Molecular biology techniques in parasite ecology

International Journal for Parasitology

Volumn 32, Issue 5, 2002, Pages 551-562

  Monis, Paul T ^a   Andrews, Ross H ^b   Saint, Christopher P ^a  

^a AUSTRALIAN WATER QUALITY CENTRE   (Australia)

^b UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

Ecology; Flow cytometry; In situ hybridisation; Molecular biology; Parasites; Polymerase chain reaction

Indexed keywords

ISOENZYME;

FLOW CYTOMETRY; MOLECULAR ECOLOGY; PARASITOLOGY; POLYMERASE CHAIN REACTION;

ANALYTIC METHOD; BACTERIUM; BIOGEOGRAPHY; CELL LABELING; CONFERENCE PAPER; DNA FINGERPRINTING; ECOLOGY; ENDOSYMBIONT; ENZYME ELECTROPHORESIS; FLOW CYTOMETRY; HYBRIDIZATION; IN SITU HYBRIDIZATION; LIGASE CHAIN REACTION; MICROFLORA; MOLECULAR BIOLOGY; NONHUMAN; NUCLEIC ACID AMPLIFICATION; NUCLEOTIDE SEQUENCE; PARASITOLOGY; PHYLOGENY; POLYMERASE CHAIN REACTION; POPULATION GENETIC PARAMETERS; PROTOZOON; TECHNIQUE; TICK; VIRUS;

ANIMALS; ECOSYSTEM; HOST-PARASITE RELATIONS; HUMANS; MOLECULAR BIOLOGY; PARASITES;

ACARI; BACTERIA (MICROORGANISMS); PROTOZOA;

EID: 0036211119     PISSN: 00207519     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0020-7519(01)00352-6     Document Type: Conference Paper

References (143)

1. Modes of transmission and evolution of life histories in zooparasitic nematodes
2. Microsatellite analysis of Toxoplasma gondii shows considerable polymorphism structured into two main clonal groups
3. Coevolution of hosts and parasites
4. Common problems in the application of analytical techniques to parasitology
5. Multilocus enzyme electrophoresis: A valuable technique for providing answers to problems in parasite systematics
6. Genetic characteristics of Leishmania isolates at 37 enzyme loci
7. Giardia intestinalis: Electrophoretic evidence for a species complex
8. One-tube real-time isothermal amplification assay to identify and distinguish human immunodeficiency virus type 1 subtypes a, b, and c and circulating recombinant forms ae and ag
9. Random amplified polymorphic DNA (RAPD) and parsimony methods
10. Random amplified polymorphic DNA fingerprints on the eight taxa of Trichinella and their comparison with allozyme analysis
11. Genetic disease detection and DNA amplification using cloned thermostable ligase
12. Molecular phylogeny of Schistosoma species supports traditional groupings within the genus
13. The molecular epidemiology of Giardia lamblia: A sequence-based approach
14. PCR bias in ecological analysis: A case study for quantitative Taq nuclease assays in analyses of microbial communities
15. Phylogenetic relationships among tick subfamilies (Ixodida; Ixodidae: Argasidae) based on the 18S nuclear rDNA gene
16. DNA fingerprinting of Cryptosporidium parvum isolates using amplified fragment length polymorphism (AFLP)
17. Characterization of carbohydrate binding proteins in Trypanosoma cruzi
18. Detection of malaria liver-stages in mice infected through the bite of a single Anopheles mosquito using a highly sensitive real-time PCR
19. Clonal and geographic distribution of a surface antigen of Tritrichomonas foetus
20. Analysis of adhesion and cytotoxicity of Tritrichomonas foetus to mammalian cells by use of monoclonal antibodies
21. On the fittedness of organisms and the ascendency of ecosystems - Toward a hierarchical model of network development
22. Parasitology year 2000
23. Large sequence variation at two microsatellite loci among zoonotic (genotype C) isolates of Cryptosporidium parvum
24. Homogeneous genotyping assays for single nucleotide polymorphisms with fluorescence resonance energy transfer detection
25. A homogeneous, ligase-mediated DNA diagnostic test
26. Application of digital image analysis and flow cytometry to enumerate marine viruses stained with SYBR gold
27. Nucleic acid sequence-based amplification
28. Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR
29. In situ detection of high levels of horizontal plasmid transfer in marine bacterial communities
30. Populations, genetic variation, and the delimitation of phylogenetic species
31. Rapid method for fluorescent in situ ribosomal RNA labelling of Cryptosporidium parvum
32. Parasite genome initiatives
33. Methods for visualizing RNA processing and transport pathways in living cells
34. Regulation and stability of a free-living host-parasite system: Trichostrongylus tenius in red grouse. II. Population models
35. Phylogenetic analysis of Sarcocystis spp. of mammals and reptiles supports the coevolution of Sarcocystis spp. with their final hosts
36. Oligonucleotide probes for specific detection of Giardia lamblia cysts by fluorescent in situ hybridization
37. Molecular epidemiological survey of rotaviruses in sewage by reverse transcriptase seminested PCR and restriction fragment length polymorphism assay
38. Pathogen analysis and genetic predisposition testing using microelectronic arrays and isothermal amplification
39. Simultaneous detection of influenza viruses A and B using real-time quantitative PCR
40. Extensive polymorphism in Cryptosporidium parvum identified by multilocus microsatellite analysis
41. Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community
42. Long, polymorphic microsatellites in simple organisms
43. Parasites as probes for biodiversity
44. What's in that band?
45. Identification of parasitic nematodes by PCR-SSCP of ITS-2 rDNA
46. Genotyping Cryptosporidium parvum by single-strand conformation polymorphism analysis of ribosomal and heat shock gene regions
47. Community structure and activity dynamics of nitrifying bacteria in a phosphate-removing biofilm
48. Comparison of the urine-based ligase chain reaction test to culture for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in pediatric sexual abuse victims
49. Bacterioplankton compositions of lakes and oceans: A first comparison based on fluorescence in situ hybridization
50. Microtubule-independent motility and nuclear targeting of adenoviruses with fluorescently labeled genomes
51. Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication
52. Toxoplasma gondii virulence markers identified by random amplified polymorphic DNA polymerase chain reaction
53. Real time quantitative PCR
54. Highly sensitive detection of gene expression of an intronless gene: Amplification of mRNA, but not genomic DNA by nucleic acid sequence based amplification (NASBA)
55. Giardia muris trophozoite antigenic targets for mouse intestinal IgA antibody
56. Use of mobile genetic elements as tools for molecular epidemiology
57. Kinetic PCR analysis: Real-time monitoring of DNA amplification reactions
58. Forks and combs and DNA: The synthesis of branched oligodeoxyribonucleotides
59. Genetic variation within the ticks Ixodes holocyclus and Ixodes cornuatus from South-eastern Australia
60. Development of a real-time PCR assay for detection of Toxoplasma gondii in pig and mouse tissues
61. Characterization of the subunits of Q-beta replicase
62. Detection of Cryptosporidium oocysts in water: Comparison of the conventional microscopic immunofluorescence technique with PCR and Taqman™ PCR
63. Coevolutionary dynamics of sex in a metapopulation - Escaping the Red Queen
64. Characterization of human AFLP systems apolipoprotein B, phenylalanine hydroxylase, and D1S80
65. Genetic differentiation of Anopheles gambiae populations from East and West Africa: Comparison of microsatellite and allozyme loci
66. Molecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA
67. Direct detection of Mycobacterium tuberculosis complex in clinical samples from patients in Norway by ligase chain reaction
68. Allelic discrimination using fluorogenic probes and the 5′ nuclease assay
69. Rapid identification of Campylobacter spp. by melting peak analysis of biprobes in real-time PCR
70. Comparative study of conventional and novel strategies for the detection of hepatitis C virus RNA in serum: Amplicor, branched-DNA, NASBA and in-house PCR
71. Cestode systematics: Any progress?
72. Isoenzyme electrophoresis of 30 isolates of Giardia from humans and felines
73. Detection and identification of indicator bacteria using rRNA targeted probes
74. Autocatalytic replication of a recombinant RNA
75. Parasitism: A cryptic determinant of animal community structure
76. In situ gene expression in mixed-culture biofilms: Evidence of metabolic interactions between community members
77. The importance of systematics in parasitological research
78. Molecular epidemiology - Assumptions and limitations of commonly applied methods
79. Development of a nested-PCR assay for the detection of Cryptosporidium parvum in finished water
80. Molecular genetic analysis of Giardia intestinalis isolates at the glutamate dehydrogenase locus
81. Molecular systematics of the parasitic protozoan Giardia intestinalis
82. Best viral elution method available for quantification of enteroviruses in sludge by both cell culture and reverse transcription-PCR
83. Phylogenetic relationships among isolates of Cryptosporidium: Evidence for several new species
84. Molecular and phylogenetic characterisation of Cryptosporidium from birds
85. Leishmania and Sauroleishmania: The use of random amplified polymorphic DNA for the identification of parasites from vertebrates and invertebrates
86. Specific enzymatic amplification of DNA in vitro: The polymerase chain reaction
87. Systematics of mosquito disease vectors (Diptera, Culicidae): Impact of molecular biology and cladistic analysis
88. Proteins: Isozyme electrophoresis
89. The application of molecular markers in the study of diversity in acarology: A review
90. Quantitative reverse transcription strand displacement amplification: Quantitation of nucleic acids using an isothermal amplification technique
91. Evidence for host-specific clades of tetraphyllidean tapeworms (Platyhelminthes: Eucestoda) revealed by analysis of 18S ssrDNA
92. Differentiation among three species of bovine Thelazia (Nematoda: Thelaziidae) by polymerase chain reaction-restriction fragment length polymorphism of the first internal transcribed spacer ITS-1 (rDNA)
93. Amplified fragment length polymorphism analysis of genetic diversity of Haemonchus contortus during selection for drug resistance
94. Evaluation of the Gen-Probe Chlamydia trachomatis transcription-mediated amplification assay with urine specimens from women
95. Microbial gene transfer: An ecological perspective
96. New molecular markers for phlebotomine sand flies
97. Molecular beacon sequence analysis for detecting drug resistance in Mycobacterium tuberculosis
98. Rapid differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto by LightCycler fluorescence melting curve analysis of a PCR product of the recA gene
99. Flow cytometric detection of specific genes in genetically modified bacteria using in situ polymerase chain reaction
100. General concepts on the evolutionary biology of parasites
101. Diversity of feline Chlamydia psittaci revealed by random amplification of polymorphic DNA
102. A preliminary study of the population genetics of Aedes aegypti (Diptera: Culicidae) from Mexico using microsatellite and AFLP markers
103. Linked linear amplification: A new method for the amplification of DNA
104. Molecular epidemiologic evaluation of transmissibility and virulence of Mycobacterium tuberculosis
105. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
106. Quantitation of HIV-1 by real-time PCR with a unique fluorogenic probe
107. Amplified-fragment length polymorphism analysis: The state of an art
108. Polymerase chain reaction for diagnosis and identification of distinct variants of Crimean-Congo hemorrhagic fever virus in the United Arab Emirates
109. Q-beta replicase-amplified assay for detection of Mycobacterium tuberculosis directly from clinical specimens
110. Microbial analysis at the single-cell level: Tasks and techniques
111. The evolutionary genetics of plant-pathogen systems. Understanding the coevolution of hosts and parasites is key to understanding their ecology
112. An RNA transcription-based amplification technique (NASBA) for the detection of viable Salmonella enterica
113. Characterisation of random amplified polymorphic DNA (RAPD) products from Xanthomonas campestris and some comments on the use of RAPD products in phylogenetic analysis
114. Real time detection of DNA.RNA hybridization in living cells
115. Detection of M. tuberculosis DNA using thermophilic strand displacement amplification
116. Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences
117. The phylogenetic status of Sarcobium lyticum, an obligate intracellular bacterial parasite of small amoebae
118. Detection of rRNA from four respiratory pathogens using an automated Q beta replicase assay
119. Use of polymerase chain reaction-based single strand conformational polymorphism and denaturing gradient gel electrophoresis methods for detection of sequence variation of ribosomal DNA of Trypanosoma cruzi
120. Phylogeny Inference
121. MGE-PCR: A novel approach to the analysis of Toxoplasma gondii strain differentiation using mobile genetic elements
122. Mode of action and application of Scorpion primers to mutation detection
123. Extremely sensitive, background-free gene detection using binary probes and beta replicase
124. Detection of HIV-1 RNA in plasma and serum samples using the NASBA amplification system compared to RNA-PCR
125. The use of a ribosomal RNA targeted oligonucleotide probe for fluorescent labelling of viable Cryptosporidium parvum oocysts
126. Multiplex detection of four pathogenic retroviruses using molecular beacons
127. Determination of complement-mediated killing of bacteria by viability staining and bioluminescence
128. Current and future applications of flow cytometry in aquatic microbiology
129. AFLP: A new technique for DNA fingerprinting
130. Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system
131. Flow sorting of microorganisms for molecular analysis
132. AFLP fingerprinting for assessing intraspecific variation and genome mapping in mites
133. Potential selective pressures by parasitoids on a plant-herbivore interaction
134. Antimicrobial resistance and bacterial identification utilizing a microelectronic chip array
135. Beta-tubulin mRNA as a marker of Cryptosporidium parvum oocyst viability
136. Discrimination of Listeria monocytogenes from other Listeria species by ligase chain reaction
137. Reconstructing phylogenies from allozyme data: Comparing method performance with congruence
138. Continuous fluorescence monitoring of rapid cycle DNA amplification
139. Real-time homogeneous assay of rapid cycle polymerase chain reaction product for identification of Leptonema illini
140. Identification of species and sources of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool
141. Molecular evolution and phylogeny of the human AIDS viruses LAV, HTLV-III, and ARV
142. HLA-DR generic typing by AFLP