A functional interaction of Ku with Werner exonuclease facilitates digestion of damaged DNA

Nucleic Acids Research

Volumn 29, Issue 9, 2001, Pages 1926-1934

  Orren, David K ^a,c   Machwe, Amrita ^b   Karmakar, Parimal ^b   Piotrowski, Jason ^b   Cooper, Marcus P ^b,c   Bohr, Vilhelm A ^b  

^a UNIVERSITY OF KENTUCKY   (United States)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

^c JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

8 HYDROXYGUANINE; 8 OXOADENINE; ADENINE DERIVATIVE; ADENOSINE TRIPHOSPHATASE; CATION; DIMER; DNA; EXONUCLEASE; HELICASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; WERNER SYNDROME PROTEIN; 8 HYDROXYADENINE; 8-HYDROXYADENINE; 8-HYDROXYGUANINE; ADENINE; DNA BINDING PROTEIN; DNA BINDING PROTEINS; DRUG DERIVATIVE; GUANINE; KU PROTEIN, HUMAN; NUCLEAR PROTEIN; RP A PROTEIN; RP-A PROTEIN; WRN PROTEIN, HUMAN;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; AGE; ARTICLE; CONTROLLED STUDY; DNA DAMAGE; DNA DEGRADATION; HUMAN; LABORATORY; PREMATURE AGING; PRIORITY JOURNAL; PROTEIN LOCALIZATION; WERNER SYNDROME; CELL LINE; CELL NUCLEUS; CHEMISTRY; HELA CELL; METABOLISM; PHYSIOLOGY;

ADENINE; CELL LINE; CELL NUCLEUS; DNA; DNA DAMAGE; DNA HELICASES; DNA-BINDING PROTEINS; EXONUCLEASES; GUANINE; HELA CELLS; HUMAN; NUCLEAR PROTEINS; SUPPORT, NON-U.S. GOV'T;

EID: 0035339672     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/29.9.1926     Document Type: Article

References (31)

1. Martin, G.M., Austad, S.N. and Johnson, T.E. (1996) Genetic analysis of aging: role of oxidative damage and environmental stresses. Nat. Genet., 13, 25-34.
2. Martin, G.M. (1997) The pathobiology of the Werner syndrome. FASEB J., 11, A1449.
3. Yu, C.E., Oshima, J., Fu, Y.H., Wijsman, E.M., Hisama, F., Alisch, R., Matthews, S., Nakura, J., Miki, T., Ouais, S. et al. (1996) Positional cloning of the Werner's syndrome gene. Science, 272, 258-262.
4. Gray, M.D., Shen, J.C., Kamath-Loeb, A.S., Blank, A., Sopher, B.L., Martin, G.M., Oshima, J. and Loeb, L.A. (1997) The Werner syndrome protein is a DNA helicase. Nat. Genet., 17, 100-103.
5. Huang, S., Li, B., Gray, M.D., Oshima, J., Mian, I.S. and Campisi, J. (1998) The premature ageing syndrome protein, WRN, is a 3′→5′ exonuclease. Nat. Genet., 20, 114-116.
6. Kamath-Loeb, A.S., Shen, J.C., Loeb, L.A. and Fry, M. (1998) Werner syndrome protein. II. Characterization of the integral 3′→5′ DNA exonuclease. J. Biol. Chem., 273, 34145-34150.
7. Shen, J.C., Gray, M.D., Oshima, J. and Loeb, L.A. (1998)Characterization of Werner syndrome protein DNA helicase activity: directionality, substrate dependence and stimulation by replication protein A. Nucleic Acids Res., 26, 2879-2885.
8. Orren, D.K., Brosh, R.M., Jr, Nehlin, J.O., Machwe, A., Gray, M.D. and Bohr, V.A. (1999) Enzymatic and DNA binding properties of purified WRN protein: high affinity binding to single-stranded DNA but not to DNA damage induced by 4NQO. Nucleic Acids Res., 27, 3557-3566.
9. Martin, G.M., Sprague, C.A. and Epstein, C.J. (1970) Replicative life-span of cultivated human cells. Effect of donor's age, tissue and genotype. Lab. Invest., 23, 86-92.
10. Ogburn, C.E., Oshima, J., Poot, M., Chen, R., Gollahon, K.A., Rabinovitch, P.S. and Martin, G.M. (1997) An apoptosis-inducing genotoxin differentiates heterozygotic carriers for Werner helicase mutations from wild-type and homozygous mutants. Hum. Genet., 101, 121-125.
11. Lebel, M. and Leder, P. (1998) A deletion within the murine Werner syndrome helicase induces sensitivity to inhibitors of topoisomerase and loss of cellular proliferative capacity. Proc. Natl Acad. Sci. USA. 95, 13097-13102.
12. Fukuchi, K., Martin, G.M. and Monnat, R.J. (1989) Mutator phenotype of Werner syndrome is characterized by extensive deletions. Proc. Natl Acad. Sci. USA, 86, 5893-5897.
13. Stefanini, M., Scappaticci, S., Lagomarsini, P., Borroni, G., Berardesca, E. and Nuzzo, F. (1989) Chromosome instability in lymphocytes from a patient with Werner's syndrome is not associated with DNA repair defects. Mutat. Res., 219, 179-185.
14. Bohr, V.A., Dianov, G.L., Balajee, A.S., Nehlin, J.O., Gray, M.D., Brosh, R.M., Jr, Dianova, I., Machwe, A. and Orren, D.K. (1999) DNA repair in human premature aging disorders. In Bohr, V.A., Clark, B.F. and Stevnsner, T. (eds), Molecular Biology of Aging, Alfred Benzon Symposium No. 44. Munksgaard, Copenhagen, Denmark, pp. 262-272.
15. Cooper, M.P., Machwe, A., Orren, D.K., Brosh, R.M., Ramsden, D. and Bohr, V.A. (2000) Ku complex interacts with and stimulates the Werner protein. Genes Dev., 14, 907-912.
16. Li, B. and Comai, L. (2000) Functional interaction between Ku and the Werner syndrome protein in DNA end processing. J. Biol. Chem., 275, 28342-28352.
17. Featherstone, C. and Jackson, S.P. (1999) Ku, a DNA repair protein with multiple cellular functions? Mutat. Res., 434, 3-15.
18. Vogel, H., Lim, D.S., Karsenty, G., Finegold, M. and Hasty, P. (1999) Deletion of Ku86 causes early onset of senescence in mice. Proc. Natl Acad. Sci. USA, 96, 10770-10775.
19. Gu, Y., Seidl, K.J., Rathbun, G.A., Zhu, C., Manis, J.P., van der Stoep, N., Davidson, X., Cheng, H.L., Sekiguchi, J.M., Frank, K. et al. (1997) Growth retardation and leaky SCID phenotype of Ku70-deficient mice. Immunity, 7, 653-665.
20. Nussenzweig, A., Sokol, K., Burgman, P., Li, L. and Li, G.C. (1997) Hypersensitivity of Ku80-deficient cell lines and mice to DNA damage: the effects of ionizing radiation on growth, survival and development. Proc. Natl Acad. Sci. USA, 94, 13588-13593.
21. Machwe, A., Ganunis, R., Bohr, V.A. and Orren, D.K. (2000) Selective blockage of the 3′→5′ exonuclease activity of WRN protein by certain oxidative modifications and bulky lesions in DNA. Nucleic Acids Res., 28, 2762-2770.
22. Kenny, M.K., Schlegel, U., Furneaux, H. and Hurwitz, J. (1990) The role of human single-stranded DNA binding protein and its individual subunits in simian virus 40 DNA replication. J. Biol. Chem., 265, 7693-7700.
23. Ramsden, D.A. and Gellert, M. (1998) Ku protein stimulates DNA end joining by mammalian DNA ligases: a direct role for Ku in repair of DNA double-strand breaks. EMBO J., 17, 609-614.
24. Brosh, R.M., Jr, Orren, D.K., Nehlin, J.O., Ravn, P.H., Kenny, M.K., Machwe, A. and Bohr, V.A. (1999) Functional and physical interaction between WRN helicase and human replication protein A. J. Biol. Chem., 274, 18341-18350.
25. Marciniak, R.A., Lombard, D.B., Bradley Johnson, F. and Guarente, L. (1998) Nucleolar localization of the Werner syndrome protein in human cells. Proc. Natl. Acad. Sci. USA, 95, 6686-6892.
26. Gray, M.D., Wang, L., Youssoufian, H., Martin, G.M. and Oshima, J. (1998) Werner helicase is localized to transcriptionally active nucleoli of cycling cells. Exp. Cell Res., 242, 487-494.
27. Balajee, A.S., Machwe, A., May, A., Gray, M.D., Oshima, J., Martin, G.M., Nehlin, J.O., Brosh, R.M., Jr, Orren, D.K. and Bohr, V.A. (1999) The Werner syndrome protein is involved in RNA polymerase II transcription. Mol. Biol. Cell, 10, 2655-2668.
28. Shiratori, M., Sakamoto, S., Suzuki, N., Tokutake, Y., Kawabe, Y., Enomoto, T., Sugimoto, M., Goto, M., Matsumoto, T. and Furuichi, Y. (1999) Detection by epitope-defined monoclonal antibodies of Werner DNA helicases in the nucleoplasm and their upregulation by cell transformation and immortalization. J. Cell Biol., 144, 1-9.
29. Runger, T.M., Bauer, C., Dekant, B., Moller, K., Sobotta, P., Czerny, C., Poot, M. and Martin, G.M. (1994) Hypermutable ligation of plasmid DNA ends in cells from patients with Werner syndrome. J. Invest. Dermatol., 102, 45-48.
30. Yamaguchi-Iwai, Y., Sonoda, E., Sasaki, M.S., Morrison, C., Haraguchi, T., Hiraoka, Y., Yamashita, Y.M., Yagi, T., Takala, M., Price, C. et al. (1999) Mre11 is essential for the maintenance of chromosomal DNA in vertebrate cells. EMBO J., 18, 6619-6629.
31. Friedberg, E.C., Walker, G.C. and Siede, W. (1995) DNA Repair and Mutagenesis. ASM Press, Washington, DC.