CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis

Journal of Cell Biology

Volumn 155, Issue 7, 2001, Pages 1147-1157

  Zeitlin, Samantha G ^a   Shelby, Richard D ^a   Sullivan, Kevin F ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Aurora B; CENP A; Cytokinesis; Midbody; PP1 1

Indexed keywords

AURORA B KINASE; CELL PROTEIN; CENTROMERE PROTEIN A; PROTEIN INCENP; PROTEIN KINASE; UNCLASSIFIED DRUG;

ARTICLE; CELL CYCLE G2 PHASE; CELL SEPARATION; CENTROMERE; CHROMOSOME ANALYSIS; CONTROLLED STUDY; CYTOKINESIS; ENZYME ACTIVITY; HUMAN; HUMAN CELL; IN VITRO STUDY; MITOSIS SPINDLE; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; REGULATORY MECHANISM;

ANIMALS; AUTOANTIGENS; BLOTTING, WESTERN; CAENORHABDITIS ELEGANS; CELL LINE; CENTROMERE; CHROMOSOMAL PROTEINS, NON-HISTONE; CYTOKINES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENT ANTIBODY TECHNIQUE; HISTONES; HUMANS; MITOSIS; MUTAGENESIS, SITE-DIRECTED; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; TRANSFECTION;

EID: 0035945340     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200108125     Document Type: Article

References (58)

1. Chromosomal passengers and the (aurora) ABCs of mitosis
2. Essential roles of Drosophila inner centromere protein (INCENP) and aurora B in histone H3 phosphorylation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation
3. INCENP binds the Aurora-related kinase AIRK2 and is required to target it to chromosomes, the central spindle and cleavage furrow
4. INCENP centromere and spindle targeting: Identification of essential conserved motifs and involvement of heterochromatin protein HP1
5. Centromeres, checkpoints and chromatid cohesion
6. Closing the cell cycle circle in yeast: G2 cyclin proteolysis initiated at mitosis persists until the activation of G1 cyclins in the next cycle
7. Differential subcellular localization of protein phosphatase-1 α, γ1, and Δ isoforms during both interphase and mitosis in mammalian cells
8. Cyclin-like accumulation and loss of the putative kinetochore motor CENP-E results from coupling continuous synthesis with specific degradation at the end of mitosis
9. Increased phosphorylation of histone H1 in mouse fibroblasts transformed with oncogenes or constitutively active mitogen-activated protein kinase kinase
10. Down-regulation of survivin by antisense oligonucleotides increases apoptosis, inhibits cytokinesis and anchorage-independent growth
11. Serine/threonine protein phosphatase type 1gamma1 is required for the completion of cytokinesis in human A549 lung carcinoma cells
12. The inner centromere protein (INCENP) antigens: Movement from inner centomere to midbody during mitosis
13. Efficient transformation of mammalian cells with constructs containing a puromycin-resistance marker
14. Mitotic histone H3 phosphorylation by the NIMA kinase in Auspergillus nidulans
15. Chromosomal passengers: Toward an integrated view of mitosis
16. Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation
17. Direct binding of CDC20 protein family members activates the anaphase-promoting complex in mitosis and G1
18. Regulation of yeast chromosome segregation by Ipl1 protein kinase and type 1 protein phosphatase
19. Type 1 protein phosphatase acts in opposition to IpL1 protein kinase in regulating yeast chromosome segregation
20. Drosophila aurora B kinase is required for histone H3 phosphorylation and condensin recruitment during chromosome condensation and to organize the central spindle during cytokinesis
21. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters
22. Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase
23. Identification and characterization of columbid annexin Icp37. Insights into the evolution of annexin 1 phosphorylation sites
24. Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation
25. Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice
26. Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes
27. Convergent and divergent evolution of regulatory sites in eukaryotic phosphorylases
28. Active and inactive protein kinases: Structural basis for regulation
29. Incenp and an aurora-like kinase form a complex essential for chromosome segregation and efficient completion of cytokinesis
30. Identification of human Rap1: Implications for telomere evolution
31. Mitotic regulation of microtubule cross-linking activity of CENP-E kinetochore protein
32. A dominant mutant of inner centromere protein (INCENP), a chromosomal protein, disrupts prometaphase congression and cytokinesis
33. Isolation and initial characterization of the mammalian midbody
34. Myosin II regulatory light chain as a novel substrate for AIM-1, an aurora/Ipl1-related kinase from rat
35. Chromatin-associated protein phosphatase 1 regulates aurora-B and histone H3 phosphorylation
36. Regulation of apoptosis at cell division by p34cdc2 phosphorylation of survivin
37. Cyclin DI-mediated inhibition of repair and replicative DNA synthesis in human fibroblasts
38. A human condensin complex contianing hCAP-C-hCAP-E and CNAP1, a homolog of Xenopus XCAP-D2, colocalizes with phosphorylated histone H3 during the early stage of mitotic chromosome condensation
39. AIR-2: An Autora/Ipl1-related protein kinase associated with chromosomes and midbody microtubules is required for polar body extrusion and cytokinesis in Caenorhabditis elegans embryos
40. The aurora-related kinase AIR-2 recruits ZEN-4/CeMKLP1 to the mitotic spindle at metaphase and is required for cytokinesis
41. Assembly of CENP-A into centromeric chromatin requires a cooperative array of nucleosomal DNA contact sites
42. Human survivin is a kinetochore-associated passenger protein
43. Selective increase in expression of isoform PP1 gamma 1 of type-1 protein phosphatase in chondrosarcoma cells
44. Enhanced expression of catalytic subunit isoform PP1 γy1 phosphatase type 1 associated with malignancy of osteogenic tumor
45. Enhanced expression of PP1 γ1, a catalytic subunit isoform of protein phosphatase type 1, in invasive ductal carcinoma of the breast
46. Nonperiodic activity of the human anaphase-promoting complex-Cdh1 ubiquitin ligase results in continuous DNA synthesis uncoupled from mitosis
47. The survivin-like C elegans BIR-1 protein acts with the Aurora-like kinase AIR-2 to affect chromosomes and the spindle midzone
48. A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosis
49. Microtubules, membranes and cytokinesis
50. Human CENP-A contains a histone H3-related histone fold domain that is required for targeting to the centromere
51. Multinuclearity and increased ploidy caused by overexpression of the aurora- and Ipl1-like midbody-associated protein mitotic kinase in human cancer cells
52. AIM-1: A mammalian midbody-associated protein required for cytokinesis
53. Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype
54. Immunolocalization of CENP-A suggests a distinct nucleosome structure at the inner kinetochore plate of active centromeres
55. Mad2 binding by phospborylated kinetochores links error detection and checkpoint action in mitosis
56. Phosphorylation of histone H3 is required for proper chromosome condensation and segregation
57. Enhanced expression of catalytic subunit isoform PP1 γ 1 of protein phosphatase type 1 in malignant fibrous histiocytoma
58. Differential regulation of CENP-A and histone H3 phosphorylation in G2/M