The crystal structure of β-Ketoacyl-acyl carrier protein synthase II from Synechocystis sp. at 1.54 Å resolution and its relationship to other condensing enzymes

Journal of Molecular Biology

Volumn 305, Issue 3, 2001, Pages 491-503

  Moche, Martin ^a   Dehesh, Katayoon ^b   Edwards, Patricia ^b   Lindqvist, Ylva ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b Calgene Inc   (United States)

Author keywords

3D structure comparison; Condensing enzyme; Fatty acid biosynthesis; Polyketide biosynthesis; Thiolase

Indexed keywords

3 OXOACYL ACYL CARRIER PROTEIN SYNTHASE; COENZYME A; FATTY ACID;

ARTICLE; CANCER; CATALYSIS; CHEMICAL BOND; CRYSTAL STRUCTURE; CYANOBACTERIUM; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; FATTY ACID SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; TUBERCULOSIS;

EID: 0035910286     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4272     Document Type: Article

References (50)

1. Regulation of fatty acid biosynthesis in Escherichia coli
2. Plant lipids: Metabolism, mutants and membranes
3. Tolerance and specificity of polyketide synthases
4. Fatty acid synthase, a proficient multifuctional enzyme
5. Binding site of cerulenin in fatty acid synthetase
6. β-Ketoacyl-ACP synthase I of Escherichia coli: Nucleotide sequence of the fabB gene and identification of the cerulenin binding residue
7. Inhibition of fatty acid synthetases by the antibiotic cerulenin
8. Purification and characterization of 3-ketoacyl-acyl carrier protein synthase III from spinach. A condensing enzyme utilizing acetyl-coenzyme A to initiate fatty acid synthesis
9. Overproduction of cis-vaccenic acid and altered temperature control of fatty acid synthesis in a mutant of Escherichia coli
10. Cloning of the fabF gene in an expression vector and in vitro characterization of recombinant fabF and fabB encoded enzymes from Escherichia coli
11. Structural, enzymatic, and genetic studies of beta-ketoacyl-acyl carrier protein synthases I and II of Escherichia coli
12. Escherichia coli as a model for the regulation of dissociable (type II) fatty acid biosynthesis
13. Isolation and characterization of the beta-ketoacyl-acyl carrier protein synthase III gene (fabH) from Escherichia coli K-12
14. Acetoacetyl-acyl carrier protein synthase, a potential regulator of fatty acid biosynthesis in bacteria
15. A cerulenin insensitive short chain 3-ketoacyl-acyl carrier protein synthase in Spinachia oleracea leaves
16. Beta-ketoacyl-acyl carrier protein synthase II of Escherichia coli. Evidence for function in the thermal regulation of fatty acid synthesis
17. Effects of the temperature range and the lack of beta-ketoacyl acyl-carrier protein synthase II on fatty acid synthesis in Escherichia coli K12 after shifts in temperature
18. Crystal structure of beta-ketoacyl-acyl carrier protein synthase II from E. coli reveals the molecular architecture of condensing enzymes
19. Structure of the complex between the antibiotic cerulenin and its target, beta-ketoacyl-acyl carrier protein synthase
20. The X-ray crystal structure of beta-ketoacyl [acyl carrier protein] synthase I
21. The 1.8 Å crystal structure and active-site architecture of beta-ketoacyl-acyl carrier protein synthase III (FabH) from Escherichia coli
22. Crystal structure of beta-ketoacyl-acyl carrier protein synthase III. A key condensing enzyme in bacterial fatty acid biosynthesis
23. Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis
24. A biosynthetic thiolase in complex with a reaction intermediate: The crystal structure provides new insights into the catalytic mechanism
25. The 2.8 Å crystal structure of peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: A five-layered alpha beta alpha beta alpha structure constructed from two core domains of identical topology
26. The 1.8 Å crystal structure of the dimeric peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: Implications for substrate binding and reaction mechanism
27. Re-engineering ketoacyl synthase specificity
28. Conversion of a beta-ketoacyl synthase to a malonyl decarboxylase by replacement of the active-site cysteine with glutamine
29. Identification and characterization of the niddamycin polyketide synthase genes from Streptomyces caelestis
30. Production of a novel polyketide through the construction of a hybrid polyketide synthase
31. A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: Architecture of metabolic diversity
32. Free R-value: A novel statistical quantity for assessing the accuracy of a crystal structure
33. Improved coefficients for map calculation using partial structures with errors
34. Improved methods for building protein models into electron density maps and the location of errors in these models
35. Crystallography and NMR system: A new software suite for macromolecular structure determination
36. TOP: A new method for protein structure comparisions and similarity searches
37. KAS IV: A 3-ketoacyl-ACP synthase from Cuphea sp. is a medium chain specific condensing enzyme
38. Conserved residues in condensing enzyme domains of fatty acid synthase and related sequences
39. Mapping of functional interactions between domains of the animal fatty acid synthase by mutant complementation in vitro
40. Defining the functions of highly conserved residues in β-ketoacyl-ACP synthases
41. Oscillation data reduction program
42. PATTERN: A precession simulating program for displaying reflection data of reciprocal space
43. The CCP4 suite: Programs for protein crystallography
44. AMoRe: An automated package for molecular replacement
45. Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions
46. Efficient rebuilding of protein structures
47. PROCHECK: A program to check the quality of protein structures
48. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
49. Raster3D version 2.0: A program for photorealistic molecular graphics
50. An extensively modified version of MolScript that includes greatly enhanced coloring capabilities