Purification of human Rad51 protein by selective spermidine precipitation

Mutation Research - DNA Repair

Volumn 384, Issue 2, 1997, Pages 65-72

  Baumann, Peter ^a   Benson, Fiona E ^a   Hajibagheri, Nasser ^a   West, Stephen C ^a  

^a IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

Author keywords

DNA repair; Homologous pairing; Rccombination; RecA

Indexed keywords

BACTERIAL PROTEIN; RECA PROTEIN; SPERMIDINE;

ARTICLE; COLUMN CHROMATOGRAPHY; DNA REPAIR; ELECTRON MICROSCOPY; ESCHERICHIA COLI; GEL ELECTROPHORESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION;

CHROMATOGRAPHY, LIQUID; CRYSTALLIZATION; DNA-BINDING PROTEINS; ESCHERICHIA COLI; HUMANS; MICROSCOPY, ELECTRON; PRECIPITATION; RAD51 RECOMBINASE; RECOMBINANT PROTEINS; SPERMIDINE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA; MUS MUSCULUS;

EID: 0030811492     PISSN: 09218777     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0921-8777(97)00028-1     Document Type: Article

References (40)

1. A.J. Clark, A.D. Margulies, Isolation and characterization of recombination deficient mutants of Escherichia coli K12., Proc. Natl. Acad. Sci. USA 53 (1965) 451-459.
2. A.I. Roca, M.M. Cox, RecA protein: structure, function, and role in recombinational DNA repair, Prog. Nucl. Acid Res. Mol. Biol. 56 (1997) 129-222.
3. S.C. Kowalczykowski, D.A. Dixon, A.K. Eggleston, S.D. Lauder, W.M. Rehrauer, Biochemistry of homologous recombination in Escherichia coli, Microbiol. Rev. 58 (1994) 401-465.
4. S.C. Kowalczykowski, A.K. Eggleston, Homologous pairing and DNA strand exchange proteins, Ann. Rev. Biochem. 63 (1994) 991-1043.
5. R.D. Camerini-Otero, P. Hsieh, Homologous recombination proteins in prokaryotes and eukaryotes, Annu. Rev. Genet. 29 (1995) 509-552.
6. A. Stasiak, E.H. Egelman, Visualization of recombination reactions, in: R. Kucherlapati, G.R. Smith (Eds.), Genetic Recombination, American Society for Microbiology, Washington, 1988, pp. 265-308.
7. P. Howard-Flanders, S.C. West, A.J. Stasiak, Role of RecA spiral filaments in genetic recombination, Nature 309 (1984) 215-220.
8. A. Stasiak, A.Z. Stasiak, T. Koller, Visualization of RecA-DNA complexes involved in consecutive stages of an in vitro strand exchange reaction, Cold Spring Harb. Symp. Quant. Biol. 49 (1984) 561-570.
9. A. Aboussekhra, R. Chanet, A. Adjiri, F. Fabre, Semidominant suppressors of srs2 helicase mutations of Saccharomyces cerevisiae map in the RAD51 gene, whose sequence predicts a protein with similarities to prokaryotic RecA proteins, Mol. Cell. Biol. 12 (1992) 3224-3234.
10. G. Basile, M. Aker, R.K. Mortimer, Nucleotide-sequence and transcriptional regulation of the yeast recombinational repair gene RAD51, Mol. Cell. Biol. 12 (1992) 3235-3246.
11. A. Shinohara, H. Ogawa, T. Ogawa, Rad51 protein involved in repair and recombination in Saccharomyces cerevisiae is a RecA-like protein, Cell 69 (1992) 457-470.
12. T. Ogawa, X. Yu, A. Shinohara, E.H. Egelman, Similarity of the yeast Rad51 filament to the bacterial RecA filament, Science 259 (1993) 1896-1899.
13. P. Sung, Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast Rad51 protein, Science 265 (1994) 1241-1243.
14. P. Sung, D.L. Robberson, DNA strand exchange mediated by a Rad51-ssDNA nucleoprotein filament with polarity opposite to that of RecA, Cell 82 (1995) 453-461.
15. K. Maeshima, K. Morimatsu, A. Shinohara, T. Horii, RAD51 homologs in Xenopus laevis: Two distinct genes are highly expressed in ovary and testis, Gene 160 (1995) 195-200.
16. K. Maeshima, K. Morimatsu, T. Horii, Purification and characterization of XRad51.1 protein, Xenopus RAD51 homologue: recombinant XRad51.1 promotes strand exchange reaction, Genes Cells 1 (1996) 1057-1068.
17. T. Morita, Y. Yoshimura, A. Yamamoto, K. Murata, M. Mori, H. Yamamoto, A. Matsushiro, A mouse homolog of the Escherichia coli recA and Saccharomyces cerevisiae RAD51 genes, Proc. Natl. Acad. Sci. USA 90 (1993) 6577-6580.
18. A. Shinohara, H. Ogawa, Y. Matsuda, N. Ushio, K. Ikeo, T. Ogawa, Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA, Nature Genet. 4 (1993) 239-243.
19. Y. Yoshimura, T. Morita, A. Yamamoto, A. Matsushiro, Cloning and sequence of the human recA-like gene cDNA, Nucl. Acids Res. 21 (1993) 1665.
20. S.J. Sandler, L.H. Satin, H.S. Samra, A.J. Clark, recA-like genes from three archaean species with putative protein products similar to Rad51 and Dmcl proteins of the yeast Saccharomyces cerevisiae, Nucl. Acids Res. 24 (1996) 2125-2132.
21. F.E. Benson, A. Stasiak, S.C. West, Purification and characterisation of the human Rad51 protein, an analogue of E. coli RecA, EMBO J. 13 (1994) 5764-5771.
22. P. Baumann, F.E. Benson, S.C. West, Human Rad51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro, Cell 87 (1996) 757-766.
23. R.C. Gupta, L.R. Bazemore, E.I. Golub, C.M. Radding, Activities of human recombination protein Rad51, Proc. Natl. Acad. Sci. USA 94 (1997) 463-468.
24. O. Bezzubova, A. Shinohara, R.G. Mueller, H. Ogawa, J.M. Buerstedde, A chicken RAD51 homologue is expressed at high levels in lymphoid and reproductive organs, Nucl. Acids Res. 21 (1993) 1577-1580.
25. T. Haaf, E.I. Golub, G. Reddy, C.M. Radding, D.C. Ward, Nuclear foci of mammalian Rad51 recombination protein in somatic cells after DNA damage and its localization in synaptonemal complexes, Proc. Natl. Acad. Sci. USA 92 (1995) 2298-2302.
26. M.J. Li, M.C. Peakman, E.I. Golub, G. Reddy, D.C. Ward, C.M. Radding, N. Maizels, Rad51 expression and localization in B cells carrying out class switching, Proc. Natl. Acad. Sci. USA 93 (1996) 10222-10227.
27. A. Yamamoto, T. Taki, H. Yagi, T. Habu, K. Yoshida, Y. Yoshimura, K. Yamamoto, A. Matsushiro, Y. Nishimune, T. Morita, Cell-cycle dependent expression of the mouse RAD51 gene in proliferating cells, Mol. Gen. Genet. 251 (1996) 1-12.
28. T. Tsuzuki, Y. Fujii, K. Sakuma, Y. Tominaga, K. Nakao, M. Sekiguchi, A. Matsushiro, Y. Yoshimura, T. Morita, Targeted disruption of the RAD51 gene leads to lethality in embryonic mice, Proc. Natl. Acad. Sci. USA 93 (1996) 6236-6240.
29. H.W. Stürzbecher, B. Donzelmann, W. Henning, U. Knippschild, S. Buchhop, p53 is linked directly to homologous recombination processes via Rad51/RecA protein interaction, EMBO J. 15 (1996) 1992-2002.
30. D.S. Lim, P. Hasty, A mutation in mouse RAD51 results in an early embryonic lethal that is suppressed by a mutation in p53, Mol. Cell. Biol. 16 (1996) 7133-7143.
31. R. Scully, J. Chen, A. Plug, Y. Xiao, D. Weaver, J. Feunteun, T. Ashley, D.M. Livingston, Association of Brcal with Rad51 in mitotic and meiotic cells, Cell 88 (1997) 265-275.
32. S.K. Sharan, M. Morimatsu, U. Albrecht, S.S. Lim, E. Regel, C. Dinh, A. Sands, G. Eichele, P. Hasty, A. Bradley, Embryonic lethality and radiation hypersensitivity mediated by Rad51 in mice lacking Brca2, Nature 386 (1997) 804-810.
33. J. Griffith, C.G. Shores, RecA protein rapidly crystallizes in the presence of spermidine: a valuable step in its purification and physical characterization, Biochemistry 24 (1985) 158-162.
34. R.G. Lloyd, C. Buckman, F.E. Benson, Genetic analysis of conjugational recombination in Escherichia coli K12 strains deficient in RecBCD enzyme, J. Gen. Microbiol. 133 (1987) 2531-2538.
35. R. Shah, R.J. Bennett, S.C. West, Activation of RuvC Holliday junction resolvase in vitro, Nucl. Acids Res. 22 (1994) 2490-2497.
36. U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227 (1970) 680-685.
37. E. Spiess, H. Zimmerman, H. Lunsdorf, Negative staining of proteins and filaments, in: Sommerville, J., Scheer, U. (Eds.), Electron Microscopy in Molecular Biology, IRL Press, Oxford, 1987, pp. 147-166.
38. D.C. Prasher, L. Conarro, S.R. Kushner, Amplification and purification of exonuclease I from Escherichia coli K12, J. Biol. Chem. 258 (1983) 6340-6343.
39. R.M. Story, I.T. Weber, T.A. Steitz, The structure of the Escherichia coli RecA protein monomer and polymer, Nature 355 (1992) 318-325.
40. X. Yu, E.H. Egelman, The RecA hexamer is a structural homologue of ring helicases, Nature Struct. Biol. 4 (1997) 101-104.