Development of a real-time PCR assay for detection of Toxoplasma gondii in pig and mouse tissues

Journal of Clinical Microbiology

Volumn 39, Issue 6, 2001, Pages 2065-2071

  Jauregui, L H ^a   Higgins, J ^a   Zarlenga, D ^a   Dubey, J P ^a   Lunney, J K ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL TISSUE; ARTICLE; MOUSE; NONHUMAN; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ASSAY; SWINE; TOXOPLASMA GONDII;

ANIMALS; DNA, PROTOZOAN; MICE; MUSCLES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SWINE; SWINE DISEASES; SWINE, MINIATURE; TAQ POLYMERASE; TOXOPLASMA; TOXOPLASMOSIS, ANIMAL;

ANIMALIA; PROTOZOA; SUS SCROFA; TOXOPLASMA GONDII;

EID: 0035013691     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.39.6.2065-2071.2001     Document Type: Article

References (31)

1. An eimeriid origin of isosporoid coccidia with Stieda bodies as shown by phylogenetic analysis of small subunit ribosomal RNA gene sequences
2. Real-time PCR for diagnosis and follow-up of Toxoplasma reactivation after allogeneic stem cell transplantation using fluorescence resonance energy transfer hybridization probes
3. Direct agglutination test for diagnosis of Toxoplasma infection: Method for increasing sensitivity and specificity
4. Toxoplasmosis
5. Oocyst shedding by cats fed isolated bradyzoites and comparison of infectivity of bradyzoites of the VEG strain Toxoplasma gondii to cats and mice
6. Infectivity of low numbers of Toxoplasma gondii oocysts to pigs
7. Distribution of Toxoplasma gondii tissue cysts in commercial cuts of pork
8. Detection of Toxoplasma gondii by PCR and tissue culture in cerebrospinal fluid and blood of human immunodeficiency virus-seropositive patients
9. Comparison of the large subunit ribosomal DNA of Neospora and Toxoplasma and development of a new genetic marker for their differentiation based on the D2 domain
10. Detection of Toxoplasma gondii in tissues of sheep orally challenged with different doses of oocysts
11. Detection of T. gondii in tissues of sheep and cattle following oral infection
12. The cost-effectiveness of preventing AIDS-related opportunistic infections
13. Prevalence of Toxoplasma gondii infection in domestic pigs in the New England states
14. Detection of parasites in food
15. Real time quantitative PCR
16. Identification of a 200-300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR
17. Toxoplasma gondii comprises three clonal lineages: Correlation of parasite genotype with human disease
18. A survey of meat samples from swine, cattle, and sheep for the presence of encysted Toxoplasma
19. Comparison of cell culture, mouse inoculation and PCR for detection of Toxoplasma gondii: Effects of storage conditions on sensitivity
20. Diagnosis of congenital Toxoplasma gondii infection by polymerase chain reaction (PCR) on amniotic fluid samples
21. Discrimination of Neospora caninum from Toxoplasma gondii and other apicomplexan parasites by hybrization and PCR
22. Measurement of the efficacy of vaccines and antimicrobial therapy against infection with Toxoplasma gondii
23. Cost-benefit analysis of screening for toxoplasmosis during pregnancy
24. Polymerase chain reaction for the detection of Toxoplasma gondii in aqueous humor of cats
25. Kinetics in parasite abundance in susceptible and resistant mice infected with an avirulent strain of Toxoplasma gondii by using quantitative competitive PCR
26. Food-related illness and death in the United States
27. Diagnosis of Toxoplasma abortion in ewes by polymerase chain reaction
28. Detection of Toxoplasma gondii in feline and canine biological samples by use of the polymerase chain reaction
29. Detection of Toxoplasma gondii in cured meats
30. Risk factors for transmission of Toxoplasma gondii on swine farms in Illinois