Patterns of adaptation in a laboratory evolved thermophilic enzyme

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1549, Issue 1, 2001, Pages 1-8

  Wintrode, Patrick L ^a   Miyazaki, Kentaro ^b   Arnold, Frances H ^a  

^a California Institute of Technology   (United States)

^b NATL INST BIOSCI HUM TECHNOL   (Japan)

Author keywords

Directed evolution; Protein engineering; Psychrophile; Subtilisin; Thermophile

Indexed keywords

AMINO ACID; SUBTILISIN;

AMINO ACID SUBSTITUTION; ARTICLE; CHEMICAL MUTAGENESIS; CIRCULAR DICHROISM; COMPARATIVE STUDY; HALF LIFE TIME; HEAT SENSITIVITY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN VARIANT; TEMPERATURE SENSITIVITY;

AMINO ACIDS; CHYMOTRYPSIN; CIRCULAR DICHROISM; ENZYME STABILITY; HALF-LIFE; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; SUBTILISIN; TEMPERATURE; TRYPSIN;

EID: 0035840105     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(01)00226-6     Document Type: Article

References (28)

1. The stability of proteins in extreme environments
2. Structural differences between mesophilic, moderately thermophilic and extremely thermophilic protein subunits: Results of a comprehensive survey
3. Directed evolution of a thermostable esterase
4. Directed evolution study of temperature adaptation in a psychrophilic enzyme
5. Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution
6. Improving the catalytic activity of a thermophilic enzyme at low temperatures
7. Improving low-temperature catalysis in the hyperthermostable Pyrococcus furiosus beta-glucosidase CalB by directed evolution
8. Serial increase in the thermal stability of 3-isopropylmalate dehydrogenase from Bacillus subtilis by experimental evolution
9. Molecular evolution by staggered extension process (StEP) in vitro recombination
10. Exploring nonnatural evolutionary pathways by saturation mutagenesis: Rapid improvement of protein function
11. The high resolution x-ray crystal structure of the complex formed between subtilisin Carlsberg and eglin c, an elastase inhibitor from the leech Hirudo medicinalis. Structural analysis, subtilisin structure and interface geometry
12. Structural comparison of two serine protease-protein inhibitor complexes: Eglin c-subtilisin Carlsberg and CI-2-subtilisin novo
13. Molecular dynamics refinement of a thermitase-eglin c complex at 1.98 angstrom resolution and comparison of two crystal forms that differ in calcium content
14. Crystal structure of the alkaline proteinase savanase from Bacillus lentus at 1.4 angstrom resolution
15. The protein data bank: A computer based archival file for macromolecular structures
16. Subtilases: The superfamily of subtilisin-like serine proteases
17. Directed evolution converts subtilisin E into a functional equivalent of thermitase
18. The engineering of binding affinity at metal ion binding sites for the stabilization of proteins
19. Probing the partially folded states of proteins by limited proteolysis
20. Patterns of temperature adaptation in proteins from Methanococcus and Bacillus
21. Homology modeling of two subtilisin-like serine proteases from the hyperthermophilic archea Pyrococcus furiosus and Thermococcus stetteri
22. Protein evolution on rugged landscapes
23. Stepwise intraclonal maturation of antibody affinity through somatic hypermutation
24. Replicability and recurrence in the experimental evolution of a group I ribozyme
25. 3D structural information as a guide to protein engineering using genetic selection
26. DNA shuffling of subgenomic sequences of subtilisin
27. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures