Xis protein binding to the left arm stimulates excision of conjugative transposon Tn916

Journal of Bacteriology

Volumn 184, Issue 8, 2002, Pages 2088-2099

  Connolly, Kevin M ^a   Iwahara, Mizuho ^a   Clubb, Robert T ^a  

^a Molecular Biology Institute 90095 1570   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CELL PROTEIN; INTEGRASE; UNCLASSIFIED DRUG; XIS PROTEIN;

ARTICLE; BACTERIOPHAGE LAMBDA; BINDING SITE; CHROMOSOME PAIRING; DNA STRAND BREAKAGE; ESCHERICHIA COLI; GENETIC RECOMBINATION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DNA INTERACTION; TRANSPOSON;

BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; CONJUGATION, GENETIC; DNA NUCLEOTIDYLTRANSFERASES; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; INTEGRATION HOST FACTORS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; VIRAL PROTEINS;

BACTERIA (MICROORGANISMS); BACTERIOPHAGE LAMBDA; ESCHERICHIA COLI; INSERTION SEQUENCES; NEGIBACTERIA; PROKARYOTA; TRANSPOSONS;

EID: 0036202271     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.8.2088-2099.2002     Document Type: Article

References (64)

1. Purification of the bacteriophage lambda xis gene product required for lambda excisive recombination
2. The Bacillus subtilis histone-like protein Hbsu is required for DNA resolution and DNA inversion mediated by the beta recombinase of plasmid pSM19035
3. The integrase family of site specific recombinases: Regional similarities and global diversity
4. Tn5253, the pneumococcal omega (cat tet) BM6001 element, is a composite structure of two conjugative transposons, Tn5251 and Tn5252
5. Conjugative transposition of Tn916: The transposon int gene is required only in the donor
6. Control of directionality in lambda site specific recombination
7. Determinants of directionality in lambda site-specific recombination
8. Excision and insertion of the conjugative transposon Tn916 involves a novel recombination mechanism
9. Genetic linkage and cotransfer of a novel, vanB-containing transposon (Tn5382) and a low-affinity penicillin-binding protein 5 gene in a clinical vancomycin-resistant Enterococcus faecium isolate
10. Use of an excision reporter plasmid to study the intracellular mobility of the conjugative transposon Tn916 in gram-positive bacteria
11. Circularization of Tn916 is required for expression of the transposon-encoded transfer functions: Characterization of long tetracycline-inducible transcripts reading through the attachment site
12. Genetic and molecular studies of a composite chromosomal element (Tn3705) containing a Tn916-modified structure (Tn3704) in Streptococcus anginosus F22
13. Sequence analysis of termini of conjugative transposon Tn916
14. Unconstrained bacterial promiscuity: The Tn916-Tn1545 family of conjugative transposons
15. Site-specific DNA binding using a variation of the double-stranded DNA binding motif
16. Characterization in vitro and in vivo of a new HU family protein from Streptococcus thermophilus ST11
17. The integrase family of tyrosine recombinases: Evolution of a conserved active site domain
18. Nucleotide sequence of the 18-kb conjugative transposon Tn916 from Enterococcus faecalis
19. Interactions between lambda Int molecules bound to sites in the region of strand exchange are required for efficient Holliday junction resolution
20. The Holliday junction intermediates of lambda integrative and excisive recombination respond differently to the bending proteins integration host factor and excisionase
21. Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: Strategy for targeting and cloning of genes from gram-positive bacteria
22. Deformation of DNA during site-specific recombination of bacteriophage lambda: Replacement of IHF protein by HU protein or sequence-directed bends
23. In vitro selection of integration host factor binding sites
24. Searching for and predicting the activity of sites for DNA binding proteins: Compilation and analysis of the binding sites for Escherichia coli integration host factor (IHF)
25. A functional origin of transfer (oriT) on the conjugative transposon Tn916
26. Dissecting the resolution reaction of lambda integrase using suicide Holliday junction substrates
27. An E. coli mutant unable to support site-specific recombination of bacteriophage lambda
28. Mapping of a higher order protein-DNA complex: Two kinds of long-range interactions in lambda attL
29. The complete genome sequence of the gram-positive bacterium Bacillus subtilis
30. Control of directionality in integrase-mediated recombination: Examination of recombination directionality factors (RDFs) including Xis and Cox proteins
31. Approaches to DNA mutagenesis: An overview
32. Tn916 integrase contains two independent DNA binding domains that recognize different DNA sequences
33. Tn916 target DNA sequences bind the C-terminal domain of integrase protein with different affinities that correlate with transposon insertion frequency
34. Conjugative transposon Tn916: Evidence for excision with formation of 5′-protruding termini
35. The joint of Tn916 circular intermediates is a homoduplex in Enterococcus faecalis
36. An efficient 1-tube PCR method for internal site-directed mutagenesis of large amplified molecules
37. Regulation of excision of the conjugative transposon Tn916
38. Excision of the conjugative transposon Tn916 in Lactococcus lactis
39. The DNA-binding protein HBsu is essential for normal growth and development in Bacillus subtilis
40. DNA looping generated by DNA bending protein IHF and the two domains of lambda integrase
41. A switch in the formation of alternative DNA loops modulates lambda site-specific recombination
42. Purification and properties of the Escherichia coli protein factor required for lambda integrative recombination
43. Characterization of the bacteriophage lambda excisionase (Xis) protein: The C terminus is required for Xis-integrase cooperativity but not for DNA binding
44. Site-specific recombination intermediates trapped with suicide substrates
45. Half-att site substrates reveal the homology independence and minimal protein requirements for productive synapsis in lambda excisive recombination
46. Histones, HMG, HU, IHF: Même combat
47. Characterization of the Tn916-like transposon Tn3872 in a strain of Abiotrophia defectiva (Streptococcus defectivus) causing sequential episodes of endocarditis in a child
48. Molecular characterization of two proteins involved in the excision of the conjugative transposon Tn1545: Homologies with other site-specific recombinases
49. Tn916 family conjugative transposons and dissemination of antimicrobial resistance determinants
50. Excision of a conjugative transposon in vitro by the Int and Xis proteins of Tn916
51. DNA binding by the Xis protein of the conjugative transposon Tn916
52. Conjugative transposons: An unusual and diverse set of integrated gene transfer elements
53. Conjugative transposition of Tn916: Preferred targets and evidence for conjugative transfer of a single strand and for a double-stranded circular intermediate
54. Conjugative transposition
55. Architectural flexibility in lambda site-specific recombination: Three alternate conformations channel the attL site into three distinct pathways
56. Characterization of the left 4 kb of conjugative transposon Tn916: Determinants involved in excision
57. Empirical estimation of protein-induced DNA bending angles: Application to λ site-specific recombination complexes
58. Protein-protein interactions in a higher-order structure direct lambda site-specific recombination
59. Cellular factors couple recombination with growth phase: Characterization of a new component in the lambda site-specific recombination pathway
60. Mutations in an integration host factor-binding site: Effect on lambda site-specific recombination and regulatory implications
61. A new type of conjugative transposon encodes resistance to sulfamethoxazole, trimethoprim, and streptomycin in Vibrio cholerae O139
62. NMR structure of the Tn916 integrase-DNA complex
63. Interaction of the lambda site-specific recombination protein Xis with attachment site DNA
64. Cloning and sequencing of the hup gene encoding the histone-like protein HS1 of Streptomyces lividans