NMR structure of the Tn916 integrase-DNA complex

Nature Structural Biology

Volumn 6, Issue 4, 1999, Pages 366-373

  Wojciak, Jonathan M ^a   Connolly, Kevin M ^a   Clubb, Robert T ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INTEGRASE;

ARTICLE; BINDING SITE; DNA BINDING; ENZYME ACTIVITY; ENZYME MECHANISM; FLUORESCENCE SPECTROSCOPY; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; STRUCTURE ANALYSIS; TRANSPOSON;

BINDING SITES; DNA; DNA TRANSPOSABLE ELEMENTS; INTEGRASES; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; PROTEIN CONFORMATION; SOLUTIONS; SPECTROMETRY, FLUORESCENCE;

EID: 0032920469     PISSN: 10728368     EISSN: None     Source Type: Journal    
DOI: 10.1038/7603     Document Type: Article

References (16)

1. Clewell, D.E., Flannagan, S.E. & Jaworski, D.D. Unconstrained bacterial promiscuity: the Tn916-Tn1545 family of conjugative transposons. Trends Microbiol. 3, 229-236 (1995).
2. Scott, J.R. & Churchward, G. Conjugative transposition. Ann. Rev. Microbiol. 49, 367-397 (1995).
3. Salyers, A.A., Shoemaker, N.B., Stevens, A.M. & Li, LY. Conjugative transposons: an unusual and diverse set of integrated gene transfer elements. Microbiol. Rev. 59, 579-590 (1995).
4. Poyart-Salmeron. C., Trieu-Cuot, P., Carlier, C. & Courvalin, P. Molecular characterization of two proteins involved in the excision of the conjugative transposon Tn1545. EMBO J. 8, 2425-2433 (1989).
5. Rudy, C., Taylor, K.L., Hinerfeld, D., Scott, J.R. & Churchward, G. Excision of a conjugative transposon in vitro by the Int and Xis proteins of Tn916. Nucleic Acids Res. 25, 4061-4066 (1997).
6. Jaworski, D.D., Flannagan, S.E. & Clewell, D.B. Analyses of traA. int-Tn, and xis-Tn mutations in the conjugative transposon Tn916 Enterococcus faecalis. Plasmid 36, 201-208 (1996).
7. Esposito, D. & Scocca, J.J. The integrase family of tyrosine recombinases: evolution of a conserved active site domain. Nucleic Acid Res. 25, 3605-3614 (1997).
8. Argos, P. et al. The integrase family of site specific recombinases: regional similarities and global diversity. EMBO J. 5, 433-440 (1986).
9. Lu, F. & Churchward, G. Tn916 integrase contains two independent DMA binding domains that recognize different DNA sequences. EMBO J. 13, 1541-1548 (1994).
10. Landy, A. Dynamic, structural, and regulatory aspects of lambda site-specific integration. Ann. Rev. Biochem. 58, 913-949 (1989).
11. Gou, F., Gopaul, D.N. & Van Duyne, G.D. Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse. Nature 389, 40-46 (1997).
12. Hickman, A.B., Waninger, S., Scocca, J.J. & Dyda, F. Molecular organization in site-specific recombination: the catalytic domain of bacteriophage HP1 at 2.7Å resolution. Cell 89, 227-237 (1997).
13. Kwon, H.J., Tirumalai, R., Landy, A. & Ellenberger, T. Flexibility in DNA recombination: structure of the lambda integrase catalytic core. Science 276, 126-131 (1997).
14. Subramanya, H.S. et al. Crystal structure of the site-specific recombinase, XerD. EMBO J. 16, 5178-5187 (1997).
15. Connolly, K.M., Wojciak. J.M. & Clubb, R.T. Site-specific DNA binding using a variation of the double stranded RNA binding motif. Nat Struct. Biol. 5, 546-550 (1998).
16. Harrison, S.C. A structural taxonomy of DNA-binding domains. Nature 353, 715-719 (1991).