Creation and characterization of 5-fluorodeoxyuridine-resistant Arg50loop mutants of human thymidylate synthase

Cancer Research

Volumn 61, Issue 2, 2001, Pages 666-672

  Landis, Daniel M ^a   Heindel, Cory C ^a   Loeb, Lawrence A ^a  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLOXURIDINE; MUTANT PROTEIN; THYMIDYLATE SYNTHASE;

AMINO ACID SUBSTITUTION; ARTICLE; DRUG RESISTANCE; DRUG SYNTHESIS; ESCHERICHIA COLI; GENE THERAPY; HUMAN; HUMAN CELL; MUTAGENESIS; PRIORITY JOURNAL;

ANTIMETABOLITES, ANTINEOPLASTIC; ARGININE; CLONING, MOLECULAR; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG RESISTANCE; ESCHERICHIA COLI; FLOXURIDINE; GENE LIBRARY; GENETIC COMPLEMENTATION TEST; HUMANS; KINETICS; MUTAGENESIS; MUTATION; THYMIDYLATE SYNTHASE;

EID: 0035863427     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Thymineless death and genetic events in mammalian cells
2. Thymidylate synthase inhibitors
3. Atomic structure of thymidylate synthase: Target for rational drug design
4. The catalytic mechanism and structure of thymidylate synthase
5. Isolation and characterization of Thymitaq (AG337) and 5-fluoro-2-deoxyuridylate-resistant mutants of human thymidylate synthase from ethyl methanesulfonate-exposed human sarcoma HT1080 cells
6. Probing the folate-binding site of human thymidylate synthase by site-directed mutagenesis
7. Random sequence mutagenesis and resistance to 5-fluorouridine in human thymidylate synthases
8. Tolerance of 5-fluorodeoxyuridine resistant human thymidylate symhases to alterations in active site residues
9. Mechanisms of acquired resistance to thymidylate synthase inhibitors: The role of enzyme stability
10. Crystal structure of human thymidylate synthase: A structural mechanism for guiding substrates into the active site
11. Structure, multiple site binding, and segmental accommodation in thymidylate synthase on binding dUMP and an anti-folate
12. Crystal structure of Escherichia coli thymidylate synthase containing bound 5-fluoro-2′-deoxyuridylate and 10-propargyl-5,8-dideazafolate
13. Increasing DNA repair methyltransferase levels via bone marrow stem cell transduction rescues mice from the toxic effects of 1,3-bis(2-cloroethyl)-1-nitrosourea, a chemotherapeutic alkylating agent
14. Chemotherapy and chemosensitization of transgenic mice which express the human multidrug resistance gene in bone marrow: Efficacy, potency, and toxicity
15. Expression of human thymidylate synthase in Escherichia coli
16. Kinetics and mechanism of interaction of 10-propargyl-5,8-dideazafolate with thymidylate synthase
17. Direct spectrophotometric evidence for the oxidation of tetrahydrafolate during the enzymatic synthesis of thymidylate
18. Development of a trichloroacetic acid precipitation assay for covalent adducts of thymidylate synthase
19. Amino acid substitution analysis of E. coli thymidylate synthase: The study of a highly conserved region at the N-terminus
20. Plastic adaptation toward mutations in proteins: Structural comparison of thymidylate synthases
21. Functional effects of a naturally occurring amino acid substitution in human thymidylate synthase
22. Functional effects of amino acid substitutions at residue 33 of human thymidylate synthase
23. A naturally occurring tyrosine to histidine replacement at residue 33 of human thymidylate synthase confers resistance to 5-fluoro-2′-deoxyuridine in mammalian and bacterial cells
24. A naturally occurring variation in thymidylate synthase structure is associated with a reduced response to 5-fluoro-2′-deoxyuridine in a human colon tumor cell line
25. Single amino acid substitution defines a naturally occurring genetic variant of human thymidylate synthase
26. Escherichia coli thymidylate synthase: Amino acid substitutions by suppression of amber nonsense mutations
27. Water-mediated substrate/product discrimination: The product complex of thymidylate synthase at 1.83 A
28. Fluorinated pyrimidines