The carboxyl terminus of vertebrate poly(A) polymerase interacts with U2AF 65 to couple 3'-end processing and splicing

Genes and Development

Volumn 14, Issue 4, 2000, Pages 403-413

  Vagner, Stéphan ^a,b   Vagner, Christine ^a,c   Mattaj, Iain W ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b INSERM   (France)

^c SANOFI   (France)

Author keywords

Cleavage; Poly(A) polymerase; Polyadenylation; RNA processing; Splicing; U2AF 65

Indexed keywords

HYBRID PROTEIN; MESSENGER RNA PRECURSOR; POLYNUCLEOTIDE ADENYLYLTRANSFERASE; SMALL NUCLEAR RIBONUCLEOPROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; HELA CELL; HUMAN; HUMAN CELL; INTRON; POLYADENYLATION; PRIORITY JOURNAL; PROTEIN DOMAIN; RNA CLEAVAGE; RNA PROCESSING; RNA SPLICING; SPLICEOSOME; VERTEBRATE;

VERTEBRATA;

EID: 0034008058     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (67)

1. Abovich, N. and M. Rosbash. 1997. Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals. Cell 89: 403-412.
2. Arias, J.A., S.R. Peterson, and W.S. Dynan. 1991. Promoter-dependent phosphorylation of RNA polymerase II by a template-bound kinase. Association with transcriptional initiation. J. Biol. Chem. 266: 8055-8061.
3. Arning, S., P. Grüter, G. Bilbe, and A. Kramer. 1996. Mammalian splicing factor SF1 is encoded by variant cDNAs and binds to RNA. RNA 2: 794-810.
4. Barabino, S.M.L., B.J. Blencowe, U. Ryder, B.S. Sproat, and A.I. Lamond. 1990. Targeted snRNP depletion reveals an additional role for mammalian U1 snRNP in spliceosome assembly. Cell 63: 293-302.
5. Bauren, G., S. Belikov, and L. Wieslander. 1998. Transcriptional termination in the Balbiani ring 1 gene is closely coupled to 3′-end formation and excision of the 3′-terminal intron. Genes & Dev. 12: 2759-2769.
6. Berget, S.M. 1995. Exon recognition in vertebrate splicing. J. Biol. Chem. 270: 2411-2414.
7. Berglund, J.A., K. Chua, N. Abovich, R. Reed, and M. Rosbash. 1997. The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC. Cell 89: 781-787.
8. Berglund, J.A., N. Abovich, and M. Rosbash. 1998. A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition. Genes & Dev. 12: 858-867.
9. Boelens, W.C., E.J. Jansen, W.J. van Venrooij, R. Stripecke, I.W. Mattaj, and S.I. Gunderson. 1993. The human U1 snRNP-specific U1 A protein inhibits polyadenylation of its own premRNA. Cell 72: 881-892.
10. Chiara, M.D. and R. Reed. 1995. A two-step mechanism for 5′ and 3′ splice-site pairing. Nature 375: 510-513.
11. Cho, E.J., T. Takagi, C.R. Moore, and S. Buratowski. 1997. mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain. Genes & Dev. 11: 3319-3326.
12. Colot, H.V., F. Stutz, and M. Rosbash. 1996. The yeast splicing factor Mud13p is a commitment complex component and corresponds to CBP20, the small subunit of the nuclear capbinding complex. Genes & Dev. 10: 1699-1708.
13. Cooke, C. and J.C. Alwine. 1996. The cap and the 3′ splice site similarly affect polyadenylation efficiency. Mol. Cell. Biol. 16: 2579-2584.
14. Flaherty, S.M., P. Fortes, E. Izaurralde, I.W. Mattaj, and G.M. Gilmartin. 1997. Participation of the nuclear cap binding complex in pre-mRNA 3′ processing. Proc. Natl. Acad. Sci. 94: 11893-11898.
15. Dignam, J.D., R.M. Lebovitz, and R.G. Roeder. 1983. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11: 1475-1489.
16. Fu, X.D. 1995. The superfamily of arginine/serine-rich splicing factors. RNA 1: 663-680.
17. Fu, X.D. and T. Maniatis. 1992. The 35-kDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleoprotein particles at the 3′ splice site. Proc. Natl. Acad. Sci. 89: 1725-1729.
18. Gama-Carvalho, M., R.D. Krauss, L. Chiang, J. Valcárcel, M.R. Green, and M. Carmo-Fonseca. 1997. Targeting of U2AF65 to sites of active splicing in the nucleus. J. Cell Biol. 137: 975-987.
19. Gilmartin, G.M., M.A. McDevitt, and F.R. Nevins. 1988. Multiple factors are required for specific RNA cleavage at a poly(A) addition site. Genes & Dev. 2: 578-587.
20. Gray, N.K., S. Quick, B. Goossen, A. Constable, H. Hirling, L.C. Kuhn, and M.W. Hentze. 1993. Recombinant iron-regulatory factor functions as an iron-responsive-element-binding protein, a translational repressor and an aconitase. A functional assay for translational repression and direct demonstration of the iron switch. Eur. J. Biochem. 218: 657-667.
21. Gunderson, S.I., K. Beyer, G. Martin, W. Keller, W.C. Boelens, and I.W. Mattaj. 1994. The human U1A snRNP protein regulates polyadenylation via a direct interaction with poly(A) polymerase. Cell 76: 531-541.
22. Gunderson, S.I., S. Vagner, M. Polycarpou-Schwarz, and I.W. Mattaj. 1997. Involvement of the carboxyl terminus of vertebrate poly(A) polymerase in U1A autoregulation and in the coupling of splicing and polyadenylation. Genes & Dev. 11: 761-773.
23. Gunderson, S.I., M. Polycarpou-Schwarz, and I.W. Mattaj. 1998. U1 snRNP inhibits pre-mRNA polyadenylation through a direct interaction between U1 70K and poly(A) polymerase. Mol. Cell 1: 255-264.
24. Hashimoto, C. and J.A. Steitz. 1986. A small nuclear ribonucleoprotein associates with the AAUAAA polyadenylation signal in vitro. Cell 45: 581-591.
25. Hentze, M.W. and L.C. Kuhn. 1996. Molecular control of vertebrate iron metabolism: mRNA-based regulatory circuits operated by iron, nitric oxide, and oxidative stress. Proc. Natl. Acad. Sci. 93: 8175-8182.
26. Hentze, M.W., S.W. Caughman, T.A. Rouault, J.G. Barriocanal, A. Dancis, J.B. Harford, and R.D. Klausner. 1987. Identification of the iron-responsive element for the translational regulation of human ferritin mRNA. Science 238: 1570-1573.
27. Hertel, K.J., K.W. Lynch, and T. Maniatis. 1997. Common themes in the function of transcription and splicing enhancers. Curr. Opin. Cell Biol. 9: 350-357.
28. Hoffman, B.E. and P.J. Grabowski. 1992. U1 snRNP targets an essential splicing factor, U2AF 65, to the 3′ splice site by a network of interactions spanning the exon. Genes & Dev. 6: 2554-2568.
29. Inoue, K., M. Ohno, H. Sakamoto, and Y. Shimura. 1989. Effect of the cap structure on pre-mRNA splicing in Xenopus oocyte nuclei. Genes & Dev. 3: 1472-1479.
30. Izaurralde, E., J. Lewis, C. McGuigan, M. Jankowska, E. Darzynkiewicz, and I.W. Mattaj. 1994. A nuclear cap binding protein complex involved in pre-mRNA splicing. Cell 78: 657-668.
31. Izaurralde, E., J. Lewis, C. Gamberi, A. Jarmolowski, C. McGuigan, and I.W. Mattaj. 1995. A cap-binding protein complex mediating U snRNA export. Nature 376: 709-712.
32. Kataoka, N., M. Ohno, K. Kangawa, Y. Tokoro, and Y. Shimura. 1994. Cloning of a complementary DNA encoding an 80 kilodalton nuclear cap binding protein. Nucleic Acids Res. 22: 3861-3865.
33. Kataoka, N., M. Ohno, I. Moda, and Y. Shimura. 1995. Identification of the factors that interact with NCBP, an 80 kDa nuclear cap binding protein. Nucleic Acids Res. 23: 3638-3641.
34. Konarska, M.M. and P.A. Sharp. 1986. Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs. Cell 46: 845-855.
35. Krämer, A., M. Quentin, and F. Mulhauser. 1998. Diverse modes of alternative splicing of human splicing factor SF1 deduced from the exon-intron structure of the gene. Gene 211: 29-37.
36. Kuersten, S., K. Lea, M. MacMorris, J. Spieth, and T. Blumenthal. 1997. Relationship between 3′-end formation and SL2-specific trans-splicing in polycistronic Caenorhabditis elegans pre-mRNA processing. RNA 3: 269-278.
37. Laybourn, P.J. and M.E. Dahmus. 1990. Phosphorylation of RNA polymerase IIA occurs subsequent to interaction with the promoter and before the initiation of transcription. J. Biol. Chem. 265: 13165-13173.
38. Lewis, J.D., E. Izaurralde, A. Jarmolowski, C. McGuigan, and I.W. Mattaj. 1996a. A nuclear cap-binding complex facilitates association of U1 snRNP with the cap-proximal 5′ splice site. Genes & Dev. 10: 1683-1698.
39. Lewis, J.D., D. Görlich, and I.W. Mattaj. 1996b. A yeast cap binding protein complex (yCBC) acts at an early step in premRNA splicing. Nucleic Acids Res. 24: 3332-3336.
40. Lou, H., Y. Yang, G.J. Cote, S.M. Berget, and R.F. Gagel. 1995. An intron enhancer containing a 5́ splice site sequence in the human calcitonin/calcitonin gene-related peptide gene. Mol. Cell Biol. 15: 7135-7142.
41. Lou, H., R.F. Gagel, and S.M. Berget. 1996. An intron enhancer recognized by splicing factors activates polyadenylation. Genes & Dev. 10: 208-219.
42. MacDonald, C.C., J. Wilusz, and T. Shenk. 1994. The 64-kilodalton subunit of the CstF polyadenylation factor binds to pre-mRNAs downstream of the cleavage site and influences cleavage site location. Mol. Cell. Biol. 14: 6647-6654.
43. Manley, J.L. and R. Tacke. 1996. SR proteins and splicing control. Genes & Dev. 10: 1569-1579.
44. McCracken, S., N. Fong, K. Yankulov, S. Ballantyne, G. Pan, J. Greenblatt, S.D. Patterson, M. Wickens, and D.L. Bentley. 1997a. The C-terminal domain of RNA polymerase II couples mRNA processing to transcription. Nature 385: 357-361.
45. McCracken, S., N. Fong, E. Rosonina, K. Yankulov, G. Brothers, D. Siderovski, A. Hessel, S. Foster, S. Shuman, and D.L. Bentley. 1997b. 5′-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II. Genes & Dev. 11: 3306-3318.
46. Michaud, S. and R. Reed. 1993. A functional association between the 5′ and 3′ splice site is established in the earliest prespliceosome complex (E) in mammals. Genes & Dev. 7: 1008-1020.
47. Moore, C.L. and P.A. Sharp. 1985. Accurate cleavage and polyadenylation of exogenous RNA substrate. Cell 41: 845-855.
48. Nesic, D. and L.E. Maquat. 1994. Upstream introns influence the efficiency of final intron removal and RNA 3′-end formation. Genes & Dev. 8: 363-375.
49. Nesic, D., J. Cheng, and L.E. Maquat. 1993. Sequences within the last intron function in RNA 3′-end formation in cultured cells. Mol. Cell. Biol. 13: 3359-3369.
50. Nesic, D., J. Zhang, and L.E. Maquat. 1995. Lack of an effect of the efficiency of RNA 3′-end formation on the efficiency of removal of either the final or the penultimate intron in intact cells. Mol. Cell Biol. 15: 488-496.
51. Neugebauer, K.M. and M.B. Roth. 1997. Transcription units as RNA processing units. Genes & Dev. 11: 3279-3285.
52. Niwa, M. and S.M. Berget. 1991. Mutation of the AAUAAA polyadenylation signal depresses in vitro splicing of proximal but not distal introns. Genes & Dev. 5: 2086-2095.
53. Niwa, M., S.D. Rose, and S.M. Berget. 1990. In vitro polyadenylation is stimulated by the presence of an upstream intron. Genes & Dev. 4: 1552-1559.
54. Niwa, M., C.C. MacDonald, and S.M. Berget. 1992. Are vertebrate exons scanned during splice-site selection? Nature 360: 277-280.
55. Rain, J.C., Z. Rafi, Z. Rhani, P. Legrain, and A. Krämer. 1998. Conservation of functional domains involved in RNA binding and protein-protein interactions in human and Saccharomyces cerevisiae pre-mRNA splicing factor SF1. RNA 4: 551-565.
56. Robberson, B.L., G.J. Cote, and S.M. Berget. 1990. Exon definition may facilitate splice site selection in RNAs with multiple exons. Mol. Cell Biol. 10: 84-94.
57. Rosbash, M. and B. Séraphin. 1991. Who's on first? The U1 snRNP-5́ splice site interaction and splicing. Trends Biodiem. Sci. 16: 187-190.
58. Ruskin, B., P.D. Zamore, and M.R. Green. 1988. A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly. Cell 52: 207-219.
59. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
60. Staknis, D. and R. Reed. 1994a. Direct interactions between pre-mRNA and six U2 small nuclear ribonucleoproteins during spliceosome assembly. Mol Cell. Biol. 14: 2994-3005.
61. _. 1994b. SR proteins promote the first specific recognition of Pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex. Mol. Cell. Biol. 14: 7670-7682.
62. Valcárcel, J. and M.R. Green. 1996. The SR protein family: Pleiotropic functions in pre-mRNA splicing. Trends Biochem. Sci. 21: 296-301.
63. van Gelder, C.W., S.I. Gunderson, E.J. Jansen, W.C. Boelens, M. Polycarpou-Schwarz, I.W. Mattaj, and W.J. van Venrooij. 1993. A complex secondary structure in U1A pre-mRNA that binds two molecules of U1 A protein is required for regulation of polyadenylation. EMBO J. 12: 5191-5200.
64. Wassarman, K.M. and J.A. Steitz. 1993. Association with terminal exons in pre-mRNAs: A new role for the U1 snRNP? Genes & Dev. 7: 647-659.
65. Xiao, S.H. and J.L. Manley. 1997. Phosphorylation of the ASF/ SF2 RS domain affects both protein-protein and protein-RNA interactions and is necessary for splicing. Genes & Dev. 11: 334-344.
66. Zamore, P. D., J.G. Patton, and M.R. Green. 1992. Cloning and domain structure of the mammalian splicing factor U2AF. Nature 355: 609-614.
67. Zuo, P. and T. Maniatis. 1996. The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing. Genes & Dev. 10: 1356-1368.