Interaction of Escherichia coli purine nucleoside phosphorylase (PNP) with the cationic and zwitterionic forms of the fluorescent substrate N(7)-methylguanosine

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1544, Issue 1-2, 2001, Pages 74-88

  Stoychev, Gerasim ^a   Kierdaszuk, Borys ^a   Shugar, David ^a,b  

^a UNIVERSITY OF WARSAW   (Poland)

^b INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS   (Poland)

Author keywords

Enzyme kinetics; Enzyme ligand interaction; Fluorescence quenching; pH effect; Purine nucleoside phosphorylase

Indexed keywords

7 METHYLGUANINE; 7 METHYLGUANOSINE; AMPHOLYTE; BACTERIAL ENZYME; CATION; GUANOSINE; PURINE NUCLEOSIDE PHOSPHORYLASE;

ABSORPTION; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME KINETICS; ENZYME PHOSPHORYLATION; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; FLUORESCENCE SPECTROSCOPY; LIGAND BINDING; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; REACTION ANALYSIS; STEADY STATE;

DEOXYGUANOSINE; ESCHERICHIA COLI; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; PURINE-NUCLEOSIDE PHOSPHORYLASE; SPECTROMETRY, FLUORESCENCE;

ESCHERICHIA COLI; MAMMALIA;

EID: 0035846969     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(00)00206-5     Document Type: Article

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