Identification and purification of two distinct complexes containing the five RAD51 paralogs

Genes and Development

Volumn 15, Issue 24, 2001, Pages 3296-3307

  Masson, Jean Yves ^a   Tarsounas, Madalena C ^a   Stasiak, Alicja Z ^b   Stasiak, Andrzej ^b   Shah, Rajvee ^a   McIlwraith, Michael J ^a   Benson, Fiona E ^c   West, Stephen C ^a  

^a IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

^b UNIVERSITY OF LAUSANNE   (Switzerland)

^c LANCASTER UNIVERSITY   (United Kingdom)

Author keywords

DNA repair; Genomic instability; Recombination

Indexed keywords

PROTEIN RAD51B; PROTEIN RAD51C; PROTEIN RAD51D; PROTEIN XRCC2; PROTEIN XRCC3; RAD51 PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; CROSS LINKING; DNA REPAIR; EXPRESSION VECTOR; GENETIC STABILITY; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE;

ADENOSINE TRIPHOSPHATASES; BACULOVIRIDAE; CHROMATOGRAPHY, GEL; DNA REPAIR; DNA, SINGLE-STRANDED; DNA-BINDING PROTEINS; HUMANS; MALE; MICROSCOPY, ELECTRON; PRECIPITIN TESTS; PROTEIN BINDING; PROTEIN ISOFORMS; RAD51 RECOMBINASE; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; TESTIS;

VECTORS;

EID: 0035893365     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.947001     Document Type: Article

References (64)

1. Identification of a novel human RAD51 homolog, RAD51B
2. The human RAD51 protein: Polarity of strand transfer and stimulation by hRP-A
3. Heteroduplex formation by human RAD51 protein: Effects of DNA end-structure, hRP-A and hRAD52
4. Human RAD51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro
5. Purification of human RAD51 protein by selective spermidine precipitation
6. Purification and characterisation of the human RAD51 protein, an analogue of E. coli RecA
7. Synergistic actions of RAD51 and RAD52 in genetic recombination and DNA repair
8. Initiation of DNA interstrand cross-link repair in humans: The nucleotide excision repair system makes dual incisions 5′ to the cross-linked base and removes a 22 nucleotide long to 28 nucleotide long damage-free strand
9. XRCC3 is required for assembly of RAD51 complexes in vivo
10. The RAD51 family member, RAD51L3, is a DNA-stimulated ATPase that forms a complex with XRCC2
11. XRCC3 is required for efficient repair of chromosome breaks by homologous recombination
12. Isolation of novel human and mouse genes of the recA/RAD51 recombination/repair gene family
13. The XRCC2 DNA repair gene from human and mouse encodes a novel member of the recA/RAD51 family
14. The importance of repairing stalled replication forks
15. The XRCC2 and XRCC3 repair genes are required for chromosome stability in mammalian cells
16. Role of BRCA2 in control of the RAD51 recombination and DNA repair protein
17. XRCC2 is required for genetic stability, embryonic neurogenesis and viability in mice
18. Defining the roles of nucleotide excision repair and recombination in the repair of DNA interstrand cross-links in mammalian cells
19. Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes
20. Homologous and non-homologous recombination differentially affect DNA damage repair in mice
21. RAD52 partially substitutes for the RAD51 paralog XRCC3 in maintaining chromosomal integrity in vertebrate cells
22. A Chinese hamster ovary cell line hypersensitive to ionizing radiation and deficient in repair replication
23. Assembly of RecA-like recombinases: Distinct roles from mediator proteins in mitosis and meiosis
24. Complex formation in yeast double-strand break repair: Participation of Rad51, Rad52, Rad55, and Rad57 proteins
25. Repair of interstrand cross-links in DNA of Saccharomyces cerevisiae requires two systems for DNA repair: The RAD3 system and the RAD51 system
26. Mammalian XRCC2 promotes the repair of DNA double-strand breaks by homologous recombination
27. Isolation and crosssensitivity of X-ray-sensitive mutants of V79-4 hamster cells
28. Homologous-pairing activity of the human DNA-repair proteins XRCC3-RAD51C
29. Homology-directed repair is a major double-strand break repair pathway in mammalian cells
30. XRCC2 and XRCC3, new human RAD51-family members, promote chromosome stability and protect against DNA cross-links and other damages
31. Complex formation by the human RAD51C and XRCC3 recombination repair proteins
32. Rad54 protein is targeted to pairing loci by the Rad51 nucleoprotein filament
33. Reconstitution of the strand invasion step of double-strand break repair using human RAD51, RAD52 and RPA proteins
34. Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein-A
35. Similarity of the yeast Rad51 filament to the bacterial RecA filament
36. XRCC2 is a nuclear RAD51-like protein required for damage-dependent RAD51 focus formation without need for ATP hydrolysis
37. Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins
38. Yeast Rad54 promotes Rad51-dependent homologous DNA pairing via ATP hydrolysis-driven change in DNA double helix conformation
39. XRCC3 promotes homology-directed repair of DNA damage in mammalian cells
40. Mid-gestation lethality in mice deficient for the recA-related gene, RAD51D/RAD51L3
41. Identification, characterization, and genetic mapping of RAD51D, a new mouse and human RAD51/recA-related gene
42. Isolation of human and mouse genes based on homology to REC2, a recombinational repair gene from the fungus Ustilago maydis
43. Evidence for simultaneous protein interactions between human RAD51 paralogs
44. Stimulation by Rad52 of yeast Rad51-mediated recombination
45. (-/-) background
46. Basis for avid homologous DNA strand exchange by human RAD51 and RPA
47. In vitro repair of psoralen-DNA cross-links by RecA, UvrABC, and the 5′-exonuclease of DNA polymerase I
48. Rad54 protein stimulates heteroduplex DNA formation in the synaptic phase of DNA strand exchange via specific interactions with the presynaptic Rad51 nucleoprotein filament
49. Homologous DNA recombination in vertebrate cells
50. Structural relationship of bacterial RecA proteins to recombination proteins from bacteriophage T4 and yeast
51. Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast Rad51 protein
52. Function of yeast Rad52 protein as a mediator between replication protein-A and the Rad51 recombinase
53. Yeast Rad55 and Rad57 proteins form a heterodimer that functions with replication protein-A to promote DNA strand exchange by Rad51 recombinase
54. DNA strand exchange mediated by a Rad51-ssDNA nucleoprotein filament with polarity opposite to that of RecA
55. Homologous recombination and non-homologous end-joining pathways of DNA double-strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells
56. The RAD51 paralog RAD51B promotes homologous recombinational repair
57. Chromosome instability and defective recombinational repair in knockout mutants of the five RAD51 paralogs
58. The XRCC2 DNA repair gene: Identification of a positional candidate
59. Correction of chromosomal instability and sensitivity to diverse mutagens by a cloned cDNA of the XRCC3 DNA repair gene
60. A surfeit of RAD51-like genes?
61. A recombination repair gene of Schizosaccharomyces pombe, rhp57, is a functional homolog of the Saccharomyces cerevisiae RAD57 gene and is phylogenetically related to the human XRCC3 gene
62. Chromosomal stability and the DNA double-stranded break connection
63. Nucleotide excision repair in Escherichia coli
64. Distantly related sequences in the α- and β-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold