The genes for perforin, granzymes A-C and IFN-γ are differentially expressed in single CD8+ T cells during primary activation

International Immunology

Volumn 14, Issue 6, 2002, Pages 605-613

  Kelso, Anne ^a,b,d   Costelloe, Elaine O ^a,b   Johnson, Barbara J ^a,b   Groves, Penny ^a   Buttigieg, Kathy ^a   Fitzpatrick, David R ^a,c  

^a QIMR BERGHOFER MEDICAL RESEARCH INSTITUTE   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

^c AMGEN INC   (United States)

^d ROYAL BRISBANE AND WOMEN'S HOSPITAL   (Australia)

Author keywords

Cytotoxic T lymphocyte; IFN ; Single cell analysis

Indexed keywords

CD3 ANTIGEN; CD8 ANTIGEN; GAMMA INTERFERON; GRANZYME A; GRANZYME B; GRANZYME C; HERMES ANTIGEN; INTERLEUKIN 2; LYMPHOCYTE FUNCTION ASSOCIATED ANTIGEN 1; MESSENGER RNA; MONOCLONAL ANTIBODY; PADGEM PROTEIN; PERFORIN; SERINE PROTEINASE; UNCLASSIFIED DRUG; GRANZYME; GZMB PROTEIN, MOUSE; GZMC PROTEIN, MOUSE; MEMBRANE PROTEIN; PORE FORMING CYTOTOXIC PROTEIN;

ANIMAL CELL; ARTICLE; CELL CLONE; CELL SUBPOPULATION; CONTROLLED STUDY; CYTOLYSIS; FEMALE; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENETIC REGULATION; HEREDITY; LYMPH NODE; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STATISTICAL ANALYSIS; T LYMPHOCYTE ACTIVATION; ANIMAL; C57BL MOUSE; CD8+ T LYMPHOCYTE; GENETICS; IMMUNOLOGY; IN VITRO STUDY; KINETICS; LYMPHOCYTE ACTIVATION; METABOLISM;

ANIMALS; CD8-POSITIVE T-LYMPHOCYTES; CLONE CELLS; FEMALE; GENE EXPRESSION; GRANZYMES; INTERFERON TYPE II; KINETICS; LYMPHOCYTE ACTIVATION; MEMBRANE GLYCOPROTEINS; MICE; MICE, INBRED C57BL; POLYMERASE CHAIN REACTION; PORE FORMING CYTOTOXIC PROTEINS; RNA, MESSENGER; SERINE ENDOPEPTIDASES;

EID: 0035988243     PISSN: 09538178     EISSN: None     Source Type: Journal    
DOI: 10.1093/intimm/dxf028     Document Type: Article

References (50)

1. Cytotoxicity mediated by T cells and natural killer cells is greatly impaired in perforin-deficient mice
2. Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways
3. Immune function in mice lacking the perforin gene
4. Unlocking the secrets of cytotoxic granule proteins
5. Granzyme B (GraB) autonomously crosses the cell membrane and perforin initiates apoptosis and GraB nuclear localization
6. Cytosolic delivery of granzyme B by bacterial toxins: Evidence that endosomal disruption, in addition to transmembrane pore formation, is an important function of perforin
7. Cytotoxic lymphocytes require granzyme B for the rapid induction of DNA fragmentation and apoptosis in allogeneic target cells
8. Granzyme A loading induces rapid cytolysis and a novel form of DNA damage independently of caspase activation
9. Induction of rapid histone degradation by the cytotoxic T lymphocyte protease Granzyme A
10. Granzyme A initiates an alternative pathway for granule-mediated apoptosis
11. Granzyme A is an interleukin 1β-converting enzyme
12. Nephritogenic T cells use granzyme C as a cytotoxic mediator
13. In vitro- and ex vivo-derived cytolytic leukocytes from granzyme A × B double knockout mice are defective in granule-mediated apoptosis but not lysis of target cells
14. Granzymes are the essential downstream effector molecules for the control of primary virus infections by cytolytic leukocytes
15. Granzyme A and B-deficient killer lymphocytes are defective in eliciting DNA fragmentation but retain potent in vivo anti-tumor capacity
16. Granzyme A-deficient mice retain potent cell-mediated cytotoxicity
17. Granzyme A is critical for recovery of mice from infection with the natural cytopathic viral pathogen, ectromelia
18. + T cells by perforin
19. A role for perforin in downregulating T-cell responses during chronic viral infection
20. + T cell homeostasis by perforin and interferon-γ
21. Cell specificity of granzyme gene expression
22. In vivo primed mouse T cells selectively express T cell-specific serine proteinase-1 and the proteinase-like molecules granzyme B and C
23. CTLA-1 and CTLA-3 serine esterase transcripts are detected mostly in cytotoxic T cells, but not only and not always
24. Quantitative polymerase chain reaction analysis of cytotoxic cell proteinase gene transcripts in T cells. Pattern of expression is dependent on the nature of the stimulus
25. + cells induced by protein immunization
26. + T cells
27. + T cell can give rise to progeny expressing type 1 and/or type 2 cytokine genes and can retain its multipotentiality through many cell divisions
28. FAS is highly expressed in the germinal center but is not required for regulation of the B-cell response to antigen
29. + T cells and induces synthesis of IL-3 and IFN-γ but not IL-4 or IL-6
30. A clonal culture system demonstrates that interleukin 4 induces a subpopulation of noncytolytic T cells with low CD8, perforin and granzyme expression
31. Concanamycin A, a powerful tool for characterization and estimation of contribution of perforin-and Fas-based lytic pathways in cell-mediated cytotoxicity
32. Inactivation and proteolytic degradation of perforin within lytic granules upon neutralization of acidic pH
33. Enumeration of lymphokine mRNA-containing cells in vivo in a murine graft-versus-host reaction using the PCR
34. + T cells activated in a T helper type 2-like response in vivo
35. + T cell populations
36. Interleukin 2 is both necessary and sufficient for the growth and differentiation of lectin-stimulated cytolytic T lymphocyte precursors
37. + T cells with Drosophila cell transfectants as antigen presenting cells
38. + T cells activated in vitro with MHC class I binding immunogenic peptides and IL-2 in the absence of specialized APCs
39. T and B memory cells
40. Organization of the gene encoding the mouse T-cell-specific serine proteinase 'granzyme A'
41. A comparison of the flanking regions of the mouse cytotoxic cell proteinase genes
42. Appearance of granule-associated molecules during activation of cytolytic T-lymphocyte precursors by defined stimuli
43. Expression of perforin and granzymes in vivo: Potential diagnostic markers for activated cytotoxic cells
44. Long-range disruption of gene expression by a selectable marker cassette
45. Genomic organization of the mouse pore-forming protein (perforin) gene and localization to chromosome 10. Similarities to and differences from C9
46. Perforin is activated by a proteolytic cleavage during biosynthesis which reveals a phospholipid-binding C2 domain
47. Generation of active myeloid and lymphoid granule serine proteases requires processing by the granule thiol protease dipeptidyl peptidase I
48. Dipeptidyl peptidase I is required for the processing and activation of granzymes A and B in vivo
49. Functional diversity of helper T lymphocytes
50. + T cells