메뉴 건너뛰기




Volumn 285, Issue 5433, 1999, Pages 1576-1579

Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50

Author keywords

[No Author keywords available]

Indexed keywords

BRCA1 PROTEIN; CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR; MESSENGER RNA; NUCLEAR PROTEIN; RNA POLYMERASE II;

EID: 0001221269     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.285.5433.1576     Document Type: Article
Times cited : (145)

References (49)
  • 1
  • 2
    • 0030784958 scopus 로고    scopus 로고
    • E. Wahle and W. Keller, Trends Biochem. Sci. 21, 247 (1996); D. F. Colgan and J. L. Manley, Genes Dev. 11, 2755 (1997).
    • (1997) Genes Dev. , vol.11 , pp. 2755
    • Colgan, D.F.1    Manley, J.L.2
  • 4
    • 0031037856 scopus 로고    scopus 로고
    • S. McCracken et al., ibid. 385, 357 (1997).
    • (1997) Nature , vol.385 , pp. 357
    • McCracken, S.1
  • 6
    • 0022819015 scopus 로고
    • E. Whitelaw and N. Proudfoot, EMBO J. 5, 2915 (1986); J. Logan, E. Falck-Pedersen, J. E. Darnell, T. Shenk, Proc. Natl. Acad. Sci. U.S.A. 84, 8306 (1987);
    • (1986) EMBO J. , vol.5 , pp. 2915
    • Whitelaw, E.1    Proudfoot, N.2
  • 8
  • 12
    • 0032246188 scopus 로고    scopus 로고
    • Y. Takagaki, R. L. Seipelt, M. L. Peterson, J. L. Manley, Cell 87, 941 (1996); Y. Takagaki and J. L. Manley, Mol. Cell 2, 761 (1998).
    • (1998) Mol. Cell , vol.2 , pp. 761
    • Takagaki, Y.1    Manley, J.L.2
  • 13
    • 0024762583 scopus 로고
    • Y. Takagaki, L. C. Ryner, J. L. Manley, Genes Dev. 3, 1711 (1989); G. M. Gilmartin and J. R. Nevins, Mol. Cell. Biol. 11, 2432 (1991); K. G. K. Murthy and J. L. Manley, J. Biol. Chem. 267, 14804 (1992).
    • (1989) Genes Dev. , vol.3 , pp. 1711
    • Takagaki, Y.1    Ryner, L.C.2    Manley, J.L.3
  • 14
    • 0026353286 scopus 로고
    • Y. Takagaki, L. C. Ryner, J. L. Manley, Genes Dev. 3, 1711 (1989); G. M. Gilmartin and J. R. Nevins, Mol. Cell. Biol. 11, 2432 (1991); K. G. K. Murthy and J. L. Manley, J. Biol. Chem. 267, 14804 (1992).
    • (1991) Mol. Cell. Biol. , vol.11 , pp. 2432
    • Gilmartin, G.M.1    Nevins, J.R.2
  • 15
    • 0026694636 scopus 로고
    • Y. Takagaki, L. C. Ryner, J. L. Manley, Genes Dev. 3, 1711 (1989); G. M. Gilmartin and J. R. Nevins, Mol. Cell. Biol. 11, 2432 (1991); K. G. K. Murthy and J. L. Manley, J. Biol. Chem. 267, 14804 (1992).
    • (1992) J. Biol. Chem. , vol.267 , pp. 14804
    • Murthy, K.G.K.1    Manley, J.L.2
  • 17
    • 0026544430 scopus 로고
    • Y. Takagaki, C. C. MacDonald, T. Shenk, J. L. Manley, Proc. Natl. Acad. Sci. U.S.A. 89, 1403 (1992); C. C. MacDonald, J. Wilusz, T. Shenk, Mol. Cell. Biol. 14, 6647 (1994); K. Beyer, T. Dandekar, W. Keller J. Biol. Chem. 272, 26769 (1997); Y. Takagaki and J. L. Manley, Mol. Cell. Biol. 17, 3907 (1997).
    • (1992) Proc. Natl. Acad. Sci. U.S.A. , vol.89 , pp. 1403
    • Takagaki, Y.1    MacDonald, C.C.2    Shenk, T.3    Manley, J.L.4
  • 18
    • 0028025647 scopus 로고
    • Y. Takagaki, C. C. MacDonald, T. Shenk, J. L. Manley, Proc. Natl. Acad. Sci. U.S.A. 89, 1403 (1992); C. C. MacDonald, J. Wilusz, T. Shenk, Mol. Cell. Biol. 14, 6647 (1994); K. Beyer, T. Dandekar, W. Keller J. Biol. Chem. 272, 26769 (1997); Y. Takagaki and J. L. Manley, Mol. Cell. Biol. 17, 3907 (1997).
    • (1994) Mol. Cell. Biol. , vol.14 , pp. 6647
    • MacDonald, C.C.1    Wilusz, J.2    Shenk, T.3
  • 19
    • 0030705196 scopus 로고    scopus 로고
    • Y. Takagaki, C. C. MacDonald, T. Shenk, J. L. Manley, Proc. Natl. Acad. Sci. U.S.A. 89, 1403 (1992); C. C. MacDonald, J. Wilusz, T. Shenk, Mol. Cell. Biol. 14, 6647 (1994); K. Beyer, T. Dandekar, W. Keller J. Biol. Chem. 272, 26769 (1997); Y. Takagaki and J. L. Manley, Mol. Cell. Biol. 17, 3907 (1997).
    • (1997) J. Biol. Chem. , vol.272 , pp. 26769
    • Beyer, K.1    Dandekar, T.2    Keller, W.3
  • 20
    • 0030920331 scopus 로고    scopus 로고
    • Y. Takagaki, C. C. MacDonald, T. Shenk, J. L. Manley, Proc. Natl. Acad. Sci. U.S.A. 89, 1403 (1992); C. C. MacDonald, J. Wilusz, T. Shenk, Mol. Cell. Biol. 14, 6647 (1994); K. Beyer, T. Dandekar, W. Keller J. Biol. Chem. 272, 26769 (1997); Y. Takagaki and J. L. Manley, Mol. Cell. Biol. 17, 3907 (1997).
    • (1997) Mol. Cell. Biol. , vol.17 , pp. 3907
    • Takagaki, Y.1    Manley, J.L.2
  • 25
    • 0345396010 scopus 로고    scopus 로고
    • note
    • Yeast interaction trap selection assays were performed as in (14). Bait plasmids were constructed by insertion of polymerase chain reaction amplified fragments into LexA(202+pl). A cDNA library from human fetal brain RNA was screened. Library plasmids from positive colonies were rescued, used to transform Escherichia coli, and sequenced. Specificity was confirmed with several other LexA fusion proteins. Although LexA-CstF-50 plus BARD1-B42 again resulted in reporter gene expression, no other combination tested resulted in detectable activity (22).
  • 31
    • 0030850047 scopus 로고    scopus 로고
    • R. Scully et al., Cell 90, 425 (1997); Y. Jin et al., Proc. Natl. Acad. Sci. U.S.A. 94, 12075 (1997).
    • (1997) Cell , vol.90 , pp. 425
    • Scully, R.1
  • 35
    • 0344102294 scopus 로고    scopus 로고
    • note
    • 35S-labeled CstF-50. Conditions were as in the coimmunoprecipitation assays, except washing was with buffer B containing 300 mM NaCl.
  • 36
    • 0344533668 scopus 로고    scopus 로고
    • note
    • 4 cut and fractionated by Superose-6 chromatography (25). Selected fractions were analyzed by immunoblotting with mAbs targeted against BARD1 (EE 6) (16), CstF-64 (9), or BRCA1 (MS110) (33). NE and fractions eluting from Superose-6 were immunoprecipitated with the anti-BARD1 polyclonal antibody, or the anti-CstF64 or BRCA1 mAbs, bound to protein A-Sepharose beads. Immunoprecipitations were carried out in buffer B at 4°C for 90 min, and washing was with buffer B. Aliquots of pellets and supematants were analyzed by SDS-PAGE and immunoblotting. The anti-BARD1 and CstF-64 antibodies did not cross-react with CstF-64 and BARD1, respectively, as judged by experiments with purified (BARD1-free) CstF and recombinant CST-BARD1 (22).
  • 38
    • 0345396003 scopus 로고    scopus 로고
    • note
    • 2. NE and added proteins or antibodies were preincubated for 15 min at 30°C, after which the pre-mRNA was added and incubation continued for an additional 90 min. RNA products were isolated and fractionated on 5% polyacrylamide, 8.3 M urea gels.
  • 40
    • 0031472370 scopus 로고    scopus 로고
    • R. Scully et al., Cell 88, 265 (1997).
    • (1997) Cell , vol.88 , pp. 265
    • Scully, R.1
  • 41
    • 0026751113 scopus 로고
    • A. Shinohara, H. Ogawa, T. Ogawa, Cell 69, 457 (1992); P. Baumann, F. E. Benson, S. C. West, ibid. 87, 757 (1996).
    • (1992) Cell , vol.69 , pp. 457
    • Shinohara, A.1    Ogawa, H.2    Ogawa, T.3
  • 42
    • 0030584084 scopus 로고    scopus 로고
    • A. Shinohara, H. Ogawa, T. Ogawa, Cell 69, 457 (1992); P. Baumann, F. E. Benson, S. C. West, ibid. 87, 757 (1996).
    • (1996) Cell , vol.87 , pp. 757
    • Baumann, P.1    Benson, F.E.2    West, S.C.3
  • 43
    • 15844390393 scopus 로고    scopus 로고
    • E. Maldonado et al., Nature 381, 86 (1996).
    • (1996) Nature , vol.381 , pp. 86
    • Maldonado, E.1
  • 44
    • 0032516654 scopus 로고    scopus 로고
    • L. C. Gowen et al., Science 281, 1009 (1998).
    • (1998) Science , vol.281 , pp. 1009
    • Gowen, L.C.1
  • 46
    • 0030570004 scopus 로고    scopus 로고
    • R. Scully et al., Science 272, 123 (1996).
    • (1996) Science , vol.272 , pp. 123
    • Scully, R.1
  • 49
    • 0345396002 scopus 로고    scopus 로고
    • note
    • Supported by National Institutes of Health (NIH) grant GM28983 to J.L.M. and an NIH Fogarty Fellowship to F.E.K. We thank Y. Takagaki for CstF plasmids and antibodies; R. Baer for BARD1 and BRCA1 encoding plasmids and anti-BARD1 antibodies; J. Chen and D. Livingston for the anti-BRCA1 antibody; R. Brent for yeast two-hybrid reagents; Y. Hirose for HeLa nuclear extract; and C. Prives, R. Baer, Y. Hirose, and R. Tacke for advice and discussions.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.