Hepatitis C virus NS5A colocalizes with the core protein on lipid droplets and interacts with apolipoproteins

Virology

Volumn 292, Issue 2, 2002, Pages 198-210

  Shi, Stephanie T ^a   Polyak, Stephen J ^b   Tu, Hong ^a   Taylor, Deborah R ^a   Gretch, David R ^b   Lai, Michael M C ^a  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Apolipoprotein; Core; Hepatitis C virus; Lipid droplets; NS5A

Indexed keywords

APOLIPOPROTEIN A1; CORE PROTEIN; FAT DROPLET; HIGH DENSITY LIPOPROTEIN; NONSTRUCTURAL PROTEIN 5A; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ARTICLE; CELL MEMBRANE; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DNA LIBRARY; ENDOPLASMIC RETICULUM; FLOTATION; GOLGI COMPLEX; HEPATITIS C VIRUS; HEPATOMA CELL; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IMMUNOPRECIPITATION; LIPID METABOLISM; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; TWO HYBRID SYSTEM;

HEPATITIS C VIRUS;

EID: 0036060095     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.2001.1225     Document Type: Article

References (78)

1. Hepatitis C virus and other flaviviridae viruses enter cells via low density lipoprotein receptor
2. Hepatic steatosis: A specific sign of hepatitis C reinfection after liver transplantation
3. Rapid movement of newly synthesized chicken apolipoprotein AI to trans-Golgi network and its secretion in Madin-Darby canine kidney cells
4. Hepatitis C virus core protein shows a cytoplasmic localization and associates to cellular lipid storage droplets
5. Perilipin is located on the surface layer of intracellular lipid droplets in adipocytes
6. Efficient initiation of HCV RNA replication in cell culture
7. Overexpression of human apolipoprotein A-II in mice induces hypertriglyceridemia due to defective very low density lipoprotein hydrolysis
8. Adipose differentiation-related protein is an ubiquitously expressed lipid storage droplet-associated protein
9. Perilipin A increases triacylglycerol storage by decreasing the rate of triacylglycerol hydrolysis
10. Structural models of human apolipoprotein A-I
11. Lipid droplets: Proteins floating on a pool of fat
12. Nonstructural protein 5A of hepatitis C virus inhibits the function of karyopherin beta3
13. Hepatitis C virus nonstructural protein 5A contains potential transcriptional activator domains
14. The role of apolipoprotein AI domains in lipid binding
15. Mutations in the nonstructural protein 5A gene and response to interferon in patients with chronic hepatitis C virus 1b infection
16. Analyzing the mechanisms of interferon-induced apoptosis using CrmA and hepatitis C virus NS5A
17. Apolipoprotein A-containing lipoprotein particles: Physiological role, quantification, and clinical significance
18. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase
19. Steatosis and intrahepatic hepatitis C virus in chronic hepatitis
20. Caveolin-2 is targeted to lipid droplets, a new "membrane domain" in the cell
21. Mutations in the interferon-sensitivity determining region of hepatitis C virus and transcriptional activity of the nonstructural region 5A protein
22. Antiapoptotic and oncogenic potentials of hepatitis C virus are linked to interferon resistance by viral repression of the PKR protein kinase
23. Repression of the PKR protein kinase by the hepatitis C virus NS5A protein: A potential mechanism of interferon resistance
24. Hepatitis C virus NS5A protein protects against TNF-alpha mediated apoptotic cell death
25. Hepatitis C virus NS5A protein modulates transcription through a novel cellular transcription factor SRCAP
26. Hepatitis C virus NS5A protein modulates cell cycle regulatory genes and promotes cell growth
27. Comparison of the cytolytic effects in vitro on Trypanosoma brucei brucei of plasma, high density lipoproteins, and apolipoprotein A-I from hosts both susceptible (cattle and sheep) and resistant (human and baboon) to infection
28. Expression and identification of hepatitis C virus polyprotein cleavage products
29. Apolipoprotein E localization in rat hepatocytes by immunogold labeling of cryothin sections
30. The influence of apolipoproteins on the hepatic lipase-mediated hydrolysis of high density lipoprotein phospholipid and triacylglycerol
31. Evidence that apolipoprotein A-I facilitates hepatic lipase-mediated phospholipid hydrolysis in reconstituted HDL containing apolipoprotein A-II
32. Sequence motifs required for lipid droplet association and protein stability are unique to the hepatitis C virus core protein
33. Characterization of the nuclear localization signal and subcellular distribution of hepatitis C virus nonstructural protein NS5A
34. Properties of an N-terminal proteolytic fragment of apolipoprotein AI in solution and in reconstituted high density lipoproteins
35. Apolipoprotein A-I structure lipid properties in homogeneous reconstituted spherical and discoidal high density lipoproteins
36. Apolipoprotein is responsible for neutralization of xenotropic type C virus by mouse serum
37. Hepatitis C virus nonstructural region 5A protein is a potent transcriptional activator
38. Subcellular localization of hepatitis C viral proteins in mammalian cells
39. Processing pathways of the hepatitis C virus proteins
40. Hepatitis C virus NS5A physically associates with p53 and regulates p21/waf1 gene expression in a p53-dependent manner
41. Homotypic interaction and multimerization of hepatitis C virus core protein
42. Hepatitis B virus surface antigen binds to apolipoprotein H
43. The core protein of hepatitis C virus induces hepatocellular carcinoma in transgenic mice
44. Hepatitis C virus core protein induces hepatic steatosis in transgenic mice
45. Inhibition of lymphocyte proliferation stimulated by lectins and allogeneic cells by normal plasma lipoproteins
46. Apolipoprotein A-II influences the substrate properties of human HDL2 and HDL3 for hepatic lipase
47. Different reactivities of high density lipoprotein2 subfractions with hepatic lipase
48. An Hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein
49. Accumulation of caveolin in the endoplasmic reticulum redirects the protein to lipid storage droplets
50. Apolipoprotein A-I and its amphipathic helix peptide analogues inhibit human immunodeficiency virus-induced syncytium formation
51. Characterization of cell lines carrying self-replicating hepatitis C virus RNAs
52. A caveolin dominant negative mutant associates with lipid bodies and induces intracellular cholesterol imbalance
53. Hepatitis C virus nonstructural 5A protein induces interleukin-8, leading to partial inhibition of the interferon-induced antiviral response
54. Characterization of the effects of hepatitis C virus nonstructural 5A protein expression in human cell lines and on interferon-sensitive virus replication
55. Apolipoprotein A1 and hepatic fibrosis
56. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O
57. Hepatitis C virus core protein binds to apolipoprotein AII and its secretion is modulated by fibrates
58. Sendai virus M protein binds independently to either the F or the HN glycoprotein in vivo
59. Cleavage of hepatitis C virus nonstructural protein 5A by a caspase-like protease(s) in mammalian cells
60. Carboxyl-terminal domain truncation alters apolipoprotein A-I in vivo catabolism
61. The amphipathic helix in the exchangeable apolipoproteins: A review of secondary structure and function
62. Expression, identification and subcellular localization of the proteins encoded by the hepatitis C viral genome
63. Comparison of the human apolipoprotein genes. Apo AII presents a unique functional intron-exon junction
64. Effect of apolipoproteins on the hepatic lipase-catalyzed hydrolysis of human plasma high density lipoprotein2-triacylglycerols
65. Changes in blood lipid composition and response to interferon treatment in chronic hepatitis C
66. Antiviral effects of apolipoprotein A-I and its synthetic amphipathic peptide analogs
67. 2+ stores during phagocytosis in human neutrophils
68. NS5A, a nonstructural protein of hepatitis C virus, binds growth factor receptor-bound protein 2 adaptor protein in a Src homology 3 domain/ligand-dependent manner and perturbs mitogenic signaling
69. The amino terminal deletion mutants of hepatitis C virus nonstructural protein NS5A function as transcriptional activators in yeast
70. Phosphorylation of hepatitis C virus-encoded nonstructural protein NS5A
71. Density heterogeneities of hepatitis C virus in human sera due to the binding of beta-lipoproteins and immunoglobulins
72. Hepatitis C virus RNA polymerase and NS5A complex with a SNARE-like protein
73. Caveolin, cholesterol, and lipid droplets?
74. 'Coatomer': A cytosolic protein complex containing subunits of non-clathrin-coated Golgi transport vesicles
75. ApoA-II maintains HDL levels in part by inhibition of hepatic lipase. Studies In apoA-II and hepatic lipase double knockout mice
76. TIP47 associates with lipid droplets
77. Characterization of hepatitis C virus (HCV) and HCV E2 interactions with CD81 and the low-density lipoprotein receptor
78. Human ApoA-II inhibits the hydrolysis of HDL triglyceride and the decrease of HDL size induced by hypertriglyceridemia and cholesteryl ester transfer protein in transgenic mice