-
1
-
-
0019568388
-
-
L. A. Klobutcher, M. T. Swanton, P. Donini, D. M. Prescott, Proc. Natl. Acad. Sci. U.S.A. 78, 3015 (1981).
-
(1981)
Proc. Natl. Acad. Sci. U.S.A.
, vol.78
, pp. 3015
-
-
Klobutcher, L.A.1
Swanton, M.T.2
Donini, P.3
Prescott, D.M.4
-
3
-
-
0343721193
-
-
note
-
The presence of 3′ single-stranded extensions in S. pombe was verified by hybridization of native genomic DNA with a telomeric probe before and after exonuclease 1 treatment (25).
-
-
-
-
6
-
-
0030731928
-
-
W. E. Wright, V. M. Tesmer, K. E. Huffman, S. D. Levene, J. W. Shay, Genes Dev. 11, 2801 (1997).
-
(1997)
Genes Dev.
, vol.11
, pp. 2801
-
-
Wright, W.E.1
Tesmer, V.M.2
Huffman, K.E.3
Levene, S.D.4
Shay, J.W.5
-
11
-
-
0025984268
-
-
J. T. Gray, D. W. Celander, C. M. Price, T. R. Cech, Cell 67, 807 (1991).
-
(1991)
Cell
, vol.67
, pp. 807
-
-
Gray, J.T.1
Celander, D.W.2
Price, C.M.3
Cech, T.R.4
-
12
-
-
0342416022
-
-
note
-
The O. nova α subunit by itself preferentially binds the 3′ terminus of a telomeric oligonucleotide, but also binds single-stranded telomeric DNA internally (11).
-
-
-
-
14
-
-
0032431057
-
-
M. P. Horvath, V. L. Schweiker, J. M. Bevilacqua, J. A. Ruggles, S. C. Schultz, Cell 95, 963 (1998).
-
(1998)
Cell
, vol.95
, pp. 963
-
-
Horvath, M.P.1
Schweiker, V.L.2
Bevilacqua, J.M.3
Ruggles, J.A.4
Schultz, S.C.5
-
15
-
-
0027104087
-
-
W. Wang, R. Skopp, M. Scofield, C. Price, Nucleic Acids Res. 20, 6621 (1992).
-
(1992)
Nucleic Acids Res.
, vol.20
, pp. 6621
-
-
Wang, W.1
Skopp, R.2
Scofield, M.3
Price, C.4
-
16
-
-
0033553536
-
-
J. D. Griffith et al., Cell 97, 503 (1999).
-
(1999)
Cell
, vol.97
, pp. 503
-
-
Griffith, J.D.1
-
17
-
-
0343721188
-
-
note
-
+ with the kanamycin resistance gene as described (34).
-
-
-
-
21
-
-
0342850899
-
-
note
-
4 (pH 8.0), 0.6 M NaCl, 10% glycerol, 0.2% Tween-20, and 5 mM β-mercaptoethanol] containing increasing concentrations of imidazole. Pot1p eluted around 90 mM imidazote. Pot1p-containing fractions were dialyzed against T buffer [50 mM tris-HCl (pH 8.0), 10% glycerol, 0.5 mM EDTA, and 0.5 mM dithiothreitol] containing 0.2 M KCl, and Pot1p was further purified on a Q-sepharose column (Pharmacia) using a linear gradient of KCl (0.2 to 1 M). The 22-kD Pot1p fragment was found in the flowthrough, whereas Pot1p eluted around 0.5 M KCl. The protein was dialyzed against T buffer plus 0.2 M KCl and stored at -80°C.
-
-
-
-
22
-
-
0343721182
-
-
note
-
Identical results were obtained when hemagglutinin-tagged Pot1p was expressed in S. pombe and cell-free extracts were used in bandshift assays (19).
-
-
-
-
24
-
-
0028874393
-
-
J. M. Petersen et al., Science 269, 1866 (1995).
-
(1995)
Science
, vol.269
, pp. 1866
-
-
Petersen, J.M.1
-
25
-
-
0343721174
-
-
See Science Online (www.sciencemag.org/cgi/ content/full/292/5519/1171/DC1).
-
Science
-
-
-
27
-
-
0030745448
-
-
M. Meyerson et al., Cell 90, 785 (1997).
-
(1997)
Cell
, vol.90
, pp. 785
-
-
Meyerson, M.1
-
29
-
-
0343721166
-
-
note
-
Human POT1 was amplified by polymerase chain reaction from ovary cDNA and cloned into the pQE30 expression vector. The protein was purified over Ni-NTA resin under the same conditions as SpPot1p. The human protein eluted around 135 mM imidazole.
-
-
-
-
30
-
-
0032523957
-
-
S. J. Froelich-Ammon, B. A. Dickinson, J. M. Bevilacqua, S. C. Schultz, T. R. Cech, Genes Dev. 12, 1504 (1998).
-
(1998)
Genes Dev.
, vol.12
, pp. 1504
-
-
Froelich-Ammon, S.J.1
Dickinson, B.A.2
Bevilacqua, J.M.3
Schultz, S.C.4
Cech, T.R.5
-
32
-
-
0342415998
-
-
note
-
Yet another state may involve a complex with heterogeneous nuclear ribonucleoproteins, several of which have properties consistent with a role in telomere maintenance (35-38).
-
-
-
-
33
-
-
0342850880
-
-
note
-
Pot1p might also bind the displaced single strand of a D-loop within a t-loop, although the protein does have a preference for binding terminal rather than internal repeats.
-
-
-
-
35
-
-
0027263365
-
-
F. Ishikawa, M. J. Matunis, G. Dreyfuss, T. R. Cech, Mol. Cell. Biol. 13, 4301 (1993).
-
(1993)
Mol. Cell. Biol.
, vol.13
, pp. 4301
-
-
Ishikawa, F.1
Matunis, M.J.2
Dreyfuss, G.3
Cech, T.R.4
-
42
-
-
0343721140
-
-
We thank D. Lyons, G. Mellitzer, T. Nakamura, O. Peersen, V. Wood, and the members of the Cech laboratory for helpful discussions; D. King for mass spectroscopy; D. Baumann, K. Goodrich, Y. Han, and E. Podell for technical assistance; and T. Bryan, K. Friedman, and A. Zaug for critical reading of the manuscript. P.B. was supported in part by a Wellcome Prize Traveling Research Fellowship (grant 054549/Z/98/Z)
-
We thank D. Lyons, G. Mellitzer, T. Nakamura, O. Peersen, V. Wood, and the members of the Cech laboratory for helpful discussions; D. King for mass spectroscopy; D. Baumann, K. Goodrich, Y. Han, and E. Podell for technical assistance; and T. Bryan, K. Friedman, and A. Zaug for critical reading of the manuscript. P.B. was supported in part by a Wellcome Prize Traveling Research Fellowship (grant 054549/Z/98/Z).
-
-
-
|