Re-engineering ketoacyl synthase specificity

Structure

Volumn 8, Issue 6, 2000, Pages 565-566

  Val, D ^a   Banu, G ^b   Seshadri, K ^b   Lindqvist, Y ^c   Dehesh, K ^a  

^a Calgene LLC   (United States)

^b TIFR IISc Campus   (India)

^c KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

3 OXOACYL ACYL CARRIER PROTEIN SYNTHASE;

ARTICLE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; GENETIC ENGINEERING; PRIORITY JOURNAL;

EID: 0034659841     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(00)00146-5     Document Type: Article

References (8)

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2. Pizer E.S.et al. Fatty acid synthase (FAS): a target for cytotoxin antimetabolites in HL60 promyelocytic leukemia cells. Cancer Res. 56:1996;2745-2747.
3. Khisimuzi M.et al. Inhibition of a mycobacterium tuberculosis β-ketoacyl ACP synthase by isoniazid. Science. 280:1998;1607-1610.
4. Magnuson K., Jackowski S., Rock C.O., Cronan J.E. Regulation of fatty acid biosynthesis in Escherichia coli. Microbiol. Rev. 57:1994;522-542.
5. Edwards P., Sabo-Nelsen J., Metz J., Dehesh K. Cloning of the fabF gene in an expression vector and in vitro characterisation of recombinant fabF and fabB encoded enzymes from Escherichia coli. FEBS Lett. 402:1997;62-66.
6. Huang W.et al. Crystal structure of β-ketoacyl-acyl carrier protein synthase II from E. coli reveals the molecular architecture of condensing enzymes. EMBO J. 17:1998;1183-1191.
7. Moche M., Schneider G., Edwards P., Dehesh K., Lindqvist Y.J. Structure of the complex between the antibiotic cerulenin and its target β-ketoacyl-acyl carrier protein synthase. J. Biol. Chem. 274:1999;6031-6034.
8. Insight II, Release 97.5. (1998). Molecular Simulations Inc., San Diego, USA.