In vitro- and ex vivo-derived cytolytic leukocytes from granzyme A X B double knockout mice are defective in granule-mediated apoptosis but not lysis of target cells

Journal of Experimental Medicine

Volumn 186, Issue 10, 1997, Pages 1781-1786

  Simon, Markus M ^a   Hausmann, Michael ^a   Tran, Thao ^a   Ebnet, Klaus ^a,d   Tschopp, Jürg ^b   ThaHla, Ron ^c   Müllbacher, Arno ^c  

^a MAX PLANCK INSTITUTE OF IMMUNOBIOLOGY AND EPIGENETICS   (Germany)

^b UNIVERSITY OF LAUSANNE   (Switzerland)

^c Biology and Environment   (Australia)

^d UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMIUM 51; DNA; GRANZYME A; GRANZYME B; IODINE 125; PERFORIN;

ANIMAL CELL; ANIMAL EXPERIMENT; APOPTOSIS; ARTICLE; CYTOLYSIS; CYTOTOXIC T LYMPHOCYTE; IMMUNOPATHOGENESIS; INFLUENZA VIRUS A; MOUSE; NATURAL KILLER CELL; NONHUMAN; PRIORITY JOURNAL; TARGET CELL;

ANIMALS; APOPTOSIS; CYTOPLASMIC GRANULES; CYTOTOXICITY TESTS, IMMUNOLOGIC; CYTOTOXICITY, IMMUNOLOGIC; DNA FRAGMENTATION; FEMALE; GRANZYMES; LEUKEMIA L1210; LYMPHOMA; MALE; MAST-CELL SARCOMA; MEMBRANE GLYCOPROTEINS; MICE; MICE, INBRED BALB C; MICE, INBRED C57BL; MICE, KNOCKOUT; PORE FORMING CYTOTOXIC PROTEINS; SERINE ENDOPEPTIDASES; T-LYMPHOCYTES, CYTOTOXIC; TUMOR CELLS, CULTURED;

EID: 0030723209     PISSN: 00221007     EISSN: None     Source Type: Journal    
DOI: 10.1084/jem.186.10.1781     Document Type: Article

References (36)

1. +-independent T cell-mediated cytotoxicity. J. Exp. Med. 177:195-200.
2. Kägi, D., B. Ledermann, K. Bürki, P. Seiler, B. Odermatt, K.J. Olsen, E.R. Podack, R.M. Zinkernagel, and H. Hengartner. 1994. Cytotoxicity mediated by T cells and natural killer cells is greatly impaired in perforin-deficient mice. Nature (Lond.). 369:31-37.
3. Kägi, D., F. Vignaux, B. Ledermann, K. Bürki, V. Depraetere, S. Nagata, H. Hengartner, and P. Golstein. 1994. Fas and perform pathways as major mechanisms of T cell-mediated cytotoxicity. Science (Wash. DC). 265:528-530.
4. Lowin, B., M. Hahne, C. Mattmann, and J. Tschopp. 1994. Cytolytic T-cell cytotoxicity is mediated through perform and Fas lytic pathways. Nature (Lond.). 370:650-652.
5. Walsh, C.M., M. Matloubian, C.-C. Liu, R. Ueda, C.G. Kurahara, J.L. Christensen, M.T.F. Huang, J.D.-E. Young, R. Ahmed, and W.R. Clark. 1994. Immune function in mice lacking the perform gene. Proc. Natl. Acad. Sci. USA. 91:10854-10858.
6. Kojima, H., N. Shimohara, S. Hanaoka, Y. Someya-Shirota, Y. Takagaki, H. Ohnoa, T. Saito, T. Katayama, H. Yagita, K. Okumura, et al. 1994. Two distinct pathways of specific-killing revealed by perform mutant cytotoxic T lymphocytes. Immunity. 1:357-364.
7. Henkart, P.A. 1994. Lymphocyte-mediated cytotoxicity: two pathways and multiple effector molecules. Immunity. 1:343-346.
8. Henkart, P.A., P.J. Millard, C.W. Reynolds, and M.P. Henkart. 1984. Cytolytic activity of purified cytoplasmic granules from cytotoxic rat large granular lymphocyte tumors. J. Exp. Med. 160:75-93.
9. Podack, E.R., H. Hengartner, and M.G. Lichtenheld. 1991. A central role of perforin in cytolysis? Annu. Rev. Immunol. 9: 129-157.
10. Hayes, M.P., G.A. Berrebi, and P.A. Henkart. 1989. Induction of target cell DNA release by the cytotoxic T lymphocyte granule protease granzyme A. J. Exp. Med. 170:933-947.
11. Nakajima, H., H.L. Park, and P.A. Henkart. 1995. Synergistic roles of granzymes A and B in mediating target cell death by rat basophilic leukemia mast cell tumors also expressing cytolysin/perforin. J. Exp. Med. 181:1037-1046.
12. Shi, L., C.-M. Kam, J.C. Powers, R. Aebersold, and A.H. Greenberg. 1992. Purification of three cytotoxic lymphocyte granule serine proteases that induce apoptosis through distinct substrate and target cell interactions. J. Exp. Med. 176:1521-1529.
13. Shi, L.F., S. Mai, S. Israels, K. Browne, J.A. Trapani, and A.H. Greenberg. 1997. Granzyme B (graB) autonomously crosses the cell membrane and perform initiates apoptosis and GraB nuclear localization. J. Exp. Med. 185:855-866.
14. Boldin, M.P., T.M. Goncharov, Y.V. Goltsev, and D. Wallach. 1996. Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death. Cell. 85:803-815.
15. Darmon, A.J., D.W. Nicholson, and R.C. Bleackley. 1995. Activation of the apoptotic protease CPP32 by cytotoxic T-cell-derived granzyme B. Nature (Lond.). 377:446-448.
16. Quan, L.T., M. Tewari, K. O'Rourke, V. Dixit, S.J. Snipas, G.G. Poirier, C. Ray, D.J. Pickup, and G.S. Salvesen. 1996. Proteolytic activation of the cell death protease Yama/CPP32 by granzyme B. Proc. Natl. Acad. Sci. USA. 93:1972-1976.
17. Fernandes-Alnemri, T., R.C. Armstrong, J. Krebs, S.M. Srinivasula, L. Wang, F. Bullrich, L.C. Fritz, J.A. Trapani, K.J. Tomaselli, G. Litwack, and E.S. Alnemri. 1996. In vitro activation of Cpp32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. Proc. Natl. Acad. Sci. USA. 93:7464-7469.
18. Heusel, J.W., R.L. Wesselschmidt, S. Shresta, J.H. Russell, and T.J. Ley. 1994. Cytotoxic lymphocytes require granzyme B for the rapid induction of DNA fragmentation and apoptosis in allogeneic target cells. Cell. 76:977-987.
19. Anel, A., S. Gamen, M.A. Alava, A.M. Schmitt-Verhulst, A. Pineiro, and J. Naval. 1997. Inhibition of CPP32-like proteases prevents granzyme B- and Fas-, but not granzyme A-based cytotoxicity exerted by CTL clones. J. of Immunol. 158:1999-2006.
20. Shiver, J.W., L. Su, and P.A. Henkart. 1992. Cytotoxicity with target DNA breakdown by rat basophilic leukemia cells expressing both cytolysin and granzyme A. Cell. 71:315-322.
21. Macen, J.L., R.S. Garner, P.Y. Musy, M.A. Brooks, P.C. Turner, R.W. Moyer, G. McFadden, and R.C. Bleackley. 1996. Differential inhibition of the Fas- and granule-mediated cytolysis pathways by the orthopoxvirus cytokine response modifier A/SPI-2 and SPI-1 protein. Proc. Natl. Acad. Sci. USA. 93:9108-9113.
22. Ebnet, K., M. Hausmann, F. Lehmann-Grube, A. Müllbacher, M. Kopf, M. Lamers, and M.M. Simon. 1995. Granzyme A-deficient mice retain potent cell-mediated cytotoxicity. EMBO (Eur. Mol. Biol. Organ.) J. 14:4230-4239.
23. Müllbacher, A., K. Ebnet, R.V. Blanden, T. Stehle, C. Museteanu, and M.M. Simon. 1996. Granzyme A is essential for recovery of mice from infection with the natural cytopathic viral pathogen, ectromelia. Proc. Natl. Acad. Sci. USA. 93:5783-5787.
24. Lowin, B., F. Beermann, A. Schmidt, and J. Tschopp. 1994. A null mutation in the perforin gene impairs cytolytic T lymphocyte- and natural killer cell-mediated cytotoxicity. Proc. Natl. Acad. Sci. USA. 91:11571-11575.
25. Müllbacher, A., A.B. Hill, R.V. Blanden, W.B. Cowden, N.J. King, and R.T. Hla. 1991. Alloreactive cytotoxic T cells recognize MHC class I antigen without peptide specificity. J. Immunol. 147:1765-1772.
26. Rammensee, H.G., T. Friede, and S. Stevanoviic. 1995. MHC ligands and peptide motifs: first listing. Immunogenetics 414:178-228.
27. Flynn, K., and A. Mullbacher. 1996. Memory alloreactive cytotoxic T cells do not require costimulation for activation in vitro. Immunol. Cell Biol. 74:413-420.
28. Yap, K.L., and G.L. Ada. 1977. Cytotoxic T cells specific for influenza virus-infected target cells. Immunology 32:151-159.
29. Morelli, L., Y. Lusigman, and S. Lemieux. 1992. Heterogeneity of natural killer cell subsets in NK-1.1+ and NK-1.1-inbred mouse strains and their progeny. Cell. Immunol. 141:148-160.
30. Müllbacher, A., and N.J.C. King. 1989. Target cell lysis by natural killer cells is influenced by β2-microglobulin expression Scand.J. Immunol. 30:21-29.
31. Russell, J.H. 1983. Internal disintegration model of cytotoxic lymphocyte-induced target damage. Immunol. Rev. 72:97-118.
32. Vignaux, F., E. Vivier, B. Malissen, V. Depraetere, S. Nagata, and P. Golstein. 1995. TCR/CD3 coupling to Fas-based cytotoxicity. J. Exp. Med. 181:781-786.
33. Shresta, S., D.M. MacIvor, J.W. Heusel, J.H. Russell, and T.J. Ley. 1995. Natural killer and lymphokine-activated killer cells require granzyme B for the rapid induction of apoptosis in susceptible target cells. Proc. Natl. Acad. Sci. USA. 92: 5679-5683.
34. Simon, M.M., K. Ebnet, and M.D. Kramer. 1993. Molecular analysis and possible pleiotropic function(s) of the T cell-specific serine proteinase-1 (TSP-1). In Cytotoxic Cells: Effector Function, Generation, Methods. M. Sitkowsky and P.A. Henkart, editors. Birkhäuser, Boston. 278-294.
35. Haddad, P., D.E. Jenne, O. Kraehenbuehl, and J. Tschopp. 1993. Structure and possible functions of lymphocyte granzymes. In Cytotoxic cells: Effector Function, Generation, Methods. M.V. Sitkovsky and P.A. Henkart, editors. Birkhäuser, Boston. 251-262.
36. Blanden, R.V., B.D. Deak, and H.O. McDevitt. 1989. Strategies in virus-host interactions. In Immunology of Virus Diseases. R.V. Blanden, editor. Brolga Press, Curtin, Australia.