Polylysine and polyornithine gene transfer complexes: A study of complex stability and cellular uptake as a basis for their differential in-vitro transfection efficiency

Journal of Drug Targeting

Volumn 10, Issue 1, 2002, Pages 1-9

  Ramsay, E ^a   Gumbleton, M ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

DNA condensation; Flow cytometry; Gene transfer; Polylysine; Polyornithine

Indexed keywords

HEPARIN; PLASMID DNA; POLY DEXTRO LYSINE; POLYANION; POLYASPARTIC ACID; POLYCATION; POLYLYSINE; POLYORNITHINE;

ARTICLE; BINDING AFFINITY; COMPLEX FORMATION; CONTROLLED STUDY; DATA ANALYSIS; DRUG EFFICACY; DRUG STABILITY; DRUG UPTAKE; ENZYMATIC DEGRADATION; GENE DISRUPTION; GENE TRANSFER; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; NONVIRAL GENE DELIVERY SYSTEM; PHARMACOLOGICAL PARAMETERS; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; SPECTROFLUOROMETRY;

CELL LINE; DEOXYRIBONUCLEASE I; DNA; DRUG CARRIERS; ELECTROPHORESIS, AGAR GEL; ETHIDIUM; FLOW CYTOMETRY; FLUORESCEIN; FLUORESCENT DYES; HUMANS; PEPTIDES; POLYLYSINE; SPECTROMETRY, FLUORESCENCE; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0036119212     PISSN: 1061186X     EISSN: None     Source Type: Journal    
DOI: 10.1080/10611860290007487     Document Type: Article

References (34)

1. "Stability of peptide-condensed plasmid DNA formulations"
2. 2 group on the side chain on the conformation of the poly-α-amino acids"
3. "DNA condensation"
4. "Poly-L-ornithine-mediated transformation of mammalian cells"
5. "Enhanced resistance to nuclease degradation of nucleic acids complexed to asialoglycoprotein-polylysine carriers"
6. "Target specific optimization of cationic lipid-based systems for pulmonary gene therapy"
7. "Multiple range and multiple F tests"
8. "The reduction of the positive charges of polylysine by partial gluconolyation increases the transfection efficiency of polylysine/DNA complexes"
9. "Mode of formation and structural features of DNA-cationic liposome complexes used for transfection"
10. "Polyamidoamine cascade polymers mediate efficient transfection of cells in culture"
11. "Interaction of plasmid DNA with polypeptides and DNA transfection activity"
12. "High efficiency of animal cell DNA transfection using poly-L-ornithine in optimized conditions"
13. "Pharmaceutical and biological properties of poly(amino acid)/DNA polyplexes"
14. "Biochemical characterisation of polycation-induced cytotoxicity to human vascular endothelial cells"
15. "Gene transfer in vivo: Sustained expression and regulation of genes introduced into the liver by receptor-targeted uptake"
16. "An evaluation of receptor-mediated gene transfer using synthetic DNA-ligand complexes"
17. "Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells"
18. "Polycation-DNA complexes for gene delivery: A comparison of the biopharmaceutical properties of cationic polypeptides and cationic lipids"
19. "Examination of the biophysical interaction between plasmid DNA and the polycations, polylysine and polyornithine, as a basis for their differential gene transfection in-vitro"
20. "Chiral and structural discrimination in binding of polypeptides with condensed nucleic acid structures"
21. "Gene transfer with lipospermines and polyethylenimines"
22. "Receptor-mediated endocytosis and nuclear transport of a transfecting DNA construct"
23. "Deoxyribonucleic acid-polylysine complexes. Structure and nucleotide specificity"
24. "Absorption and fluorescence properties of fluorescein"
25. "Cell line A549: A model system for the study of alveolar type II cell function"
26. "Protamine sulfate enhances lipid-mediated gene transfer"
27. "The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes"
28. "Condensation of plasmid DNA with polylysine improves liposome-mediated gene transfer into established and primary muscle cells"
29. "Transferrin-polycation-DNA complexes: The effect of polycations on the structure of the complex and DNA delivery to cells"
30. "Influenza virus haemaglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: Towards a synthetic virus-like gene transfer vehicle"
31. "Chloroquine and amphipathic peptide helices show synergistic transfection in vitro"
32. "Receptor-mediated gene delivery and expression in vivo"
33. "Polylysine-based transfection systems utilizing receptor-mediated delivery"