Identification of the intimin-binding domain of Tir of enteropathogenic Escherichia coli

Cellular Microbiology

Volumn 1, Issue 1, 1999, Pages 7-17

  De Grado, Myriam ^a   Abe, Akio ^a,b   Gauthier, Annick ^a   Steele Mortimer, Olivia ^a   DeVinney, Rebekah ^a   Finlay, B Brett ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b KITASATO INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; ADHESIN; CARRIER PROTEIN; CELL SURFACE RECEPTOR; EAEA PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; OUTER MEMBRANE PROTEIN; RECOMBINANT PROTEIN; TIR PROTEIN, E COLI; TYROSINE;

ARTICLE; CHEMISTRY; COMPARATIVE STUDY; EPITHELIUM CELL; ESCHERICHIA COLI; FLUORESCENT ANTIBODY TECHNIQUE; GENE DELETION; GENETIC TRANSFECTION; GENETICS; HELA CELL; HUMAN; IMMUNOBLOTTING; METABOLISM; MICROBIOLOGY; MUTATION; PATHOGENICITY; PATHOLOGY; PHOSPHORYLATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; SACCHAROMYCES CEREVISIAE;

ACTINS; ADHESINS, BACTERIAL; BACTERIAL OUTER MEMBRANE PROTEINS; CARRIER PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EPITHELIAL CELLS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLUORESCENT ANTIBODY TECHNIQUE; GENE DELETION; HELA CELLS; HUMANS; IMMUNOBLOTTING; MUTATION; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, CELL SURFACE; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; TRANSFECTION; TYROSINE;

EID: 0033157102     PISSN: 14625814     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1462-5822.1999.00001.x     Document Type: Article

References (27)

1. DeVinney, R., Stein, M., Reinschied, D., Abe, A., Ruschkowski, S., and Finlay, B.B. (1999) Enterohemorrhagic Escherichia coli O157: H7 produces Tir which is translocated to the host cell membrane, but not tyrosine phosphorylated. Infect Immun 67: 2389-2398.
2. Donnenberg, M.S., and Kaper, J.B. (1991) Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect Immun 59: 4310-4317.
3. Donnenberg, M.S., Donohue-Rolfe, A., and Keusch, G.T. (1990) A comparison of HEp-2 cell invasion by enteropathogenic and enteroinvasive Escherichia coli. FEMS Microbiol Lett 57: 83-86.
4. Donnenberg, M.S., Tacket, C.O., James, S.P., Losonsky, G., Nataro, J.P., Wasserman, S.S., et al. (1993a) Role of the eaeA gene in experimental enteropathogenic Escherichia coli infection [see comments]. J Clin Invest 92: 1412-1417.
5. Donnenberg, M.S., Yu, J., and Kaper, J.B. (1993b) A second chromosomal gene necessary for intimate attachment of enteropathogenic Escherichia coli to epithelial cells. J Bacteriol 175: 4670-4680.
6. Elliott, S.J., Wainwright, L.A., McDaniel, T.K., Jarvis, K.G., Deng, Y.K., Lai, L.C., et al. (1998) The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69. Mol Microbiol 28: 1-4.
7. Finlay, B.B., Ruschkowski, S., and Dedhar, S. (1991) Cytoskeletal rearrangements accompanying salmonella entry into epithelial cells. J Cell Sci 99: 283-296.
8. Foubister, V., Rosenshine, I., and Finlay, B.B. (1994) A diarrheal pathogen, enteropathogenic Escherichia coli (EPEC), triggers a flux of inositol phosphates in infected epithelial cells. J Exp Med 179: 993-998.
9. Frankel, G., Candy, D.C., Everest, P., and Dougan, G. (1994) Characterization of the C-terminal domains of intimin-like proteins of enteropathogenic and enterohemorrhagic Escherichia coli, Citrobacter freundii, and Hafnia alvel. Infect Immun 62: 1835-1842.
10. Frankel, G., Lider, O., Hershkoviz, R., Mould, A.P., Kachalsky, S.G., Candy, D.C.A., et al. (1996) The cell-binding domain of intimin from enteropathogenic Escherichia coli binds to betal integrins. J Biol Chem 271: 20359-20364.
11. Frankel, G., Phillips, A.D., Rosenshine, G.D., Kaper, J.B., and Knutton, S. (1998) Enteropathogenic and enterohaemorrhagic Escherichia coli: more subversive elements. Mol Microbiol 30: 911-921.
12. Giron, J.A., Ho, A.S., and Schoolnik, G.K. (1991) An inducible bundle-forming pilus of enteropathogenic Escherichia coli. Science 254: 710-713.
13. Goosney, D.L., de Grado, M., and Finlay, B.B. (1999) Putting E. coli on a pedestal: a unique system to study signal transduction and the actin cytoskeleton. Trends Cell Biol 9: 11-14.
14. Hueck, C.J. (1998) Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol Mol Biol Rev 62: 379-433.
15. Jerse, A.E., and Kaper, J.B. (1991) The eae gene of enteropathogenic Escherichia coli encodes a 94- kilodalton membrane protein, the expression of which is influenced by the EAF plasmid. Infect Immun 59: 4302-4309.
16. Kaper, J.B. (1998) EPEC delivers the goods. Trends Microbiol 6: 169-172.
17. Kenny, B., and Finlay, B.B. (1997) Intimin-dependent binding of enteropathogenic Escherichia coli to host cells triggers novel signaling events, including tyrosine phosphorylation of phospholipase C-gamma1. Infect Immun 65: 2528-2536.
18. Kenny, B., Lai, L.C., Finlay, B.B., and Donnenberg, M.S. (1996) EspA, a protein secreted by enteropathogenic Escherichia coli, is required to induce signals in epithelial cells. Mol Microbiol 20: 313-323.
19. Kenny, B., DeVinney, R., Stein, M., Reinscheid, D.J., Frey, E.A., and Finlay, B.B. (1997a) Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells. Cell 91: 511-520.
20. Kenny, B., Abe, A., Stein, M., and Finlay, B.B. (1997b) Enteropathogenic Escherichia coli protein secretion is induced in response to conditions similar to those in the gastrointestinal tract. Infect Immun 65: 2606-2612.
21. Lai, L.C., Wainwright, L.A., Stone, K.D., and Donnenberg, M.S. (1997) A third secreted protein that is encoded by the enteropathogenic Escherichia coli pathogenicity island is required for transduction of signals and for attaching and effacing activities in host cells. Infect Immun 65: 2211-2217.
22. McDaniel, T.K., Jarvis, K.G., Donnenberg, M.S., and Kaper, J.B. (1995) A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc Natl Acad Sci USA 92: 1664-1668.
23. Rosenshine, I., Donnenberg, M.S., Kaper, J.B., and Finlay, B.B. (1992) Signal transduction between enteropathogenic Escherichia coli (EPEC) and epithelial cells: EPEC induces tyrosine phosphorylation of host cell proteins to initiate cytoskeletal rearrangement and bacterial uptake. EMBO J 11: 3551-3560.
24. Rosenshine, I., Ruschkowski, S., Stein, M., Reinscheid, D.J., Mills, S.D., and Finlay, B.B. (1996a) A pathogenic bacterium triggers epithelial signals to form a functional bacterial receptor that mediates actin pseudopod formation. EMBO J 15: 2613-2624.
25. Rosenshine, I., Ruschkowski, S., and Finlay, B.B. (1996b) Expression of attaching/effacing activity by enteropathogenic Escherichia coli depends on growth phase, temperature, and protein synthesis upon contact with epithelial cells. Infect Immun 64: 966-973.
26. Williams, J.M., Chen, G.C., Zhu, L., and Rest, R.F. (1998) Using the yeast two-hybrid system to identify human epithelial cell proteins that bind gonococcal Opa proteins: intracellular gonococci bind pyruvate kinase via their Opa proteins and require host pyruvate for growth. Mol Microbiol 27: 171-186.
27. Yu, J., and Kaper, J.B. (1992) Cloning and characterization of the eae gene of enterohaemorrhagic Escherlchia coli O157:H7. Mol Microbiol 6: 411-417.