Peroxidase-labelling of human serum transferrin by conjugation to oligosaccharide moieties

Clinica Chimica Acta

Volumn 274, Issue 2, 1998, Pages 189-197

  D'Alessandro, Anna M ^a   D'Andrea, Gabriele ^a   Oratore, Arduino ^a  

^a UNIVERSITY OF L'AQUILA   (Italy)

Author keywords

Horseradish peroxidase; Human serum transferrin; Non isotopic probe; Sodium periodate; Transferrin receptor; Western blotting

Indexed keywords

HORSERADISH PEROXIDASE; OLIGOSACCHARIDE; PERIODATE SODIUM; PEROXIDASE; TRANSFERRIN;

ARTICLE; CONJUGATION; DERIVATIZATION; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNOBLOTTING; OXIDATION; PRIORITY JOURNAL;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOENZYME TECHNIQUES; OLIGOSACCHARIDES; PEROXIDASE; RECEPTORS, TRANSFERRIN; TRANSFERRIN;

ARMORACIA RUSTICANA;

EID: 0032557936     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-8981(98)00061-8     Document Type: Article

References (22)

1. [1] Ghose TI, Blair AH, Kulkarni PN. Preparation of antibody-linked cytotoxic agents. In: Langone JJ, Van Vunakis H, editors. Methods in enzymology, vol. 93, New York: Academic Press, 1983:280-333.
2. [2] Wilchek M, Bayer EA. The avidin-biotin complex in immunology. Immunol Today 1984;5:39-43.
3. [3] Wilchek M, Bayer EA. Hydrazide reagents for labeling glycoproteins. In: Jacoby WB, Wilchek M, editors. Methods in enzymology, vol. 138, New York: Academic Press, 1986:429-42.
4. [4] Hams DC, Aisen P. Physical biochemistry of the transferrins. In: Loehr TM, editor. Iron carriers and iron protein. New York: VCH Publisher Inc., 1989:239-351.
5. [5] Aisen P. Physical biochemistry of the transferrins: update, 1984-1988. In: Loehr TM, editor. Iron carriers and iron protein. New York: VCH Publisher Inc., 1989:353-71.
6. [6] Trowbridge IS, Omary MB. Human cell surface glycoprotein related to cell proliferation is the receptor for transferrin. Proc Natl Acad Sci USA 1981;78:3039-43.
7. [7] Willingham MC, Hanover JA, Dickson RB, Pastan I. Morphologic characterization of the pathway of transferrin endocytosis and recycling in human KB cells. Proc Natl Acad Sci USA 1984;81:175-9.
8. [8] Klausner RD, Harford J, van Renswoude J. Rapid internalization of the transferrin receptor in K562 cells is triggered by ligand binding or treatment with phorbol ester. Proc Natl Acad Sci USA 1984;81:3005-9.
9. [9] Bérczi A, Ruthner M, Szüts V, Fritzer M, Schweinzer E, Goldenberg H. Influence of conjugation of doxorubicin to transferrin on the iron uptake by K562 cells via receptor-mediated endocytosis. Eur J Biochem 1993;213:427-36.
10. [10] Avrameas S. Coupling of enzymes to proteins with glutaraldehyde. Use of conjugates for the detection of antigens and antibodies. Immunochemistry 1969;6:43-52.
11. [11] O'Shannessy DJ, Quarles RH. Specific conjugation reactions of the oligosaccharide moieties of immunoglobulins. J Appl Biochem 1985;7:347-55.
12. [12] O'Shannessy DJ, Quarles RH. Labeling of the oligosaccharide moieties of immunoglobulins. J Immunol Meth 1987;99:153-61.
13. [13] Anderson GJ, Mackerras A, Powell LW, Alliday JW. Improved purification of the human placental transferrin receptor and a novel immunoradiometric assay for receptor protein. Biochim Biophys Acta 1986;884:225-33.
14. [14] Laemmli UK. Cleavage of structural proteins during the assembly of head of bacteriophage T4. Nature 1970;27:680-5.
15. [15] Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 1979;76:4350-4.
16. [16] Hopkins CR, Trowbridge IS. Internalization and processing of transferrin and the transferrin receptor in human carcinoma A431 cells. J Cell Biol 1983;97:508-21.
17. [17] Bottomley SS, Wolfe LC, Bridges KR. Iron metabolism in K562 erythroleukemic cells. J Biol Chem 1985;260:6811-5.
18. [18] Stein BS, Sussman HH. Demonstration of two distinct transferrin receptor recycling pathways and transferrin-independent receptor internalization in K562 cells. J Biol Chem 1986;261:10319-31.
19. [19] Spik G, Mazurier J. Comparative structural and conformational studies of polypeptide chain, carbohydrate moiety and binding sites of human serotransferrin and lactofemn. In: Brown EB, Aisen P, Fielding J, Crichton RR, editors. Proteins of iron metabolism, New York: Grune and Stratton, 1977:143-51.
20. [20] van Noort WL, de Jong G, van Eijk HG. Purification of isotransferrins by concanavalin A sepharose chromatography and preparative isoelectric focusing. Eur J Clin Chem Clin Biochem 1994;32:885-92.
21. [21] Mason AB, Miller MK, Funk WD, Banfield DK, Savage KJ, Oliver RWA, Green BN, MacGillivray RTA, Woodworth RC. Expression of glycosylated and nonglycosylated human transferrin in mammalian cells. Characterization of the recombinant proteins with comparison to three commercially available transferrins. Biochemistry 1993;32:5472-9.
22. [22] Hoefkens P, Huijskes-Heins MIE, de Jeu-Jaspars CMH, van Noort WL, van Eijk HG. Influence of transferrin glycans on receptor binding and iron-donation. Glicoconjugate J 1997;14:289-95.