Microbial analysis at the single-cell level: Tasks and techniques

Journal of Microbiological Methods

Volumn 42, Issue 1, 2000, Pages 3-16

  Shapiro, Howard M ^a  

^a Howard M Shapiro   (United States)

Author keywords

Bacteria; Flow cytometry; Fluorescent dyes; Membrane potential

Indexed keywords

FLUORESCENT DYE;

ANTIGEN SPECIFICITY; CELL ULTRASTRUCTURE; CELL VIABILITY; DIAGNOSTIC PROCEDURE; ENZYME ACTIVITY; MEMBRANE POTENTIAL; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MICROFLUOROMETRY; NONHUMAN; PRIORITY JOURNAL; REVIEW; SPECIES DIFFERENCE;

EID: 0033800421     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(00)00167-6     Document Type: Article

References (48)

1. Allman R., Hann A.C., Phillips A.P., Martin K.L., Lloyd D. Growth of Azotobacter vinelandii with correlation of Coulter cell size, flow cytometric parameters, and ultrastructure. Cytometry. 11:1990;822-831.
2. Asbury C.L., Uy J.L. van den Engh, G., 2000. The polarization of scatter and fluorescence signals in flow cytometry. Cytometry. 40:2000;88-101.
3. Chalfie M., Tu Y., Euskirchen G., Ward W.W., Prasher D.C. Green fluorescent protein as a marker for gene expression. Science. 263:1994;802-805.
4. Chou H.-P., Spence C., Scherer A., Quake S. A microfabricated device for sizing and sorting DNA molecules. Proc. Natl. Acad. Sci. USA. 96:1999;11-13.
5. Cubitt A.B., Heim R., Adams S.R., Boyd A.E., Gross L.A., Tsien R.Y. Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci. 20:1995;448-455.
6. Darzynkiewicz Z., Bedner E., Li X., Gorczyca W., Melamed M.R. Laser-scanning cytometry: a new instrumentation with many applications. Exp. Cell Res. 249:1999;1-12.
7. Davey H.M., Kell D.B. Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses. Microbiol. Rev. 60:1996;641-696.
8. De Blois R.W., Mayyasi S.A., Schidlovsky G., Wesley R., Wolff J.S. Virus counting and analysis by the resistive pulse (Coulter counter) technique. Proc. Am. Assoc. Cancer Res. 15:1974;104.
9. De Wulf, P., Brambilla, L., Vanoni, M., Porro, D., Alberghina, L., 2000. Real-time flow cytometric quantification of GFP expression and Gfp-fluorescence generation in Saccharomyces cerevisiae. J. Microbiol. Methods (this issue).
10. Denk W., Svoboda K. Photon upmanship: why multiphoton imaging is more than a gimmick. Neuron. 18:1997;351-357.
11. Dietz L.J., Dubrow R.S., Manian B.S., Sizto N.L. Volumetric capillary cytometry: a new method for absolute cell enumeration. Cytometry. 23:1996;177-186.
12. Dubelaar G.B.J., Visser J.W.M., Donze M. Anomalous behaviour of forward and perpendicular light scattering of a cyanobacterium owing to gas vacuoles. Cytometry. 8:1987;405-412.
13. Fu A.Y., Spence C., Scherer A., Arnold F.A., Quake S.R. A microfabricated fluorescence-activated cell sorter. Nat. Biotechnol. 17:1999;1109-1111.
14. Fulton R.J., McDade R.L., Smith P.L., Kienker J.J., Kettman J.R. Advanced multiplexed analysis with the Flow-Metrix™ system. Clin. Chem. 43:1997;1749-1756.
15. Gitin M., Ginouves P., Schulze M., Seelert W., Rosperich J., Pfaff J., Spinelli L. A compact solid-state 488 nm laser for cell analysis applications. Cytometry. (Suppl. 10):2000;71.
16. Gordon, C.L., Archer, D.B., Jeenes, D.J., Doonan, J.H., Wells, B., Trinci, A.P.J., Robson, G.D., 2000. A glucoamylase:GFP gene fusion to study protein secretion by individual hyphae of Aspergillus niger. J. Microbiol. Methods (this issue).
17. Gucker F.T. Jr., O'Konski C.T., Pickard H.B., Pitts J.N. Jr. A photoelectronic counter for colloidal particles. J. Am. Chem. Soc. 69:1947;2422-2431.
18. Hara S., Terauchi K., Koike I. Abundance of viruses in marine waters: assessment by epifluorescence and transmission electron microscopy. Appl. Environ. Microbiol. 57:1991;2731-2734.
19. Haugland R.P. Handbook of Fluorescent Probes and Research Chemicals. 6th Edition :1996;Molecular Probes, Eugene, OR.
20. Hennes K.P., Suttle C.A. Direct counts of viruses in natural waters and laboratory cultures by epifluorescence microscopy. Limnol. Oceanogr. 40:1995;1050-1055.
21. Hercher M., Mueller W., Shapiro H.M. Detection and discrimination of individual viruses by flow cytometry. J. Histochem. Cytochem. 27:1979;350-352.
22. Kamentsky L.A., Burger D.E., Gershman R.J., Kamentsky L.D., Luther E. Slide-based laser scanning cytometry. Acta Cytol. 41:1997;123-143.
23. Katsuragi, T., Tanaka, S., Nagahiro, S., Tani, Y., 2000. Gel microdroplet technique leaving microorganisms alive for sorting by flow cytometry. J. Microbiol. Methods (this issue).
24. Kubitschek H.E. Electronic counting and sizing of bacteria. Nature. 182:1958;234-235.
25. Lenkei R., Mandy F., Marti G., Vogt R. (eds). Quantitative fluorescence cytometry: an emerging consensus. Cytometry. 33:1998;93-287.
26. Marie D., Brussaard C.P.D., Thyrhaug R., Bratbak G., Vaulot D. Enumeration of marine viruses in culture and natural samples by flow cytometry. Appl. Environ. Microbiol. 65:1999;45-52.
27. Masters B.R., So P.T., Kim K.H., Buehler C., Gratton E. Multiphoton excitation microscopy, confocal microscopy, and spectroscopy of living cells and tissues; functional metabolic imaging of human skin in vivo. Methods Enzymol. 307:1999;513-536.
28. Mujumdar R.B., Ernst L.A., Mujumdar S.R., Lewis C.J., Waggoner A.S. Cyanine dye labeling reagents: sulfoindocyanine succinimidyl esters. Bioconjug. Chem. 4:1993;105-111.
29. Natarajan, A., Srienc, F., 2000. Glucose uptake rates of single E. coli cells grown in glucose-limited chemostat culture. J. Microbiol. Methods (this issue).
30. Nebe-von-Caron, G., Stephens, P.J., Hewitt, C.J., Powell, J.R., Badley, R.A., 2000. Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting. J. Microbiol. Methods (this issue).
31. Novo D., Perlmutter N.G., Hunt R.H., Shapiro H.M. Accurate flow cytometric membrane potential measurement in bacteria using diethyloxacarbocyanine and a ratiometric technique. Cytometry. 35:1999;55-63.
32. Novo D., Perlmutter N.G., Hunt R.H., Shapiro H.M. Multiparameter flow cytometric analysis of antibiotic effects on membrane potential, membrane permeability, and bacterial counts of Staphylococcus aureus and Micrococcus luteus. Antimicrob. Agents Chemother. 44:2000;827-834.
33. Oi V.T., Glazer A.N., Stryer L. Fluorescent phycobiliprotein conjugates for analyses of cells and molecules. J. Cell Biol. 93:1982;981-986.
34. Orsini, F., Ami, D., Villa, A.M., Sala, G., Bellotti, M.G., Doglia, S.M., 2000. FT-IR microspectroscopy for microbiological studies. J. Microbiol. Methods (this issue).
35. Porro, D., Venturini, M., Brambilla, L., Alberghina, L., Vanoni, M., 2000. Relating growth dynamics and glucoamylase excretion of individual Saccharomyces cerevisiae cells. J. Microbiol. Methods (this issue).
36. Porter, J., Pickup, R.W., 2000. Nucleic acid-based fluorescent probes in microbial ecology: application of flow cytometry J. Microbiol. Methods (this issue).
37. Robertson B.R., Button D.K., Koch A.L. Determination of the biomasses of small bacteria at low concentrations in a mixture of species with forward light scatter measurements by flow cytometry. Appl. Environ. Microbiol. 64:1998;3900-3909.
38. Schuster, K.C., Urlaub, E., Gapes, J.R., 2000. Single-cell analysis of bacteria by Raman microscopy: spectral information on the chemical composition of cells and on the heterogeneity in a culture. J. Microbiol. Methods (this issue).
39. Shapiro H.M. Practical Flow Cytometry. 3rd Edition :1995;412-425 Wiley-Liss, New York.
40. Shapiro H. The violet laser diode as a light source for cytometry. Cytometry. (Suppl. 10):2000;26.
41. Shapiro H.M., Perlmutter N.G., Stein P.G. A flow cytometer designed for fluorescence calibration. Cytometry. 33:1998;280-287.
42. Steen H.B., Boye E. Escherichia coli growth studied by dual-parameter flow cytophotometry. J. Bacteriol. 145:1980;1091-1094.
43. Steen, H.B., 2000. Flow cytometry of bacteria: glimpses from the past with a view to the future. J. Microbiol. Methods (this issue).
44. Szöllosi J., Damjanovich S., Mátyus L. Application of fluorescence resonance energy transfer in the clinical laboratory: routine and research. Cytometry (Comm. Clin. Cytometry). 34:1998;159-179.
45. Trask B.J., van den Engh G.J., Elgershuizen J.H.B.W. Analysis of phytoplankton by flow cytometry. Cytometry. 2:1982;258-264.
46. van den Engh G.J. Farmer, C., 1992. Photo-bleaching and photon saturation in flow cytometry. Cytometry. 13:1992;669-677.
47. Walberg M., Gaustad P., Steen H.B. Uptake kinetics of nucleic acid targeting dyes in S. aureus, E. faecalis and B. cereus: a flow cytometric study. J. Microbiol. Methods. 35:1998;167-176.
48. Weaver J.C., Seissler P.E., Threefoot S.A., Lorenz J.W., Huie T., Rodrigues R., Klibanov A.M. Microbiological measurements by immobilization of cells within small volume elements. Ann. New York Acad. Sci. 434:1984;363-372.