DNA binding by the Xis protein of the conjugative transposon Tn916

Journal of Bacteriology

Volumn 179, Issue 8, 1997, Pages 2567-2572

  Rudy, Christine K ^a   Scott, June R ^a   Churchward, Gordon ^a  

^a EMORY UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEOPROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; ESCHERICHIA COLI; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN PURIFICATION; TRANSPOSON;

EID: 0031000745     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.8.2567-2572.1997     Document Type: Article

References (38)

1. Abremski, K., and S. Gottesman. 1981. Site-specific recombination: Xis-independent excisive recombination of bacteriophage lambda. J. Mol. Biol. 153:67-78.
2. Argos, P., A. Landy, K. Abremski, J. B. Egan, E. Haggard-Ljungquist, R. H. Hoess, M. J. Kahn, W. Kalionis, S. V. L. Narayana, L. S. Pierson, N. Sternberg, and J. M. Leong. 1986. The integrase family of site-specific recombinase: regional similarity and global diversity. EMBO J. 5:433-440.
3. Bringel, F. G., G. L. Van Alstine, and J. R. Scott. 1992. Conjugative transposition of Tn916: the transposon int gene is required only in the donor. J. Bacteriol. 174:4036-4041.
4. Bushman, W., S. Yin, L. L. Thio, and A. Landy. 1984. Determinants of directionality in lambda site-specific recombination. Cell 39:699-706.
5. Buu-Hoi, A., and T. Horodniceanu. 1980. Conjugative transfer of multiple antibiotic resistance markers of Streptococcus pneumoniae. J. Bacteriol. 143:313-320.
6. Caparon, M. G., and J. R. Scott. 1989. Excision and insertion of the conjugative transposon Tn916 involves a novel recombination mechanism. Cell 59:1027-1034.
7. Clewell, D. B., and S. E. Flannagan. 1993. The conjugative transposons of gram-positive bacteria, p. 369-393. In D. B. Clewell (ed.), Bacterial conjugation. Plenum Publishing Corp., New York, N.Y.
8. Clewell, D. B., S. E. Flannagan, Y, Ike, J. M. Jones, and C. Gawron-Burke. 1988. Sequence analysis of termini of conjugative transposon Tn916. J. Bacteriol. 170:3046-3052.
9. Clewell, D. B., S. E. Flannagan, and D. D. Jaworski. 1995. Unconstrained bacterial promiscuity: the Tn916-Tn1545 family of conjugative transposons. Trends Microbiol. 3:229-236.
10. Courvalin, P., and C. Carlier. 1986. Transposable multiple antibiotic resistance in Streptococcus pneumoniae. Mol. Gen. Genet. 205:291-297.
11. De Vargas, L. M., and A. Landy. 1991. A switch in the formation of alternative DNA loops modulates λ site-specific recombination. Proc. Natl. Acad. Sci. USA 88:588-592.
12. Flannagan, S. E., L. A. Zitzow, Y. A. Su, and D. B. Clewell. 1994. Nucleotide sequence of the 18-kb conjugative transposon Tn916 from Enterococcus faecalis. Plasmid 32:350-354.
13. Franke, A. E., and D. B. Clewell. 1981. Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of conjugative transfer in the absence of a conjugative plasmid. J. Bacteriol. 145:494-502.
14. Franz, B., and A. Landy. 1995. The Holliday junction intermediates of λ integrative and excisive recombination respond differently to the bending proteins integration host factor and excisionase. EMBO J. 14:397-406.
15. Gottesman, S. 1990. Minimizing proteolysis in Escherichia coli: genetic solutions. Methods Enzymol. 185:119-129.
16. Ike, Y., S. E. Flannagan, and D. B. Clewell. 1992. Hyperhemolytic phenomena associated with insertions of Tn916 into the hemolysis determinant of Enterococcus faecalis plasmid pAD1. J. Bacteriol. 174:1801-1809.
17. Kim, S., L. M. de Vargas, S. E. Nunes-Duby, and A. Landy. 1990. Mapping of a higher order protein-DNA complex: two kinds of long-range interactions in λ attL. Cell 63:773-781.
18. Kim, S., and A. Landy. 1992. Lambda Int protein bridges between higher order complexes at two distant chromosomal loci attL and attR. Science 256:198-203.
19. Landy, A. 1989. Dynamic, structural, and regulatory aspects of λ site-specific recombination. Annu. Rev. Biochem. 58:913-949.
20. Lu, F., and G. Churchward. 1994. Conjugative transposition: Tn916 integrase contains two independent DNA binding domains that recognize different DNA sequences. EMBO J. 13:1541-1548.
21. Lu, F., and G. Churchward. 1995. Tn916 target DNA sequences bind the C-terminal domain of integrase protein with different affinities that correlate with transposon insertion frequency. J. Bacteriol. 177:1938-1946.
22. Manganelli, R., S. Ricci, and G. Pozzi. 1996. Conjugative transposon Tn916: evidence for excision with formation of 5′-protruding termini. J. Bacteriol. 178:5813-5816.
23. Numrych, T. E., R. I. Gumport, and J. F. Gardner. 1992. Characterization of the bacteriophage lambda excisionase (Xis) protein: the C-terminus is required for Xis-integrase cooperatively but not for DNA binding. EMBO J. 11:3797-3806.
24. Poyart-Salmeron, C., P. Trieu-Cuot, C. Carlier, and P. Courvalin. 1990. The integration-excision system of the conjugative transposon Tn1545 is structurally and functionally related to those of the lambdoid phages. Mol. Microbiol. 4:1513-1521.
25. Poyart-Salmeron, C., P. Trieu-Cuot, C. Carlier, and P. Courvalin. 1989. Molecular characterization of two proteins involved in the excision of the conjugative transposon Tn1545: homologies with other site-specific recombinases. EMBO J. 8:2425-2433.
26. Rauch, P. J. G., and W. M. de Vos. 1992. Characterization of the novel nisin-sucrose conjugative transposon Tn5276 and its insertion in Lactococcus lactis. J. Bacteriol. 174:1280-1287.
27. Rauch, P. J. G., and W. M. de Vos. 1994. Identification and characterization of genes involved in excision of the Lactococcus lactis conjugative transposon Tn5276. J. Bacteriol. 176:2165-2171.
28. Rudy, C. K., and J. R. Scott. 1994. Length of coupling sequence of Tn916. J. Bacteriol. 176:3386-3388.
29. Scott, J. R. 1993. Conjugative transposons, p. 597-614. In A. L. Sonenshein, J. A. Hoch, and R. Losick (ed.), Bacillus subtilis and other gram-positive bacteria. American Society for Microbiology, Washington, D.C.
30. Scott, J. R. 1992. Sex and the single circle: conjugative transposition. J. Bacteriol. 174:6005-6010.
31. Scott, J. R., F. Bringel, D. Marra, G. Van Alstine, and C. K. Rudy. 1994. Conjugative transposition of Tn916: preferred targets and evidence for conjugative transfer of a single strand and for a double-stranded circular intermediate. Mol. Microbiol. 11:1099-1108.
32. Scott, J. R., and G. G. Churchward. 1995. Conjugative transposition. Annu. Rev. Microbiol. 49:367-397.
33. Scott, J. R., P. A. Kirchman, and M. G. Caparon. 1988. An intermediate in the transposition of the conjugative transposon Tn916. Proc. Natl. Acad. Sci. USA 85:4809-4813.
34. Senghas, E., J. M. Jones, M. Yamamoto, C. Garwon-Burke, and D. B. Clewell. 1988. Genetic organization of the bacterial conjugative transposon Tn916. J. Bacteriol. 170:245-249.
35. Su, Y. A., and D. B. Clewell. 1993. Characterization of the left 4 kb of conjugative transposon Tn916: determinants involved in excision. Plasmid 30:234-250.
36. Taylor, K. L., and G. G. Churchward. 1997. Specific DNA cleavage mediated by the integrase of conjugative transposon Tn916. J. Bacteriol. 179:1117-1125.
37. Thompson, J. F., and A. Landy. 1988. Empirical estimation of protein-induced DNA bending angles: applications to λ site-specific recombination complexes. Nucleic Acids Res. 16:9687-9705.
38. Yin, S., W. Bushman, and A. Landy. 1985. Interaction of the λ site-specific recombination protein Xis with attachment site DNA. Proc. Natl. Acad. Sci. USA 82:1040-1044.