Fine structure and activity of discrete RAG-HMG complexes on V(D)J recombination signals

Molecular and Cellular Biology

Volumn 22, Issue 5, 2002, Pages 1340-1351

  Swanson, Patrick C ^a  

^a CREIGHTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CURVED DNA; HIGH MOBILITY GROUP PROTEIN; RAG1 PROTEIN; RAG2 PROTEIN;

ARTICLE; COMPLEX FORMATION; DNA FOOTPRINTING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DNA BINDING; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE;

BASE SEQUENCE; DIMERIZATION; DNA; DNA-BINDING PROTEINS; GENE REARRANGEMENT, T-LYMPHOCYTE; HIGH MOBILITY GROUP PROTEINS; HMGB1 PROTEIN; HMGB2 PROTEIN; HOMEODOMAIN PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; PROTEIN CONFORMATION; RECOMBINATION, GENETIC; TRANSPOSASES;

EID: 0036171148     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.22.5.1340-1351.2002     Document Type: Article

References (36)

1. The RAG1 homeodomain recruits HMG1 and HMG2 to facilitate recombination signal sequence binding and to enhance the intrinsic DNA-bending activity of RAG1-RAG2
2. Distinct roles of RAG1 and RAG2 in binding the V(D)J recombination signal sequences
3. Hairpin coding end opening is mediated by RAG1 and RAG2 proteins
4. Flexing DNA: HMG-box proteins and their partners
5. RAG1 mediates signal sequence recognition and recruitment of RAG2 in V(D)J recombination
6. Detection of RAG protein-V(D)J recombination signal interactions near the site of DNA cleavage by UV cross-linking
7. Mechanism for specificity by HMG-1 in enhanceosome assembly
8. The MIDAS display system
9. The RAG proteins and V(D)J recombination: Complexes, ends, and transposition
10. The high mobility group protein HMG1 can reversibly inhibit class II gene transcription by interaction with the TATA-binding protein
11. The mechanism of V(D)J joining: Lessons from molecular, immunological, and comparative analyses
12. RAG-1 and RAG-2-dependent assembly of functional complexes with V(D)J recombination substrates in solution
13. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps
14. A highly ordered structure in V(D)J recombination cleavage complexes is facilitated by HMG1
15. RAG1 and RAG2 cooperate in specific binding to the recombination signal sequence in vitro
16. Footprint analysis of the RAG protein recombination signal sequence complex for V(D)J type recombination
17. RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination
18. The 3′ to 5′ exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks
19. Nbs1 potentiates ATP-driven DNA unwinding and endonuclease cleavage by the Mre11/Rad50 complex
20. A dimer of the lymphoid protein RAG1 recognizes the recombination signal sequence and the complex stably incorporates the high mobility group protein HMG2
21. V(D)J recombination: Broken DNA molecules with covalently sealed (hairpin) coding ends in scid mouse thymocytes
22. Characterization of broken DNA molecules associated with V(D)J recombination
23. The RAG proteins in V(D)J recombination: More than just a nuclease
24. The V(D)J recombination activating gene, RAG-1
25. Double-strand signal sequence breaks in V(D)J recombination are blunt, 5′-phosphorylated, RAG-dependent, and cell cycle regulated
26. DNA hairpin opening mediated by the RAG1 and RAG2 proteins
27. The homeodomain region of Rag-1 reveals the parallel mechanisms of bacterial and V(D)J recombination
28. The DDE motif in RAG-1 is contributed in trans to a single active site that catalyzes the nicking and transesterification steps of V(D)J recombination
29. RAG-2 promotes heptamer occupancy by RAG-1 in the assembly of a V(D)J initiation complex
30. V(D)J recombination signal recognition: Distinct, overlapping DNA-protein contacts in complexes containing RAG1 with and without RAG2
31. HMG1 and 2, and related 'architectural' DNA-binding proteins
32. Stimulation of V(D)J cleavage by high mobility group proteins
33. Similarities between initiation of V(D)J recombination and retroviral integration
34. Specific photocrosslinking of DNA-protein complexes: Identification of contacts between integration host factor and its target DNA
35. The nicking step in V(D)J recombination is independent of synapsis: Implications for the immune repertoire
36. High mobility group protein 2 functionally interacts with the POU domains of octamer transcription factors