENCE HOMOLOGY, AMINO ACID; SHIGELLA; TRANSFORMATION, GENETIC; VIRULENCE;

EID: 0032877318     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1999.01559.x     Document Type: Article

References (47)

1. Abe, A., de Grado, M., Pfuetzner, R.A., Sánchez-SanMartín, C., DeVinney, R., Puente, J.L., et al. (1999) Enteropathogenic Escherichia coli translocated intimin receptor, Tir, requires a specific chaperone for stable secretion. Mol Microbiol 33:
2. Anderson, D.M., and Schneewind, O. (1997) A mRNA signal for the type III secretion of Yop proteins by Yersinia enterocolitica. Science 278: 1140-1143.
3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (1994) Current Protocols in Molecular Biology. New York: John Wiley & Sons.
4. Bartel, P.I., Chien, C.T., Sternglanz, R., and Fields, S. (1994) Using the two-hybrid system to detect protein-protein interactions. In: Cellular Interactions in Development: a Practical Approach. Hartley, D.A. (ed.) Oxford: Oxford University Press, pp. 153-179.
5. Cheng, L.W., Anderson, D.M., and Schneewind, O. (1997) Two independent type III secretion mechanisms for YopE in Yersinia enterocolitica. Mol Microbiol 24: 757-765.
6. Clark, K.L., Larsen, P.B., Wang, X., and Chang, C. (1998) Association of the Arabidopsis CTR1 Raf-like kinase with the ETR1 and ERS ethylene receptors. Proc Natl Acad Sci USA 95: 5401-5406.
7. Coligan, J.E., Dunn, B.M., Ploegh, H.L., Speicher, D.W., and Wingfield, P.T. (1998) Current Protocols in Protein Science. New York: John Wiley & Sons.
8. Cornelis, G.R., Boland, A., Boyd, A.P., Geuijen, C., Iriarte, M., Neyt, C., Sory, M.P., and Stainier, I. (1998) The virulence plasmid of Yersinia, an antihost genome. Microbiol Mol Biol Rev 62: 1315-1352.
9. Elliott, S.J., and Kaper, J.B. (1997) Role of type 1 fimbriae in EPEC infections. Microb Pathog 23: 113-118.
10. Elliot, S., Wainwright, L.A., McDaniel, T., MacNamara, B., Donnenberg, M., and Kaper, J.B. (1998) The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69. Mol Microbiol 28: 1-4.
11. Frankel, G., Phillips, A.D., Rosenshine, I., Dougan, G., Kaper, J.B., and Knutton, S. (1998) Enteropathogenic and enterohaemorrhagic Escherichia coli: more subversive elements. Mol Microbiol 30: 911-921.
12. Frithz-Lindsten, E., Rosqvist, R., Johansson, L., and Forsberg, Å. (1995) The chaperone-like protein YerA of Yersinia pseudotuberculosis stabilizes YopE in the cytoplasm but is dispensible for targeting to the secretion loci. Mol Microbiol 16: 635-647.
13. Gietz, R.D., and Woods, R.A. (1994) High efficiency transformation in yeast. In Molecular Genetics of Yeast: Practical Approaches. Johnson, J.A. (ed). Oxford: Oxford University Press, pp. 121-134.
14. Gómez-Duarte, O.G., and Kaper, J.B. (1995) A plasmid-encoded regulatory region activates chromosomal eaeA expression in enteropathogenic Escherichia coli. Infect Immun 63: 1767-1776.
15. Hartland, E.L., Batchelor, M., Delahay, R.M., Hale, C., Matthews, S., Dougan, G., Knutton, S., Connerton, I., and Frankel, G. (1999) Binding of intimin from enteropathogenic Escherichia coli to Tir and to host cells. Mol Microbiol 32: 151-158.
16. Hueck, C.J. (1998) Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol Mol Biol Rev 62: 379-433.
17. Iriarte, M., and Cornelis, G. (1998) YopT, a new Yersinia Yop effector protein, affects the cytoskeleton of host cells. Mol Microbiol 29: 915-929.
18. Jarvis, K.G., and Kaper, J.B. (1996) Secretion of extracellular proteins by enterohemorrhagic Escherichia coli via a putative type III secretion system. Infect Immun 64: 4826-4829.
19. Jarvis, K.G., Girón, J.A., Jerse, A.E., McDaniel, T.K., Donnenberg, M.S., and Kaper, J.B. (1995) Enteropathogenic Escherichia coli contains a putative type III secretion system necessary for the export of proteins involved in attaching and effacing lesion formation. Proc Natl Acad Sci USA 92: 7996-8000.
20. Jerse, A.E., and Kaper, J.B. (1991) The eae gene of enteropathogenic Escherichia coli encodes a 94-kilodalton membrane protein, the expression of which is influenced by the EAF plasmid. Infect Immun 59: 4302-4309.
21. Jerse, A.E., Martin, W.C., Galen, J.E., and Kaper, J.B. (1990) An oligonucleotide probe for the detection of the EPEC adherence factor (EAF) of localized adherent enteropathogenic Escherichia coli. J Clin Microbiol 28: 2842-2844.
22. Kaper, J.B., Elliott, S.J., Sperandio, V., Perna, N.T., Mayhew, G.F., and Blattner, F.R. (1998) Attaching-and-effacing intestinal histopathology and the locus of enterocyte effacement. In Escherichia coli O157:H7 and other Shiga Toxin-Producing E. coli Strains. Kaper, J.B. and O'Brien, A.D. (eds). Washington DC: American Society for Microbiology Press, pp. 163-182.
23. Kenny, B. (1999) Phosphorylation of tyrosin 474 of the enteropathogenic Escherichia coli (EPEC) Tir receptor molecule is essential for actin nucleating activity and is preceded by additional host modifications. Mol Microbiol 31: 1229-1241.
24. Kenny, B., DeVinney, R., Stein, M., Reinscheid, D.J., Frey, E.A., and Finlay, B.B. (1997) Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells. Cell 91: 511-520.
25. Knutton, S., Baldwin, T., Williams, P.H., and McNeish, A.S. (1989) Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli. Infect Immun 57: 1290-1298.
26. Knutton, S., Rosenshine, I., Pallen, M.J., Nisan, I., Neves, B.C., Bain, C., Wolff, C., Dougan, G., and Frankel, G. (1998) A novel EspA-associated surface organelle of enteropathogenic Escherichia coli involved in protein translocation into epithelial cells. EMBO J 17: 2166-2176.
27. Kubori, T., Matsushima, Y., Nakamura, D., Uralil, J., Lara-Tejero, M., Sukhan, A., Galàn, J.E., and Aizawa, S.I. (1998) Supramolecular structure of the Salmonella typhimurium type III protein secretion system. Science 280: 602-605.
28. Kumar, A., Malloch, R.A., Fujita, N., Smillie, D.A., Ishihama, A., and Hayward, R.S. (1993) The minus 35-recognition region of Escherichia coli sigma 70 is inessential for initiation of transcription at an 'extended minus 10' promoter. J Mol Biol 232: 406-418.
29. Levine, M.M., Bergquist, E.J., Nalin, D.R., Waterman, D.H., Hornick, R.B., Young, C.R., Sotman, S., and Rowe, B. (1978) Escherichia coli strains that cause diarrhoea but do not produce heat-labile or heat-stable enterotoxins and are non-invasive. Lancet 1: 1119-1122.
30. McDaniel, T.K., and Kaper, J.B. (1997) A cloned pathogenicity island from enteropathogenic Escherichia coli confers the attaching and effacing phenotype on E. coli K-12. Mol Microbiol 23: 399-407.
31. Mellies, J.L., Elliott, S.J., Sperandio, V., Donnenberg, M.S., and Kaper, J.B. (1999) The Per regulon of enteropathogenic Escherichia coli: identification of a regulatory cascade and a novel transcriptional activator, the locus of enterocyte effacement (LEE)-encoded regulator (Ler). Mol Microbiol 33: 296-306.
32. Ménard, R., Sansonetti, P., Parsot, C., and Vasselon, T. (1994) Extracellular association and cytoplasmic partitioning of the IpaB and IpaC invasins of S flexneri. Cell 79: 515-525.
33. Neyt C., and Cornelis, G.R. (1999) Role of SycD, the chape-rone of the Yersinia Yop translocators YopB and YopD. Mol Microbiol 31: 143-156.
34. Noriega, F.R., Losonsky, G., Lauderbaugh, C., Liao, F.M., Vang, J.Y., and Levine, M.M (1996) Engineered ΔguaB-A ΔvirG Shigella flexneri 2a strain CVD 1205: construction, -aiety, immunogenicity, and potential efficacy as a muco_ai vaccine. Infect Immun 64: 3055-3061.
35. Penfeld, R.J., and Pemberton, J.M. (1992) An improved suicide vector for construction of chromosomal insertion mutations in bacteria. Gene 118: 145-146.
36. Perna, N.T., Mayhew, C.F., Pósfal, G., Elliott, S. Donnenberg, M.S., Kaper J B., and Blattner, F.R. (1998) Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect Immun 66: 3810-3817.
37. Roine, E., Wei, W., Yuan, J., Nurmiaho-Lassila, E.L., Kalkkiner, N. Romantschuk, M., and He, S.Y. (1997) Hrp pilus: an nro-dependent bacterial surface appendage produced by Pseudomonas svringae pv. tomato DC3000. Proc Natl Acad Sci USA 94: 3459-3464.
38. Rosenshine, I., Donnenberg, M.S., Kaper, J.B., and Finlay, B.B. (1992) Signal transduction between enteropathogenic Escherichia coli (EPEC) and epithelial cells: EPEC induces tyrosine phosphorylation of host cell proteins to initiate cytoskeletal rearrangement and bacterial uptake. EMBO 61: 3551-3560.
39. Sory, M.-P., and Cornelis, G.R. (1994) Translocation of a hybrid YopE-adenylate cyclase from Yersinia enterocolitica into HeLa a cells. Mol Microbiol 14: 583-594.
40. Sory, M.-P., Boland, A., Lambermont, I., and Cornelis, G.R (1995) Identification of the YopE and YopH domains required for secretion and internalization into the cytosol of macrophages, using the cyaA gene fusion approach. Proc Natl Aced Sci USA 92: 11998-12002.
41. Stainier, I., Iriarte, M., and Cornelis, G.R. (1997) YscM1 and YscM2, two Yersinia enterocolitica proteins causing downregulation of yop transcription. Mol Microbiol 26: 833-843.
42. Wainwright, L.A., and Kaper, J.B. (1998) EspB and EspD require a specific chaperone for proper secretion from enteropathogenic E coli. Mol Microbiol 27: 1247-1260.
43. Wattiau, P., and Cornelis, G.R. (1993a) Response to 'Yet another chaperone?'. Trends Microbiol 1: 162-163.
44. Wattiau, P., and Cornelis, G.R. (1993b) SycE, a chaperone-like protein of Yersinia enterocolitica involved in the secretion of YopE. Mol Microbiol 8: 123-131.
45. Wattiau, P., Woestyn, S., and Cornelis, G.R. (1996) Customizod secretion chaperones in pathogenic bacteria. Mol Microbiol 20: 255-262.
46. Woestyn, S., Sory, M.P., Boland, A., Lequenne, O., and Cornelis, G.R. (1996) The cytosolic SycE and SycH chaperones of Yersinia protect the region of YopE and YopH involved in translocation across eukaryotic cell membranes. Mol Microbiol 20: 1261-1271.
47. Yu, J., and Kaper, J.B. (1992) Cloning and characterization of the eae gene of enterohaemorrhagic Escherichia coli O157:H7. Mol Microbiol 6: 411-417.