EspE, a novel secreted protein of attaching and effacing bacteria, is directly translocated into infected host cells, where it appears as a tyrosine-phosphorylated 90 kDa protein

Molecular Microbiology

Volumn 28, Issue 3, 1998, Pages 463-474

  Deibel, Christina ^a   Krämer, Sylvia ^a   Chakraborty, Trinad ^a   Ebel, Frank ^a,b  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

^b UNIVERSITY HOSPITAL GIESSEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; BACTERIAL PROTEIN; MONOCLONAL ANTIBODY; SHIGA TOXIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM ADHERENCE; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; ESCHERICHIA COLI O157; HAFNIA ALVEI; HELA CELL; HOST CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN SECRETION; PROTEIN TRANSPORT; TEMPERATURE DEPENDENCE;

ACTINS; AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; BACTERIAL ADHESION; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI O157; ESCHERICHIA COLI PROTEINS; HEAT; HELA CELLS; HUMANS; IMMUNOASSAY; IMMUNOBLOTTING; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; PHOSPHOPROTEINS; PHOSPHORYLATION; PHOSPHOTYROSINE; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SIGNAL TRANSDUCTION;

EID: 0031960239     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1998.00798.x     Document Type: Article

References (28)

1. Abe, A., Kenny, B., Stein, M., and Finlay, B.B. (1997) Characterization of two virulence proteins secreted by rabbit enteropathogenic Escherichia coli, EspA and EspB, whose maximal expression is sensitive to host body temperature. Infect Immun 65: 3547-3555.
2. Albert, M.J., Faruque, S.M., Ansaruzzaman, M., et al. (1992) Sharing of virulence-associated properties at the phenotypic and genetic levels between enteropathogenic Escherichia coli and Hafnia alvei. J Med Microbiol 37: 310-314.
3. Bairoch, A. (1992) PROSITE: a dictionary of sites and patterns in proteins. Nucleic Acids Res 20: 2013-2018.
4. Brunder, W., Schmidt, H., and Karch, H. (1997) EspP, a novel extracellular serine protease of enterohemorrhagic Escherichia coli O157: H7 cleaves human coagulation factor V. Mol Microbiol 24: 767-778.
5. Djafari, S., Ebel, F., Deibel, C., Krämer, S., Hudel, M., and Chakraborty, T. (1997) Characterization of an exported protease from Shiga toxin-producing Escherichia coli. Mol Microbiol 25: 771-784.
6. Donnenberg, M.S., and Kaper, J.B. (1991) Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect Immun 59: 4310-4317.
7. Donnenberg, M.S., Yu, J., and Kaper, J.B. (1993) A second chromosomal gene necessary for intimate attachment of enteropathogenic Escherichia coli to epithelial cells. J Bactenol 175: 4670-4680.
8. Donnenberg, M.S., Kaper, J.B., and Finlay, B.B. (1997) Interactions between enteropathogenic Escherichia coli and host epithelial cells. Trends Microbiol 5: 109-114.
9. Ebel, F., Deibel, C., Kresse, A.U., Guzmán, C., and Chakraborty, T. (1996) Temperature- and medium-dependent secretion of proteins by Shiga toxin-producing Escherichia coli. Infect Immun 64: 4472-4479.
10. Foubister, V., Rosenshine, I., and Finlay, B.B. (1994) A diarrheal pathogen, enteropathogenic Escherichia coli (EPEC), triggers a flux of inositol phosphates in infected epithelial cells. J Exp Med 179: 993-998.
11. Griffin, P.M., Bell, B.P., Cieslak, P.R., et al. (1994) Large outbreak of Escherichia coli infections in the western United States: the big picture. In Recent Advances in Verocytotoxin-producing Escherichia coli Infections. Karmali, M.A., and Goglio, A.G. (eds). Amsterdam: Elsevier Science, pp. 7-12.
12. Harlow, E., and Lane, D. (1988) Antibodies - A laboratory manual. Cold Spring Harbor Laboratory: Cold Spring Harbor, New York.
13. Ismaili, A., Philpott, D.J., Dytoc, M.T., and Sherman, P.M. (1995) Signal transduction responses following adhesion of verotoxin-producing Escherichia coli. Infect Immun 63: 3316-3326.
14. Jarvis, K.G., Giron, J.A., Jerse, A.E., McDaniel, T.K., Donnenberg, M.S., and Kaper, J.B. (1995) Enteropathogenic Escherichia coli contains a putative type III secretion system necessary for the export of proteins involved in attaching and effacing lesion formation. Proc Natl Acad Sci USA 92: 7996-8000.
15. Jerse, A.E., Yu, J., Tall, B.D., and Kaper, J.B. (1990) A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Natl Acad Sci USA 59: 4302-4309.
16. Kenny, B., and Finlay, B.B. (1995) Protein secretion by enteropathogenic Escherichia coli is essential for transducing signals to epithelial cells. Proc Natl Acad Sci USA 92: 7991-7995.
17. Kenny, B., Lai, L.C., Finlay, B.B., and Donnenberg, M.S. (1996) EspA, a protein secreted by enteropathogenic Escherichia coli, is required to induce signals in epithelial cells. Mol Microbiol 20: 313-323.
18. Lai, L.C., Wainwright, L.A., Stone, K.D., and Donnenberg, M.S. (1997) A third secreted protein that is encoded by the enteropathogenic Escherichia coli pathogenicity island is required for transduction of signals and for attaching and effacing activities in host cells. J Bacteriol 65: 2211-2217.
19. Lee, C.A. (1997) Type III secretion systems: machines to deliver bacterial proteins into eukaryotic cells? Trends Microbiol 5: 148-156.
20. McDaniel, T.K., Jarvis, K.G., Donnenberg, M.S., and Kaper, J.B. (1995) A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc Natl Acad Sci USA 92: 1664-1668.
21. Moake, J.L. (1994) Haemolytic-uraemic syndrome: basic science. Lancet 343: 1042.
22. O'Brien, A.D., Lively, T.A., Chen, M.E., Rothman, S.W., and Formal, S.B. (1983) Escherichia coli O157: H7 strains associated with hemorrhagic colitis in the United States produce a Shigella dysenteriae 1 (SHIGA)-like cytotoxin. Lancet 1: 702.
23. Pawson, T., and Schlessinger, J. (1993) SH2 and SH3 domains. Curr Biol 3: 434-442.
24. Romer, L.H., Burridge, K., and Turner, C.E. (1992) Signaling between the extracellular matrix and the cytoskeleton: tyrosine phosphorylation and focal adhesion assembly. Cold Spring Harb Symp Quant Biol 57: 193-202.
25. Rosenshine, I., Donnenberg, M.S., Kaper, J.B., and Finlay, B.B. (1992) Signal transduction between enteropathogenic Escherichia Coli (EPEC) and epithelial cells: EPEC induces tyrosine phosphorylation of host cell proteins to initiate cytoskeletal rearrangements and bacterial uptake. EMBO J 11: 3551-3560.
26. Rosenshine, I., Ruschkowski, S., Stein, M., Reinscheid, D.J., Mills, S.D., and Finlay, B.B. (1996) A pathogenic bacterium triggers epithelial signals to form a functional bacterial receptor that mediates actin pseudopod formation. EMBO J 15: 2613-2624.
27. Sanger, M.J., Chang, R., Ashton, F., Kaper, J.B., and Sanger, J.W. (1996) Novel form of actin-based motility transports bacteria on the surfaces of infected cells. Cell Motil Cytoskeleton 34: 279-287.
28. Wieler, L.H., Tigges, M., Ebel, F., Schäferkordt, S., Djafari, S., Schlapp, T., Baljer, G., and Chakraborty, T. (1996) The enterohemolysin phenotype of Shiga-like toxin-producing Escherichia coli (SLTEC) is encoded by the EHEC-hemolysin gene. Vet Microbiol 52: 153-164.